Morphological and genetic diversity of Beaufort Sea diatoms with high contributions from the Chaetoceros neogracilis species complex
Seventy-five diatoms strains isolated from the Beaufort Sea (Canadian Arctic) in the summer of 2009 were characterized by light and electron microscopy (SEM and TEM) as well as 18S and 28S rRNA gene sequencing. These strains group into 20 genotypes and 17 morphotypes and are affiliated with the gene...
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ftarchimer:oai:archimer.ifremer.fr:46718 2023-05-15T13:50:50+02:00 Morphological and genetic diversity of Beaufort Sea diatoms with high contributions from the Chaetoceros neogracilis species complex Balzano, Sergio Percopo, Isabella Siano, Raffaele Gourvil, Priscillia Chanoine, Melanie Marie, Dominique Vaulot, Daniel Sarno, Diana 2017-02 application/pdf https://archimer.ifremer.fr/doc/00356/46718/46584.pdf https://doi.org/10.1111/jpy.12489 https://archimer.ifremer.fr/doc/00356/46718/ eng eng Wiley info:eu-repo/grantAgreement/EC/FP7/227799/EU//ASSEMBLE info:eu-repo/grantAgreement/EC/FP7/311975/EU//MACUMBA https://archimer.ifremer.fr/doc/00356/46718/46584.pdf doi:10.1111/jpy.12489 https://archimer.ifremer.fr/doc/00356/46718/ 2016 Phycological Society of America info:eu-repo/semantics/openAccess restricted use Journal Of Phycology (0022-3646) (Wiley), 2017-02 , Vol. 53 , N. 1 , P. 161-187 biogeography ITS ITS2 secondary structure LSU morphology phylogeny polar diatoms SSU text Publication info:eu-repo/semantics/article 2017 ftarchimer https://doi.org/10.1111/jpy.12489 2021-09-23T20:28:58Z Seventy-five diatoms strains isolated from the Beaufort Sea (Canadian Arctic) in the summer of 2009 were characterized by light and electron microscopy (SEM and TEM) as well as 18S and 28S rRNA gene sequencing. These strains group into 20 genotypes and 17 morphotypes and are affiliated with the genera Arcocellulus, Attheya, Chaetoceros, Cylindrotheca, Eucampia, Nitzschia, Porosira, Pseudo-nitzschia, Shionodiscus, Thalassiosira, Synedropsis. Most of the species have a distribution confined to the northern/polar area. Chaetoceros neogracilis and Chaetoceros gelidus were the most represented taxa. Strains of C. neogracilis were morphologically similar and shared identical 18S rRNA gene sequences, but belonged to four distinct genetic clades based on 28S rRNA, ITS-1 and ITS-2 phylogenies. Secondary structure prediction revealed that these four clades differ in hemi-compensatory base changes (HCBCs) in paired positions of the ITS-2, suggesting their inability to interbreed. Reproductively isolated C. neogracilis genotypes can thus co-occur in summer phytoplankton communities in the Beaufort Sea. Chaetoceros neogracilis generally occurred as single cells but can also form short colonies. It is phylogenetically distinct from an Antarctic species, erroneously identified in some previous studies as C. neogracilis but named here as Chaetoceros sp. This work provides taxonomically validated sequences for 20 Arctic diatom taxa, which will facilitate future metabarcoding studies on phytoplankton in this region. Article in Journal/Newspaper Antarc* Antarctic Arctic Beaufort Sea Phytoplankton Archimer (Archive Institutionnelle de l'Ifremer - Institut français de recherche pour l'exploitation de la mer) Antarctic Arctic Journal of Phycology 53 1 161 187 |
institution |
Open Polar |
collection |
Archimer (Archive Institutionnelle de l'Ifremer - Institut français de recherche pour l'exploitation de la mer) |
op_collection_id |
ftarchimer |
language |
English |
topic |
biogeography ITS ITS2 secondary structure LSU morphology phylogeny polar diatoms SSU |
spellingShingle |
biogeography ITS ITS2 secondary structure LSU morphology phylogeny polar diatoms SSU Balzano, Sergio Percopo, Isabella Siano, Raffaele Gourvil, Priscillia Chanoine, Melanie Marie, Dominique Vaulot, Daniel Sarno, Diana Morphological and genetic diversity of Beaufort Sea diatoms with high contributions from the Chaetoceros neogracilis species complex |
topic_facet |
biogeography ITS ITS2 secondary structure LSU morphology phylogeny polar diatoms SSU |
description |
Seventy-five diatoms strains isolated from the Beaufort Sea (Canadian Arctic) in the summer of 2009 were characterized by light and electron microscopy (SEM and TEM) as well as 18S and 28S rRNA gene sequencing. These strains group into 20 genotypes and 17 morphotypes and are affiliated with the genera Arcocellulus, Attheya, Chaetoceros, Cylindrotheca, Eucampia, Nitzschia, Porosira, Pseudo-nitzschia, Shionodiscus, Thalassiosira, Synedropsis. Most of the species have a distribution confined to the northern/polar area. Chaetoceros neogracilis and Chaetoceros gelidus were the most represented taxa. Strains of C. neogracilis were morphologically similar and shared identical 18S rRNA gene sequences, but belonged to four distinct genetic clades based on 28S rRNA, ITS-1 and ITS-2 phylogenies. Secondary structure prediction revealed that these four clades differ in hemi-compensatory base changes (HCBCs) in paired positions of the ITS-2, suggesting their inability to interbreed. Reproductively isolated C. neogracilis genotypes can thus co-occur in summer phytoplankton communities in the Beaufort Sea. Chaetoceros neogracilis generally occurred as single cells but can also form short colonies. It is phylogenetically distinct from an Antarctic species, erroneously identified in some previous studies as C. neogracilis but named here as Chaetoceros sp. This work provides taxonomically validated sequences for 20 Arctic diatom taxa, which will facilitate future metabarcoding studies on phytoplankton in this region. |
format |
Article in Journal/Newspaper |
author |
Balzano, Sergio Percopo, Isabella Siano, Raffaele Gourvil, Priscillia Chanoine, Melanie Marie, Dominique Vaulot, Daniel Sarno, Diana |
author_facet |
Balzano, Sergio Percopo, Isabella Siano, Raffaele Gourvil, Priscillia Chanoine, Melanie Marie, Dominique Vaulot, Daniel Sarno, Diana |
author_sort |
Balzano, Sergio |
title |
Morphological and genetic diversity of Beaufort Sea diatoms with high contributions from the Chaetoceros neogracilis species complex |
title_short |
Morphological and genetic diversity of Beaufort Sea diatoms with high contributions from the Chaetoceros neogracilis species complex |
title_full |
Morphological and genetic diversity of Beaufort Sea diatoms with high contributions from the Chaetoceros neogracilis species complex |
title_fullStr |
Morphological and genetic diversity of Beaufort Sea diatoms with high contributions from the Chaetoceros neogracilis species complex |
title_full_unstemmed |
Morphological and genetic diversity of Beaufort Sea diatoms with high contributions from the Chaetoceros neogracilis species complex |
title_sort |
morphological and genetic diversity of beaufort sea diatoms with high contributions from the chaetoceros neogracilis species complex |
publisher |
Wiley |
publishDate |
2017 |
url |
https://archimer.ifremer.fr/doc/00356/46718/46584.pdf https://doi.org/10.1111/jpy.12489 https://archimer.ifremer.fr/doc/00356/46718/ |
geographic |
Antarctic Arctic |
geographic_facet |
Antarctic Arctic |
genre |
Antarc* Antarctic Arctic Beaufort Sea Phytoplankton |
genre_facet |
Antarc* Antarctic Arctic Beaufort Sea Phytoplankton |
op_source |
Journal Of Phycology (0022-3646) (Wiley), 2017-02 , Vol. 53 , N. 1 , P. 161-187 |
op_relation |
info:eu-repo/grantAgreement/EC/FP7/227799/EU//ASSEMBLE info:eu-repo/grantAgreement/EC/FP7/311975/EU//MACUMBA https://archimer.ifremer.fr/doc/00356/46718/46584.pdf doi:10.1111/jpy.12489 https://archimer.ifremer.fr/doc/00356/46718/ |
op_rights |
2016 Phycological Society of America info:eu-repo/semantics/openAccess restricted use |
op_doi |
https://doi.org/10.1111/jpy.12489 |
container_title |
Journal of Phycology |
container_volume |
53 |
container_issue |
1 |
container_start_page |
161 |
op_container_end_page |
187 |
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1766254157790969856 |