Distinct immune responses of juvenile and adult oysters (Crassostrea gigas) to viral and bacterial infections
Since 2008, massive mortality events of Pacific oysters (Crassostrea gigas) have been reported worldwide and these disease events are often associated with Ostreid herpesvirus type 1 (OsHV-1). Epidemiological field studies have also reported oyster age and other pathogens of the Vibrio genus are con...
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Biomed Central Ltd
2016
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Online Access: | https://archimer.ifremer.fr/doc/00348/45875/45575.pdf https://archimer.ifremer.fr/doc/00348/45875/45576.csv https://archimer.ifremer.fr/doc/00348/45875/45577.csv https://doi.org/10.1186/s13567-016-0356-7 https://archimer.ifremer.fr/doc/00348/45875/ |
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ftarchimer:oai:archimer.ifremer.fr:45875 2023-05-15T15:58:07+02:00 Distinct immune responses of juvenile and adult oysters (Crassostrea gigas) to viral and bacterial infections Green, Timothy J. Vergnes, Agnes Montagnani, Caroline De Lorgeril, Julien 2016-07 application/pdf https://archimer.ifremer.fr/doc/00348/45875/45575.pdf https://archimer.ifremer.fr/doc/00348/45875/45576.csv https://archimer.ifremer.fr/doc/00348/45875/45577.csv https://doi.org/10.1186/s13567-016-0356-7 https://archimer.ifremer.fr/doc/00348/45875/ eng eng Biomed Central Ltd https://archimer.ifremer.fr/doc/00348/45875/45575.pdf https://archimer.ifremer.fr/doc/00348/45875/45576.csv https://archimer.ifremer.fr/doc/00348/45875/45577.csv doi:10.1186/s13567-016-0356-7 https://archimer.ifremer.fr/doc/00348/45875/ 2016 The Author(s). This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication. info:eu-repo/semantics/openAccess restricted use PDM CC-BY Veterinary Research (0928-4249) (Biomed Central Ltd), 2016-07 , Vol. 47 , N. 72 , P. 1-11 text Publication info:eu-repo/semantics/article 2016 ftarchimer https://doi.org/10.1186/s13567-016-0356-7 2021-09-23T20:28:42Z Since 2008, massive mortality events of Pacific oysters (Crassostrea gigas) have been reported worldwide and these disease events are often associated with Ostreid herpesvirus type 1 (OsHV-1). Epidemiological field studies have also reported oyster age and other pathogens of the Vibrio genus are contributing factors to this syndrome. We undertook a controlled laboratory experiment to simultaneously investigate survival and immunological response of juvenile and adult C. gigas at different time-points post-infection with OsHV-1, Vibrio tasmaniensis LGP32 and V. aestuarianus. Our data corroborates epidemiological studies that juveniles are more susceptible to OsHV-1, whereas adults are more susceptible to Vibrio. We measured the expression of 102 immune-genes by high-throughput RT-qPCR, which revealed oysters have different transcriptional responses to OsHV-1 and Vibrio. The transcriptional response in the early stages of OsHV-1 infection involved genes related to apoptosis and the interferon-pathway. Transcriptional response to Vibrio infection involved antimicrobial peptides, heat shock proteins and galectins. Interestingly, oysters in the later stages of OsHV-1 infection had a transcriptional response that resembled an antibacterial response, which is suggestive of the oyster's microbiome causing secondary infections (dysbiosis-driven pathology). This study provides molecular evidence that oysters can mount distinct immune response to viral and bacterial pathogens and these responses differ depending on the age of the host. Article in Journal/Newspaper Crassostrea gigas Archimer (Archive Institutionnelle de l'Ifremer - Institut français de recherche pour l'exploitation de la mer) Pacific Veterinary Research 47 1 |
institution |
Open Polar |
collection |
Archimer (Archive Institutionnelle de l'Ifremer - Institut français de recherche pour l'exploitation de la mer) |
op_collection_id |
ftarchimer |
language |
English |
description |
Since 2008, massive mortality events of Pacific oysters (Crassostrea gigas) have been reported worldwide and these disease events are often associated with Ostreid herpesvirus type 1 (OsHV-1). Epidemiological field studies have also reported oyster age and other pathogens of the Vibrio genus are contributing factors to this syndrome. We undertook a controlled laboratory experiment to simultaneously investigate survival and immunological response of juvenile and adult C. gigas at different time-points post-infection with OsHV-1, Vibrio tasmaniensis LGP32 and V. aestuarianus. Our data corroborates epidemiological studies that juveniles are more susceptible to OsHV-1, whereas adults are more susceptible to Vibrio. We measured the expression of 102 immune-genes by high-throughput RT-qPCR, which revealed oysters have different transcriptional responses to OsHV-1 and Vibrio. The transcriptional response in the early stages of OsHV-1 infection involved genes related to apoptosis and the interferon-pathway. Transcriptional response to Vibrio infection involved antimicrobial peptides, heat shock proteins and galectins. Interestingly, oysters in the later stages of OsHV-1 infection had a transcriptional response that resembled an antibacterial response, which is suggestive of the oyster's microbiome causing secondary infections (dysbiosis-driven pathology). This study provides molecular evidence that oysters can mount distinct immune response to viral and bacterial pathogens and these responses differ depending on the age of the host. |
format |
Article in Journal/Newspaper |
author |
Green, Timothy J. Vergnes, Agnes Montagnani, Caroline De Lorgeril, Julien |
spellingShingle |
Green, Timothy J. Vergnes, Agnes Montagnani, Caroline De Lorgeril, Julien Distinct immune responses of juvenile and adult oysters (Crassostrea gigas) to viral and bacterial infections |
author_facet |
Green, Timothy J. Vergnes, Agnes Montagnani, Caroline De Lorgeril, Julien |
author_sort |
Green, Timothy J. |
title |
Distinct immune responses of juvenile and adult oysters (Crassostrea gigas) to viral and bacterial infections |
title_short |
Distinct immune responses of juvenile and adult oysters (Crassostrea gigas) to viral and bacterial infections |
title_full |
Distinct immune responses of juvenile and adult oysters (Crassostrea gigas) to viral and bacterial infections |
title_fullStr |
Distinct immune responses of juvenile and adult oysters (Crassostrea gigas) to viral and bacterial infections |
title_full_unstemmed |
Distinct immune responses of juvenile and adult oysters (Crassostrea gigas) to viral and bacterial infections |
title_sort |
distinct immune responses of juvenile and adult oysters (crassostrea gigas) to viral and bacterial infections |
publisher |
Biomed Central Ltd |
publishDate |
2016 |
url |
https://archimer.ifremer.fr/doc/00348/45875/45575.pdf https://archimer.ifremer.fr/doc/00348/45875/45576.csv https://archimer.ifremer.fr/doc/00348/45875/45577.csv https://doi.org/10.1186/s13567-016-0356-7 https://archimer.ifremer.fr/doc/00348/45875/ |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas |
genre_facet |
Crassostrea gigas |
op_source |
Veterinary Research (0928-4249) (Biomed Central Ltd), 2016-07 , Vol. 47 , N. 72 , P. 1-11 |
op_relation |
https://archimer.ifremer.fr/doc/00348/45875/45575.pdf https://archimer.ifremer.fr/doc/00348/45875/45576.csv https://archimer.ifremer.fr/doc/00348/45875/45577.csv doi:10.1186/s13567-016-0356-7 https://archimer.ifremer.fr/doc/00348/45875/ |
op_rights |
2016 The Author(s). This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication. info:eu-repo/semantics/openAccess restricted use |
op_rightsnorm |
PDM CC-BY |
op_doi |
https://doi.org/10.1186/s13567-016-0356-7 |
container_title |
Veterinary Research |
container_volume |
47 |
container_issue |
1 |
_version_ |
1766393838986854400 |