pH controls spermatozoa motility in the Pacific oyster ( Crassostrea gigas )
Investigating the roles of chemical factors stimulating and inhibiting sperm motility is required to understand the mechanisms of spermatozoa movement. In this study, we described the composition of the seminal fluid (osmotic pressure, pH, and ions) and investigated the roles of these factors and sa...
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ftarchimer:oai:archimer.ifremer.fr:53976 2023-05-15T15:58:40+02:00 pH controls spermatozoa motility in the Pacific oyster ( Crassostrea gigas ) Boulais, Myrina Suquet, Marc Arsenault-pernet, Marie Eve Julie Malo, Florent Queau, Isabelle Pignet, Patricia Ratiskol, Dominique Le Grand, Jacqueline Huber, Matthias Cosson, Jacky 2018-03 application/pdf https://archimer.ifremer.fr/doc/00428/53976/55137.pdf https://doi.org/10.1242/bio.031427 https://archimer.ifremer.fr/doc/00428/53976/ eng eng Company Of Biologists Ltd https://archimer.ifremer.fr/doc/00428/53976/55137.pdf doi:10.1242/bio.031427 https://archimer.ifremer.fr/doc/00428/53976/ 2018. Published by The Company of Biologists Ltd info:eu-repo/semantics/openAccess restricted use Biology Open (2046-6390) (Company Of Biologists Ltd), 2018-03 , Vol. 7 , N. 3 , P. bio031427 (8p.) Seminal fluid Spermatozoa Motility pH Salinity Ions text Publication info:eu-repo/semantics/article 2018 ftarchimer https://doi.org/10.1242/bio.031427 2021-09-23T20:30:38Z Investigating the roles of chemical factors stimulating and inhibiting sperm motility is required to understand the mechanisms of spermatozoa movement. In this study, we described the composition of the seminal fluid (osmotic pressure, pH, and ions) and investigated the roles of these factors and salinity in initiating spermatozoa movement in the Pacific oyster. The acidic pH of the gonad (5.82 ± 0.22) maintained sperm in the quiescent stage and initiation of flagellar movement was triggered by a sudden increase of spermatozoa external pH (pHe) when released in seawater (SW). At pH 6.4, percentage of motile spermatozoa was three times higher when they were activated in SW containing 30 mM NH4Cl, which alkalinizes internal pH (pHi) of spermatozoa, compared to NH4Cl-free SW, revealing the role of pHi in triggering sperm movement. Percentage of motile spermatozoa activated in Na+-free artificial seawater (ASW) was highly reduced compared to ASW, suggesting that change of pHi triggering sperm motility was mediated by a Na+/H+ exchanger. Motility and swimming speed were highest in salinities between 33.8 and 42.7‰ (within a range of 0 to 50 ‰), and pH values above 7.5 (within a range of 4.5 to 9.5). Article in Journal/Newspaper Crassostrea gigas Pacific oyster Archimer (Archive Institutionnelle de l'Ifremer - Institut français de recherche pour l'exploitation de la mer) Pacific Biology Open |
institution |
Open Polar |
collection |
Archimer (Archive Institutionnelle de l'Ifremer - Institut français de recherche pour l'exploitation de la mer) |
op_collection_id |
ftarchimer |
language |
English |
topic |
Seminal fluid Spermatozoa Motility pH Salinity Ions |
spellingShingle |
Seminal fluid Spermatozoa Motility pH Salinity Ions Boulais, Myrina Suquet, Marc Arsenault-pernet, Marie Eve Julie Malo, Florent Queau, Isabelle Pignet, Patricia Ratiskol, Dominique Le Grand, Jacqueline Huber, Matthias Cosson, Jacky pH controls spermatozoa motility in the Pacific oyster ( Crassostrea gigas ) |
topic_facet |
Seminal fluid Spermatozoa Motility pH Salinity Ions |
description |
Investigating the roles of chemical factors stimulating and inhibiting sperm motility is required to understand the mechanisms of spermatozoa movement. In this study, we described the composition of the seminal fluid (osmotic pressure, pH, and ions) and investigated the roles of these factors and salinity in initiating spermatozoa movement in the Pacific oyster. The acidic pH of the gonad (5.82 ± 0.22) maintained sperm in the quiescent stage and initiation of flagellar movement was triggered by a sudden increase of spermatozoa external pH (pHe) when released in seawater (SW). At pH 6.4, percentage of motile spermatozoa was three times higher when they were activated in SW containing 30 mM NH4Cl, which alkalinizes internal pH (pHi) of spermatozoa, compared to NH4Cl-free SW, revealing the role of pHi in triggering sperm movement. Percentage of motile spermatozoa activated in Na+-free artificial seawater (ASW) was highly reduced compared to ASW, suggesting that change of pHi triggering sperm motility was mediated by a Na+/H+ exchanger. Motility and swimming speed were highest in salinities between 33.8 and 42.7‰ (within a range of 0 to 50 ‰), and pH values above 7.5 (within a range of 4.5 to 9.5). |
format |
Article in Journal/Newspaper |
author |
Boulais, Myrina Suquet, Marc Arsenault-pernet, Marie Eve Julie Malo, Florent Queau, Isabelle Pignet, Patricia Ratiskol, Dominique Le Grand, Jacqueline Huber, Matthias Cosson, Jacky |
author_facet |
Boulais, Myrina Suquet, Marc Arsenault-pernet, Marie Eve Julie Malo, Florent Queau, Isabelle Pignet, Patricia Ratiskol, Dominique Le Grand, Jacqueline Huber, Matthias Cosson, Jacky |
author_sort |
Boulais, Myrina |
title |
pH controls spermatozoa motility in the Pacific oyster ( Crassostrea gigas ) |
title_short |
pH controls spermatozoa motility in the Pacific oyster ( Crassostrea gigas ) |
title_full |
pH controls spermatozoa motility in the Pacific oyster ( Crassostrea gigas ) |
title_fullStr |
pH controls spermatozoa motility in the Pacific oyster ( Crassostrea gigas ) |
title_full_unstemmed |
pH controls spermatozoa motility in the Pacific oyster ( Crassostrea gigas ) |
title_sort |
ph controls spermatozoa motility in the pacific oyster ( crassostrea gigas ) |
publisher |
Company Of Biologists Ltd |
publishDate |
2018 |
url |
https://archimer.ifremer.fr/doc/00428/53976/55137.pdf https://doi.org/10.1242/bio.031427 https://archimer.ifremer.fr/doc/00428/53976/ |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
Biology Open (2046-6390) (Company Of Biologists Ltd), 2018-03 , Vol. 7 , N. 3 , P. bio031427 (8p.) |
op_relation |
https://archimer.ifremer.fr/doc/00428/53976/55137.pdf doi:10.1242/bio.031427 https://archimer.ifremer.fr/doc/00428/53976/ |
op_rights |
2018. Published by The Company of Biologists Ltd info:eu-repo/semantics/openAccess restricted use |
op_doi |
https://doi.org/10.1242/bio.031427 |
container_title |
Biology Open |
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1766394443615698944 |