Ancestral gene acquisition as the key to virulence potential in environmental Vibrio populations
Diseases of marine animals caused by bacteria of the genus Vibrio are on the rise worldwide. Understanding the eco-evolutionary dynamics of these infectious agents is important for predicting and managing these diseases. Yet, compared to Vibrio infecting humans, knowledge of their role as animal pat...
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ftarchimer:oai:archimer.ifremer.fr:56370 2023-05-15T15:58:40+02:00 Ancestral gene acquisition as the key to virulence potential in environmental Vibrio populations Bruto, Maxime Labreuche, Yannick James, Adele Piel, Damien Chenivesse, Sabine Petton, Bruno Polz, Martin F. Le Roux, Frederique 2018-12 application/pdf https://archimer.ifremer.fr/doc/00452/56370/57967.pdf https://archimer.ifremer.fr/doc/00452/56370/57968.pdf https://doi.org/10.1038/s41396-018-0245-3 https://archimer.ifremer.fr/doc/00452/56370/ eng eng Nature Publishing Group https://archimer.ifremer.fr/doc/00452/56370/57967.pdf https://archimer.ifremer.fr/doc/00452/56370/57968.pdf doi:10.1038/s41396-018-0245-3 https://archimer.ifremer.fr/doc/00452/56370/ The Author(s) 2018. This article is published with open access info:eu-repo/semantics/openAccess restricted use Isme Journal (1751-7362) (Nature Publishing Group), 2018-12 , Vol. 12 , N. 12 , P. 2954-2966 text Publication info:eu-repo/semantics/article 2018 ftarchimer https://doi.org/10.1038/s41396-018-0245-3 2021-09-23T20:31:29Z Diseases of marine animals caused by bacteria of the genus Vibrio are on the rise worldwide. Understanding the eco-evolutionary dynamics of these infectious agents is important for predicting and managing these diseases. Yet, compared to Vibrio infecting humans, knowledge of their role as animal pathogens is scarce. Here we ask how widespread is virulence among ecologically differentiated Vibrio populations, and what is the nature and frequency of virulence genes within these populations? We use a combination of population genomics and molecular genetics to assay hundreds of Vibrio strains for their virulence in the oyster Crassostrea gigas, a unique animal model that allows high-throughput infection assays. We show that within the diverse Splendidus clade, virulence represents an ancestral trait but has been lost from several populations. Two loci are necessary for virulence, the first being widely distributed across the Splendidus clade and consisting of an exported conserved protein (R5.7). The second is a MARTX toxin cluster, which only occurs within V. splendidus and is for the first time associated with virulence in marine invertebrates. Varying frequencies of both loci among populations indicate different selective pressures and alternative ecological roles, based on which we suggest strategies for epidemiological surveys. Article in Journal/Newspaper Crassostrea gigas Archimer (Archive Institutionnelle de l'Ifremer - Institut français de recherche pour l'exploitation de la mer) The ISME Journal 12 12 2954 2966 |
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Archimer (Archive Institutionnelle de l'Ifremer - Institut français de recherche pour l'exploitation de la mer) |
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ftarchimer |
language |
English |
description |
Diseases of marine animals caused by bacteria of the genus Vibrio are on the rise worldwide. Understanding the eco-evolutionary dynamics of these infectious agents is important for predicting and managing these diseases. Yet, compared to Vibrio infecting humans, knowledge of their role as animal pathogens is scarce. Here we ask how widespread is virulence among ecologically differentiated Vibrio populations, and what is the nature and frequency of virulence genes within these populations? We use a combination of population genomics and molecular genetics to assay hundreds of Vibrio strains for their virulence in the oyster Crassostrea gigas, a unique animal model that allows high-throughput infection assays. We show that within the diverse Splendidus clade, virulence represents an ancestral trait but has been lost from several populations. Two loci are necessary for virulence, the first being widely distributed across the Splendidus clade and consisting of an exported conserved protein (R5.7). The second is a MARTX toxin cluster, which only occurs within V. splendidus and is for the first time associated with virulence in marine invertebrates. Varying frequencies of both loci among populations indicate different selective pressures and alternative ecological roles, based on which we suggest strategies for epidemiological surveys. |
format |
Article in Journal/Newspaper |
author |
Bruto, Maxime Labreuche, Yannick James, Adele Piel, Damien Chenivesse, Sabine Petton, Bruno Polz, Martin F. Le Roux, Frederique |
spellingShingle |
Bruto, Maxime Labreuche, Yannick James, Adele Piel, Damien Chenivesse, Sabine Petton, Bruno Polz, Martin F. Le Roux, Frederique Ancestral gene acquisition as the key to virulence potential in environmental Vibrio populations |
author_facet |
Bruto, Maxime Labreuche, Yannick James, Adele Piel, Damien Chenivesse, Sabine Petton, Bruno Polz, Martin F. Le Roux, Frederique |
author_sort |
Bruto, Maxime |
title |
Ancestral gene acquisition as the key to virulence potential in environmental Vibrio populations |
title_short |
Ancestral gene acquisition as the key to virulence potential in environmental Vibrio populations |
title_full |
Ancestral gene acquisition as the key to virulence potential in environmental Vibrio populations |
title_fullStr |
Ancestral gene acquisition as the key to virulence potential in environmental Vibrio populations |
title_full_unstemmed |
Ancestral gene acquisition as the key to virulence potential in environmental Vibrio populations |
title_sort |
ancestral gene acquisition as the key to virulence potential in environmental vibrio populations |
publisher |
Nature Publishing Group |
publishDate |
2018 |
url |
https://archimer.ifremer.fr/doc/00452/56370/57967.pdf https://archimer.ifremer.fr/doc/00452/56370/57968.pdf https://doi.org/10.1038/s41396-018-0245-3 https://archimer.ifremer.fr/doc/00452/56370/ |
genre |
Crassostrea gigas |
genre_facet |
Crassostrea gigas |
op_source |
Isme Journal (1751-7362) (Nature Publishing Group), 2018-12 , Vol. 12 , N. 12 , P. 2954-2966 |
op_relation |
https://archimer.ifremer.fr/doc/00452/56370/57967.pdf https://archimer.ifremer.fr/doc/00452/56370/57968.pdf doi:10.1038/s41396-018-0245-3 https://archimer.ifremer.fr/doc/00452/56370/ |
op_rights |
The Author(s) 2018. This article is published with open access info:eu-repo/semantics/openAccess restricted use |
op_doi |
https://doi.org/10.1038/s41396-018-0245-3 |
container_title |
The ISME Journal |
container_volume |
12 |
container_issue |
12 |
container_start_page |
2954 |
op_container_end_page |
2966 |
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1766394443791859712 |