ATP content and viability of spermatozoa drive variability of fertilization success in the Pacific oyster ( Crassostrea gigas )
Fertilization of the oocyte is a critical process of sexual reproduction depending among other factors on spermatozoa functionality. Mitochondria participate in many crucial processes for spermatozoa motility and fertilizing ability. The Pacific oyster, Crassostrea gigas, is characterized by a high...
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ftarchimer:oai:archimer.ifremer.fr:49769 2023-05-15T15:58:01+02:00 ATP content and viability of spermatozoa drive variability of fertilization success in the Pacific oyster ( Crassostrea gigas ) Boulais, Myrina Soudant, Philippe Le Goic, Nelly Quere, Claudie Boudry, Pierre Suquet, Marc 2017-10 application/pdf https://archimer.ifremer.fr/doc/00386/49769/50309.pdf https://doi.org/10.1016/j.aquaculture.2017.05.035 https://archimer.ifremer.fr/doc/00386/49769/ eng eng Elsevier Science Bv info:eu-repo/grantAgreement/EC/FP7/245119/EU//REPROSEED https://archimer.ifremer.fr/doc/00386/49769/50309.pdf doi:10.1016/j.aquaculture.2017.05.035 https://archimer.ifremer.fr/doc/00386/49769/ 2017 Elsevier B.V. All rights reserved. info:eu-repo/semantics/openAccess restricted use Aquaculture (0044-8486) (Elsevier Science Bv), 2017-10 , Vol. 479 , P. 114-119 Spermatozoa Quality Motility Viability ATP content ROS production text Publication info:eu-repo/semantics/article 2017 ftarchimer https://doi.org/10.1016/j.aquaculture.2017.05.035 2021-09-23T20:29:35Z Fertilization of the oocyte is a critical process of sexual reproduction depending among other factors on spermatozoa functionality. Mitochondria participate in many crucial processes for spermatozoa motility and fertilizing ability. The Pacific oyster, Crassostrea gigas, is characterized by a high inter-individual variability of its reproductive success which has been shown to be partially due to the variability of gamete quality. The present study explored spermatozoa characteristics such as: i) relationships among mitochondrial functionality (ATP content, mitochondrial membrane potential (MMP), reactive oxygen species production); sperm viability (percentages of live, dying, and dead); and motility; and ii) their involvement in inter-oyster variability of fertilizing ability demonstrated using simple and multiple regressions. Our results showed that ATP content depends on both MMP and viability of spermatozoa. Using multiple regressions, 61% of the variability of the trochophore-larval yield was explained by a model combining the ATP content and the percentage of dying spermatozoa (P < 0.001). Our results reveal that capacity of spermatozoa to maintain a high level of ATP via OXPHOS partly explains the inter-individual variability of fertilization success in the Pacific oyster. Sperm ATP content and viability assays will provide valuable tools for assessing sperm quality of this species in aquaculture production, cryopreservation, and bioassays. Article in Journal/Newspaper Crassostrea gigas Pacific oyster Archimer (Archive Institutionnelle de l'Ifremer - Institut français de recherche pour l'exploitation de la mer) Pacific Aquaculture 479 114 119 |
institution |
Open Polar |
collection |
Archimer (Archive Institutionnelle de l'Ifremer - Institut français de recherche pour l'exploitation de la mer) |
op_collection_id |
ftarchimer |
language |
English |
topic |
Spermatozoa Quality Motility Viability ATP content ROS production |
spellingShingle |
Spermatozoa Quality Motility Viability ATP content ROS production Boulais, Myrina Soudant, Philippe Le Goic, Nelly Quere, Claudie Boudry, Pierre Suquet, Marc ATP content and viability of spermatozoa drive variability of fertilization success in the Pacific oyster ( Crassostrea gigas ) |
topic_facet |
Spermatozoa Quality Motility Viability ATP content ROS production |
description |
Fertilization of the oocyte is a critical process of sexual reproduction depending among other factors on spermatozoa functionality. Mitochondria participate in many crucial processes for spermatozoa motility and fertilizing ability. The Pacific oyster, Crassostrea gigas, is characterized by a high inter-individual variability of its reproductive success which has been shown to be partially due to the variability of gamete quality. The present study explored spermatozoa characteristics such as: i) relationships among mitochondrial functionality (ATP content, mitochondrial membrane potential (MMP), reactive oxygen species production); sperm viability (percentages of live, dying, and dead); and motility; and ii) their involvement in inter-oyster variability of fertilizing ability demonstrated using simple and multiple regressions. Our results showed that ATP content depends on both MMP and viability of spermatozoa. Using multiple regressions, 61% of the variability of the trochophore-larval yield was explained by a model combining the ATP content and the percentage of dying spermatozoa (P < 0.001). Our results reveal that capacity of spermatozoa to maintain a high level of ATP via OXPHOS partly explains the inter-individual variability of fertilization success in the Pacific oyster. Sperm ATP content and viability assays will provide valuable tools for assessing sperm quality of this species in aquaculture production, cryopreservation, and bioassays. |
format |
Article in Journal/Newspaper |
author |
Boulais, Myrina Soudant, Philippe Le Goic, Nelly Quere, Claudie Boudry, Pierre Suquet, Marc |
author_facet |
Boulais, Myrina Soudant, Philippe Le Goic, Nelly Quere, Claudie Boudry, Pierre Suquet, Marc |
author_sort |
Boulais, Myrina |
title |
ATP content and viability of spermatozoa drive variability of fertilization success in the Pacific oyster ( Crassostrea gigas ) |
title_short |
ATP content and viability of spermatozoa drive variability of fertilization success in the Pacific oyster ( Crassostrea gigas ) |
title_full |
ATP content and viability of spermatozoa drive variability of fertilization success in the Pacific oyster ( Crassostrea gigas ) |
title_fullStr |
ATP content and viability of spermatozoa drive variability of fertilization success in the Pacific oyster ( Crassostrea gigas ) |
title_full_unstemmed |
ATP content and viability of spermatozoa drive variability of fertilization success in the Pacific oyster ( Crassostrea gigas ) |
title_sort |
atp content and viability of spermatozoa drive variability of fertilization success in the pacific oyster ( crassostrea gigas ) |
publisher |
Elsevier Science Bv |
publishDate |
2017 |
url |
https://archimer.ifremer.fr/doc/00386/49769/50309.pdf https://doi.org/10.1016/j.aquaculture.2017.05.035 https://archimer.ifremer.fr/doc/00386/49769/ |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
Aquaculture (0044-8486) (Elsevier Science Bv), 2017-10 , Vol. 479 , P. 114-119 |
op_relation |
info:eu-repo/grantAgreement/EC/FP7/245119/EU//REPROSEED https://archimer.ifremer.fr/doc/00386/49769/50309.pdf doi:10.1016/j.aquaculture.2017.05.035 https://archimer.ifremer.fr/doc/00386/49769/ |
op_rights |
2017 Elsevier B.V. All rights reserved. info:eu-repo/semantics/openAccess restricted use |
op_doi |
https://doi.org/10.1016/j.aquaculture.2017.05.035 |
container_title |
Aquaculture |
container_volume |
479 |
container_start_page |
114 |
op_container_end_page |
119 |
_version_ |
1766393742465433600 |