Molecular and phenotypic characterization of Vibrio aestuarianus subsp francensis subsp nov., a pathogen of the oyster Crassostrea gigas
Eleven Vibrio isolates invading the hemolymph of live and moribund oysters (Crassostrea gigas) collected in the field and from a hatchery in France, were characterized by a polyphasic approach. Phylogenetic analysis of 16S rRNA, gyrB and toxR genes indicated high homogeneity between these strains an...
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ftarchimer:oai:archimer.ifremer.fr:4961 2023-05-15T15:57:44+02:00 Molecular and phenotypic characterization of Vibrio aestuarianus subsp francensis subsp nov., a pathogen of the oyster Crassostrea gigas Garnier, Matthieu Labreuche, Yannick Nicolas, Jean-louis 2008-10 application/pdf https://archimer.ifremer.fr/doc/2008/publication-4961.pdf https://doi.org/10.1016/j.syapm.2008.06.003 https://archimer.ifremer.fr/doc/00000/4961/ eng eng Elsevier https://archimer.ifremer.fr/doc/2008/publication-4961.pdf doi:10.1016/j.syapm.2008.06.003 https://archimer.ifremer.fr/doc/00000/4961/ 2008 Elsevier GmbH All rights reserved. info:eu-repo/semantics/openAccess restricted use Systematic and Applied Microbiology (0723-2020) (Elsevier), 2008-10 , Vol. 31 , N. 5 , P. 358-365 Polyphasic approach Summer mortalities Crassostrea gigas Pathogen French isolates Vibrio aestuarianus text Publication info:eu-repo/semantics/article 2008 ftarchimer https://doi.org/10.1016/j.syapm.2008.06.003 2021-09-23T20:16:22Z Eleven Vibrio isolates invading the hemolymph of live and moribund oysters (Crassostrea gigas) collected in the field and from a hatchery in France, were characterized by a polyphasic approach. Phylogenetic analysis of 16S rRNA, gyrB and toxR genes indicated high homogeneity between these strains and the Vibrio aestuarianus type strain (ATCC35048(T)), and confirmed previous 16S rRNA analysis. In contrast, DNA:DNA hybridization was from 61% to 100%, while phenotypic characters and virulence tests showed a large diversity between the strains. Nevertheless, several common characters allowed the isolates to be distinguished from the reference strain. On the basis of several distinct phenotypic characteristics, it is proposed to establish two subspecies within the V. aestuarianus spp. group, V. aestuarianus subsp. aestuarianus [D. Tison, R. Seidler, Vibrio aestuarianus: a new species from estuarine waters and shellfish, Int. J. Syst. Bacteriol. (1983) 699-702] and V. aestuarianus subsp. francensis for these French isolates. The characters that differentiate the new strains from V. aestuarianus subsp. aestuarianus(T) are virulence (positive for 63% of the isolates) and 12:0 fatty acid content. The colonies were smaller and uncoloured, whereas no growth occurred at 35 degrees C or on TCBS, and the strains did not utilize several substrates, including L-serine, alpha-cyclodextrin, D-mannitol, alpha-glycyl-L-aspartic acid, L-threonine and glucose-1-phosphate. (c) 2008 Elsevier GmbH. All rights reserved. Article in Journal/Newspaper Crassostrea gigas Archimer (Archive Institutionnelle de l'Ifremer - Institut français de recherche pour l'exploitation de la mer) Systematic and Applied Microbiology 31 5 358 365 |
institution |
Open Polar |
collection |
Archimer (Archive Institutionnelle de l'Ifremer - Institut français de recherche pour l'exploitation de la mer) |
op_collection_id |
ftarchimer |
language |
English |
topic |
Polyphasic approach Summer mortalities Crassostrea gigas Pathogen French isolates Vibrio aestuarianus |
spellingShingle |
Polyphasic approach Summer mortalities Crassostrea gigas Pathogen French isolates Vibrio aestuarianus Garnier, Matthieu Labreuche, Yannick Nicolas, Jean-louis Molecular and phenotypic characterization of Vibrio aestuarianus subsp francensis subsp nov., a pathogen of the oyster Crassostrea gigas |
topic_facet |
Polyphasic approach Summer mortalities Crassostrea gigas Pathogen French isolates Vibrio aestuarianus |
description |
Eleven Vibrio isolates invading the hemolymph of live and moribund oysters (Crassostrea gigas) collected in the field and from a hatchery in France, were characterized by a polyphasic approach. Phylogenetic analysis of 16S rRNA, gyrB and toxR genes indicated high homogeneity between these strains and the Vibrio aestuarianus type strain (ATCC35048(T)), and confirmed previous 16S rRNA analysis. In contrast, DNA:DNA hybridization was from 61% to 100%, while phenotypic characters and virulence tests showed a large diversity between the strains. Nevertheless, several common characters allowed the isolates to be distinguished from the reference strain. On the basis of several distinct phenotypic characteristics, it is proposed to establish two subspecies within the V. aestuarianus spp. group, V. aestuarianus subsp. aestuarianus [D. Tison, R. Seidler, Vibrio aestuarianus: a new species from estuarine waters and shellfish, Int. J. Syst. Bacteriol. (1983) 699-702] and V. aestuarianus subsp. francensis for these French isolates. The characters that differentiate the new strains from V. aestuarianus subsp. aestuarianus(T) are virulence (positive for 63% of the isolates) and 12:0 fatty acid content. The colonies were smaller and uncoloured, whereas no growth occurred at 35 degrees C or on TCBS, and the strains did not utilize several substrates, including L-serine, alpha-cyclodextrin, D-mannitol, alpha-glycyl-L-aspartic acid, L-threonine and glucose-1-phosphate. (c) 2008 Elsevier GmbH. All rights reserved. |
format |
Article in Journal/Newspaper |
author |
Garnier, Matthieu Labreuche, Yannick Nicolas, Jean-louis |
author_facet |
Garnier, Matthieu Labreuche, Yannick Nicolas, Jean-louis |
author_sort |
Garnier, Matthieu |
title |
Molecular and phenotypic characterization of Vibrio aestuarianus subsp francensis subsp nov., a pathogen of the oyster Crassostrea gigas |
title_short |
Molecular and phenotypic characterization of Vibrio aestuarianus subsp francensis subsp nov., a pathogen of the oyster Crassostrea gigas |
title_full |
Molecular and phenotypic characterization of Vibrio aestuarianus subsp francensis subsp nov., a pathogen of the oyster Crassostrea gigas |
title_fullStr |
Molecular and phenotypic characterization of Vibrio aestuarianus subsp francensis subsp nov., a pathogen of the oyster Crassostrea gigas |
title_full_unstemmed |
Molecular and phenotypic characterization of Vibrio aestuarianus subsp francensis subsp nov., a pathogen of the oyster Crassostrea gigas |
title_sort |
molecular and phenotypic characterization of vibrio aestuarianus subsp francensis subsp nov., a pathogen of the oyster crassostrea gigas |
publisher |
Elsevier |
publishDate |
2008 |
url |
https://archimer.ifremer.fr/doc/2008/publication-4961.pdf https://doi.org/10.1016/j.syapm.2008.06.003 https://archimer.ifremer.fr/doc/00000/4961/ |
genre |
Crassostrea gigas |
genre_facet |
Crassostrea gigas |
op_source |
Systematic and Applied Microbiology (0723-2020) (Elsevier), 2008-10 , Vol. 31 , N. 5 , P. 358-365 |
op_relation |
https://archimer.ifremer.fr/doc/2008/publication-4961.pdf doi:10.1016/j.syapm.2008.06.003 https://archimer.ifremer.fr/doc/00000/4961/ |
op_rights |
2008 Elsevier GmbH All rights reserved. info:eu-repo/semantics/openAccess restricted use |
op_doi |
https://doi.org/10.1016/j.syapm.2008.06.003 |
container_title |
Systematic and Applied Microbiology |
container_volume |
31 |
container_issue |
5 |
container_start_page |
358 |
op_container_end_page |
365 |
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1766393424494198784 |