The paralytic shellfish toxin, saxitoxin, enters the cytoplasm and induces apoptosis of oyster immune cells through a caspase-dependent pathway
Exposure of the toxin-producing dinoflagellate Alexandrium catenella (A. catenella) was previously demonstrated to cause apoptosis of hemocytes in the oyster species Crassostrea gigas. In this work, a coumarin-labeled saxitoxin appeared to spread throughout the cytoplasm of the hemocytes. PSTs, incl...
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ftarchimer:oai:archimer.ifremer.fr:50272 2023-05-15T15:58:40+02:00 The paralytic shellfish toxin, saxitoxin, enters the cytoplasm and induces apoptosis of oyster immune cells through a caspase-dependent pathway Abikhalil, Celina Finkelstein, Darren S. Conejero, Genevieve Du Bois, Justin Destoumieux-garzon, Delphine Rolland, Jean-luc 2017-09 application/pdf https://archimer.ifremer.fr/doc/00391/50272/50905.pdf https://doi.org/10.1016/j.aquatox.2017.07.001 https://archimer.ifremer.fr/doc/00391/50272/ eng eng Elsevier Science Bv https://archimer.ifremer.fr/doc/00391/50272/50905.pdf doi:10.1016/j.aquatox.2017.07.001 https://archimer.ifremer.fr/doc/00391/50272/ 2017 Elsevier B.V. All rights reserved. info:eu-repo/semantics/openAccess restricted use Aquatic Toxicology (0166-445X) (Elsevier Science Bv), 2017-09 , Vol. 190 , P. 133-141 Shellfish Toxin Harmful algae Phytoplankton text Publication info:eu-repo/semantics/article 2017 ftarchimer https://doi.org/10.1016/j.aquatox.2017.07.001 2021-09-23T20:29:44Z Exposure of the toxin-producing dinoflagellate Alexandrium catenella (A. catenella) was previously demonstrated to cause apoptosis of hemocytes in the oyster species Crassostrea gigas. In this work, a coumarin-labeled saxitoxin appeared to spread throughout the cytoplasm of the hemocytes. PSTs, including saxitoxin, were also shown to be directly responsible for inducing apoptosis in hemocytes, a process dependent on caspase activation and independent of reactive oxygen species (ROS) production. A series of in vitro labeling and microscopy experiments revealed that STX and analogs there of induced nuclear condensation, phosphatidylserine exposure, membrane permeability, and DNA fragmentation of hemocytes. Unlike in vertebrates, gonyautoxin-5 (GTX5), which is present in high concentrations in A. catenella, was found to be more toxic than saxitoxin (STX) to oyster immune cells. Altogether, results show that PSTs produced by toxic dinoflagellates enter the cytoplasm and induce apoptosis of oyster immune cells through a caspase-dependent pathway. Because of the central role of hemocytes in mollusc immune defense, PST-induced death of hemocytes could negatively affect resistance of bivalve molluscs to microbial infection. Article in Journal/Newspaper Crassostrea gigas Archimer (Archive Institutionnelle de l'Ifremer - Institut français de recherche pour l'exploitation de la mer) Aquatic Toxicology 190 133 141 |
institution |
Open Polar |
collection |
Archimer (Archive Institutionnelle de l'Ifremer - Institut français de recherche pour l'exploitation de la mer) |
op_collection_id |
ftarchimer |
language |
English |
topic |
Shellfish Toxin Harmful algae Phytoplankton |
spellingShingle |
Shellfish Toxin Harmful algae Phytoplankton Abikhalil, Celina Finkelstein, Darren S. Conejero, Genevieve Du Bois, Justin Destoumieux-garzon, Delphine Rolland, Jean-luc The paralytic shellfish toxin, saxitoxin, enters the cytoplasm and induces apoptosis of oyster immune cells through a caspase-dependent pathway |
topic_facet |
Shellfish Toxin Harmful algae Phytoplankton |
description |
Exposure of the toxin-producing dinoflagellate Alexandrium catenella (A. catenella) was previously demonstrated to cause apoptosis of hemocytes in the oyster species Crassostrea gigas. In this work, a coumarin-labeled saxitoxin appeared to spread throughout the cytoplasm of the hemocytes. PSTs, including saxitoxin, were also shown to be directly responsible for inducing apoptosis in hemocytes, a process dependent on caspase activation and independent of reactive oxygen species (ROS) production. A series of in vitro labeling and microscopy experiments revealed that STX and analogs there of induced nuclear condensation, phosphatidylserine exposure, membrane permeability, and DNA fragmentation of hemocytes. Unlike in vertebrates, gonyautoxin-5 (GTX5), which is present in high concentrations in A. catenella, was found to be more toxic than saxitoxin (STX) to oyster immune cells. Altogether, results show that PSTs produced by toxic dinoflagellates enter the cytoplasm and induce apoptosis of oyster immune cells through a caspase-dependent pathway. Because of the central role of hemocytes in mollusc immune defense, PST-induced death of hemocytes could negatively affect resistance of bivalve molluscs to microbial infection. |
format |
Article in Journal/Newspaper |
author |
Abikhalil, Celina Finkelstein, Darren S. Conejero, Genevieve Du Bois, Justin Destoumieux-garzon, Delphine Rolland, Jean-luc |
author_facet |
Abikhalil, Celina Finkelstein, Darren S. Conejero, Genevieve Du Bois, Justin Destoumieux-garzon, Delphine Rolland, Jean-luc |
author_sort |
Abikhalil, Celina |
title |
The paralytic shellfish toxin, saxitoxin, enters the cytoplasm and induces apoptosis of oyster immune cells through a caspase-dependent pathway |
title_short |
The paralytic shellfish toxin, saxitoxin, enters the cytoplasm and induces apoptosis of oyster immune cells through a caspase-dependent pathway |
title_full |
The paralytic shellfish toxin, saxitoxin, enters the cytoplasm and induces apoptosis of oyster immune cells through a caspase-dependent pathway |
title_fullStr |
The paralytic shellfish toxin, saxitoxin, enters the cytoplasm and induces apoptosis of oyster immune cells through a caspase-dependent pathway |
title_full_unstemmed |
The paralytic shellfish toxin, saxitoxin, enters the cytoplasm and induces apoptosis of oyster immune cells through a caspase-dependent pathway |
title_sort |
paralytic shellfish toxin, saxitoxin, enters the cytoplasm and induces apoptosis of oyster immune cells through a caspase-dependent pathway |
publisher |
Elsevier Science Bv |
publishDate |
2017 |
url |
https://archimer.ifremer.fr/doc/00391/50272/50905.pdf https://doi.org/10.1016/j.aquatox.2017.07.001 https://archimer.ifremer.fr/doc/00391/50272/ |
genre |
Crassostrea gigas |
genre_facet |
Crassostrea gigas |
op_source |
Aquatic Toxicology (0166-445X) (Elsevier Science Bv), 2017-09 , Vol. 190 , P. 133-141 |
op_relation |
https://archimer.ifremer.fr/doc/00391/50272/50905.pdf doi:10.1016/j.aquatox.2017.07.001 https://archimer.ifremer.fr/doc/00391/50272/ |
op_rights |
2017 Elsevier B.V. All rights reserved. info:eu-repo/semantics/openAccess restricted use |
op_doi |
https://doi.org/10.1016/j.aquatox.2017.07.001 |
container_title |
Aquatic Toxicology |
container_volume |
190 |
container_start_page |
133 |
op_container_end_page |
141 |
_version_ |
1766394443083022336 |