The Voltage-Dependent Anion Channel (VDAC) of Pacific Oysters Crassostrea gigas Is Upaccumulated During Infection by the Ostreid Herpesvirus-1 (OsHV-1): an Indicator of the Warburg Effect
Voltage-dependent anion channel (VDAC) is a key mitochondrial protein. VDAC drives cellular energy metabolism by controlling the influx and efflux of metabolites and ions through the mitochondrial membrane, playing a role in its permeabilization. This protein exerts a pivotal role during the white s...
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ftarchimer:oai:archimer.ifremer.fr:53129 2023-05-15T15:57:42+02:00 The Voltage-Dependent Anion Channel (VDAC) of Pacific Oysters Crassostrea gigas Is Upaccumulated During Infection by the Ostreid Herpesvirus-1 (OsHV-1): an Indicator of the Warburg Effect Delisle, Lizenn Fuhrmann, Marine Quere, Claudie Pauletto, Marianna Pichereau, Vianney Pernet, Fabrice Corporeau, Charlotte 2018-02 application/pdf https://archimer.ifremer.fr/doc/00420/53129/54142.pdf https://doi.org/10.1007/s10126-017-9789-x https://archimer.ifremer.fr/doc/00420/53129/ eng eng Springer https://archimer.ifremer.fr/doc/00420/53129/54142.pdf doi:10.1007/s10126-017-9789-x https://archimer.ifremer.fr/doc/00420/53129/ Springer Science+Business Media, LLC, part of Springer Nature 2018 info:eu-repo/semantics/openAccess restricted use Marine Biotechnology (1436-2228) (Springer), 2018-02 , Vol. 20 , N. 1 , P. 87-97 Voltage-dependent anion channel Warburg effect Crassostrea gigas Ostreid herpes virus text Publication info:eu-repo/semantics/article 2018 ftarchimer https://doi.org/10.1007/s10126-017-9789-x 2021-09-23T20:30:24Z Voltage-dependent anion channel (VDAC) is a key mitochondrial protein. VDAC drives cellular energy metabolism by controlling the influx and efflux of metabolites and ions through the mitochondrial membrane, playing a role in its permeabilization. This protein exerts a pivotal role during the white spot syndrome virus (WSSV) infection in shrimp, through its involvement in a particular metabolism that plays in favor of the virus, the Warburg effect. The Warburg effect corresponds to an atypical metabolic shift toward an aerobic glycolysis that provides energy for rapid cell division and resistance to apoptosis. In the Pacific oyster Crassostrea gigas, the Warburg effect occurs during infection by Ostreid herpesvirus (OsHV-1). At present, the role of VDAC in the Warburg effect, OsHV-1 infection and apoptosis is unknown. Here, we developed a specific antibody directed against C. gigas VDAC. This tool allowed us to quantify the tissue-specific expression of VDAC, to detect VDAC oligomers, and to follow the amount of VDAC in oysters deployed in the field. We showed that oysters sensitive to a mortality event in the field presented an accumulation of VDAC. Finally, we propose to use VDAC quantification as a tool to measure the oyster susceptibility to OsHV-1 depending on its environment. Article in Journal/Newspaper Crassostrea gigas Pacific oyster Archimer (Archive Institutionnelle de l'Ifremer - Institut français de recherche pour l'exploitation de la mer) Pacific Marine Biotechnology 20 1 87 97 |
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Open Polar |
collection |
Archimer (Archive Institutionnelle de l'Ifremer - Institut français de recherche pour l'exploitation de la mer) |
op_collection_id |
ftarchimer |
language |
English |
topic |
Voltage-dependent anion channel Warburg effect Crassostrea gigas Ostreid herpes virus |
spellingShingle |
Voltage-dependent anion channel Warburg effect Crassostrea gigas Ostreid herpes virus Delisle, Lizenn Fuhrmann, Marine Quere, Claudie Pauletto, Marianna Pichereau, Vianney Pernet, Fabrice Corporeau, Charlotte The Voltage-Dependent Anion Channel (VDAC) of Pacific Oysters Crassostrea gigas Is Upaccumulated During Infection by the Ostreid Herpesvirus-1 (OsHV-1): an Indicator of the Warburg Effect |
topic_facet |
Voltage-dependent anion channel Warburg effect Crassostrea gigas Ostreid herpes virus |
description |
Voltage-dependent anion channel (VDAC) is a key mitochondrial protein. VDAC drives cellular energy metabolism by controlling the influx and efflux of metabolites and ions through the mitochondrial membrane, playing a role in its permeabilization. This protein exerts a pivotal role during the white spot syndrome virus (WSSV) infection in shrimp, through its involvement in a particular metabolism that plays in favor of the virus, the Warburg effect. The Warburg effect corresponds to an atypical metabolic shift toward an aerobic glycolysis that provides energy for rapid cell division and resistance to apoptosis. In the Pacific oyster Crassostrea gigas, the Warburg effect occurs during infection by Ostreid herpesvirus (OsHV-1). At present, the role of VDAC in the Warburg effect, OsHV-1 infection and apoptosis is unknown. Here, we developed a specific antibody directed against C. gigas VDAC. This tool allowed us to quantify the tissue-specific expression of VDAC, to detect VDAC oligomers, and to follow the amount of VDAC in oysters deployed in the field. We showed that oysters sensitive to a mortality event in the field presented an accumulation of VDAC. Finally, we propose to use VDAC quantification as a tool to measure the oyster susceptibility to OsHV-1 depending on its environment. |
format |
Article in Journal/Newspaper |
author |
Delisle, Lizenn Fuhrmann, Marine Quere, Claudie Pauletto, Marianna Pichereau, Vianney Pernet, Fabrice Corporeau, Charlotte |
author_facet |
Delisle, Lizenn Fuhrmann, Marine Quere, Claudie Pauletto, Marianna Pichereau, Vianney Pernet, Fabrice Corporeau, Charlotte |
author_sort |
Delisle, Lizenn |
title |
The Voltage-Dependent Anion Channel (VDAC) of Pacific Oysters Crassostrea gigas Is Upaccumulated During Infection by the Ostreid Herpesvirus-1 (OsHV-1): an Indicator of the Warburg Effect |
title_short |
The Voltage-Dependent Anion Channel (VDAC) of Pacific Oysters Crassostrea gigas Is Upaccumulated During Infection by the Ostreid Herpesvirus-1 (OsHV-1): an Indicator of the Warburg Effect |
title_full |
The Voltage-Dependent Anion Channel (VDAC) of Pacific Oysters Crassostrea gigas Is Upaccumulated During Infection by the Ostreid Herpesvirus-1 (OsHV-1): an Indicator of the Warburg Effect |
title_fullStr |
The Voltage-Dependent Anion Channel (VDAC) of Pacific Oysters Crassostrea gigas Is Upaccumulated During Infection by the Ostreid Herpesvirus-1 (OsHV-1): an Indicator of the Warburg Effect |
title_full_unstemmed |
The Voltage-Dependent Anion Channel (VDAC) of Pacific Oysters Crassostrea gigas Is Upaccumulated During Infection by the Ostreid Herpesvirus-1 (OsHV-1): an Indicator of the Warburg Effect |
title_sort |
voltage-dependent anion channel (vdac) of pacific oysters crassostrea gigas is upaccumulated during infection by the ostreid herpesvirus-1 (oshv-1): an indicator of the warburg effect |
publisher |
Springer |
publishDate |
2018 |
url |
https://archimer.ifremer.fr/doc/00420/53129/54142.pdf https://doi.org/10.1007/s10126-017-9789-x https://archimer.ifremer.fr/doc/00420/53129/ |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
Marine Biotechnology (1436-2228) (Springer), 2018-02 , Vol. 20 , N. 1 , P. 87-97 |
op_relation |
https://archimer.ifremer.fr/doc/00420/53129/54142.pdf doi:10.1007/s10126-017-9789-x https://archimer.ifremer.fr/doc/00420/53129/ |
op_rights |
Springer Science+Business Media, LLC, part of Springer Nature 2018 info:eu-repo/semantics/openAccess restricted use |
op_doi |
https://doi.org/10.1007/s10126-017-9789-x |
container_title |
Marine Biotechnology |
container_volume |
20 |
container_issue |
1 |
container_start_page |
87 |
op_container_end_page |
97 |
_version_ |
1766393381926207488 |