Early gametogenesis in the Pacific oyster: new insights using stem cell and mitotic markers
While our knowledge of bivalve gametogenesis has progressed in recent times, more molecular markers are needed in order to develop tissue imaging. Here, we identified stem cell and mitotic markers to further characterize oyster early gametogenesis, mainly through immunofluorescence microscopy. Inten...
Published in: | Journal of Experimental Biology |
---|---|
Main Authors: | , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Company Of Biologists Ltd
2017
|
Subjects: | |
Online Access: | https://archimer.ifremer.fr/doc/00409/52100/52809.pdf https://doi.org/10.1242/jeb.167734 https://archimer.ifremer.fr/doc/00409/52100/ |
id |
ftarchimer:oai:archimer.ifremer.fr:52100 |
---|---|
record_format |
openpolar |
spelling |
ftarchimer:oai:archimer.ifremer.fr:52100 2023-05-15T17:54:20+02:00 Early gametogenesis in the Pacific oyster: new insights using stem cell and mitotic markers Cavelier, Patricia Cau, Julien Morin, Nathalie Delsert, Claude 2017-11 application/pdf https://archimer.ifremer.fr/doc/00409/52100/52809.pdf https://doi.org/10.1242/jeb.167734 https://archimer.ifremer.fr/doc/00409/52100/ eng eng Company Of Biologists Ltd https://archimer.ifremer.fr/doc/00409/52100/52809.pdf doi:10.1242/jeb.167734 https://archimer.ifremer.fr/doc/00409/52100/ 2017. Published by The Company of Biologists Ltd info:eu-repo/semantics/openAccess restricted use Journal Of Experimental Biology (0022-0949) (Company Of Biologists Ltd), 2017-11 , Vol. 220 , N. 21 , P. 3988-3996 Germline cells Cell cycle Reproduction Bivalve Marine invertebrates text Publication info:eu-repo/semantics/article 2017 ftarchimer https://doi.org/10.1242/jeb.167734 2021-09-23T20:30:05Z While our knowledge of bivalve gametogenesis has progressed in recent times, more molecular markers are needed in order to develop tissue imaging. Here, we identified stem cell and mitotic markers to further characterize oyster early gametogenesis, mainly through immunofluorescence microscopy. Intense alkaline phosphatase activity, a non-specific marker for stem cells, was detected on the outer edge of the gonad ducts at the post-spawning stage, suggesting an abundance of undifferentiated cells very early during the sexual cycle. This observation was confirmed using an antibody against Sox2, a transcription factor specific for stem or germline cells, which labeled cells in the gonad duct inner mass and ciliated epithelium early during the initial oyster sexual cycle. Moreover, Vasa, a cytoplasmic marker for germline cells, was also detected in the gonad acini and duct cells, thus confirming that germline cells were abundant early on. In addition, the binding of the minichromosome maintenance MCM6 protein to chromatin indicated the gonad acini and duct cells were engaged in the cell cycle. DNA replication was indeed confirmed by an abundant in vivo incorporation of BrdU into the duct cell chromatin. Finally, proliferation of acini and duct cells was demonstrated by the chromatin-bound Ser10-phosphorylated histone H3, a mitotic marker. The markers for the cell cycle and mitosis used here thus indicate that acini and duct cells were already actively dividing early during the oyster sexual cycle. In addition, together with the stem cell markers, these data reveal that the epithelium delimiting the duct outer edge contains a dynamic population of undifferentiated cells. Article in Journal/Newspaper Pacific oyster Archimer (Archive Institutionnelle de l'Ifremer - Institut français de recherche pour l'exploitation de la mer) Pacific Vasa ENVELOPE(25.177,25.177,67.587,67.587) Journal of Experimental Biology |
institution |
Open Polar |
collection |
Archimer (Archive Institutionnelle de l'Ifremer - Institut français de recherche pour l'exploitation de la mer) |
op_collection_id |
ftarchimer |
language |
English |
topic |
Germline cells Cell cycle Reproduction Bivalve Marine invertebrates |
spellingShingle |
Germline cells Cell cycle Reproduction Bivalve Marine invertebrates Cavelier, Patricia Cau, Julien Morin, Nathalie Delsert, Claude Early gametogenesis in the Pacific oyster: new insights using stem cell and mitotic markers |
topic_facet |
Germline cells Cell cycle Reproduction Bivalve Marine invertebrates |
description |
While our knowledge of bivalve gametogenesis has progressed in recent times, more molecular markers are needed in order to develop tissue imaging. Here, we identified stem cell and mitotic markers to further characterize oyster early gametogenesis, mainly through immunofluorescence microscopy. Intense alkaline phosphatase activity, a non-specific marker for stem cells, was detected on the outer edge of the gonad ducts at the post-spawning stage, suggesting an abundance of undifferentiated cells very early during the sexual cycle. This observation was confirmed using an antibody against Sox2, a transcription factor specific for stem or germline cells, which labeled cells in the gonad duct inner mass and ciliated epithelium early during the initial oyster sexual cycle. Moreover, Vasa, a cytoplasmic marker for germline cells, was also detected in the gonad acini and duct cells, thus confirming that germline cells were abundant early on. In addition, the binding of the minichromosome maintenance MCM6 protein to chromatin indicated the gonad acini and duct cells were engaged in the cell cycle. DNA replication was indeed confirmed by an abundant in vivo incorporation of BrdU into the duct cell chromatin. Finally, proliferation of acini and duct cells was demonstrated by the chromatin-bound Ser10-phosphorylated histone H3, a mitotic marker. The markers for the cell cycle and mitosis used here thus indicate that acini and duct cells were already actively dividing early during the oyster sexual cycle. In addition, together with the stem cell markers, these data reveal that the epithelium delimiting the duct outer edge contains a dynamic population of undifferentiated cells. |
format |
Article in Journal/Newspaper |
author |
Cavelier, Patricia Cau, Julien Morin, Nathalie Delsert, Claude |
author_facet |
Cavelier, Patricia Cau, Julien Morin, Nathalie Delsert, Claude |
author_sort |
Cavelier, Patricia |
title |
Early gametogenesis in the Pacific oyster: new insights using stem cell and mitotic markers |
title_short |
Early gametogenesis in the Pacific oyster: new insights using stem cell and mitotic markers |
title_full |
Early gametogenesis in the Pacific oyster: new insights using stem cell and mitotic markers |
title_fullStr |
Early gametogenesis in the Pacific oyster: new insights using stem cell and mitotic markers |
title_full_unstemmed |
Early gametogenesis in the Pacific oyster: new insights using stem cell and mitotic markers |
title_sort |
early gametogenesis in the pacific oyster: new insights using stem cell and mitotic markers |
publisher |
Company Of Biologists Ltd |
publishDate |
2017 |
url |
https://archimer.ifremer.fr/doc/00409/52100/52809.pdf https://doi.org/10.1242/jeb.167734 https://archimer.ifremer.fr/doc/00409/52100/ |
long_lat |
ENVELOPE(25.177,25.177,67.587,67.587) |
geographic |
Pacific Vasa |
geographic_facet |
Pacific Vasa |
genre |
Pacific oyster |
genre_facet |
Pacific oyster |
op_source |
Journal Of Experimental Biology (0022-0949) (Company Of Biologists Ltd), 2017-11 , Vol. 220 , N. 21 , P. 3988-3996 |
op_relation |
https://archimer.ifremer.fr/doc/00409/52100/52809.pdf doi:10.1242/jeb.167734 https://archimer.ifremer.fr/doc/00409/52100/ |
op_rights |
2017. Published by The Company of Biologists Ltd info:eu-repo/semantics/openAccess restricted use |
op_doi |
https://doi.org/10.1242/jeb.167734 |
container_title |
Journal of Experimental Biology |
_version_ |
1766162096156835840 |