Transcriptional changes in Crassostrea gigas oyster spat following a parental exposure to the herbicide diuron

The Pacific oyster Crassostrea gigas is the main oyster species produced in the world, and a key coastal economic resource in France. High mortalities affect Pacific oysters since 2008 in France and Europe. Their origins have been attributed to a combination of biotic and abiotic factors, underlinin...

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Published in:Aquatic Toxicology
Main Authors: Rondon, R., Akcha, Farida, Alonso, P., Menard, Dominique, Rouxel, Julien, Montagnani, Caroline, Mitta, Guillaume, Cosseau, C., Grunau, C.
Format: Article in Journal/Newspaper
Language:English
Published: Elsevier Science Bv 2016
Subjects:
Online Access:https://archimer.ifremer.fr/doc/00318/42938/42438.pdf
https://doi.org/10.1016/j.aquatox.2016.03.007
https://archimer.ifremer.fr/doc/00318/42938/
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spelling ftarchimer:oai:archimer.ifremer.fr:42938 2023-05-15T15:58:27+02:00 Transcriptional changes in Crassostrea gigas oyster spat following a parental exposure to the herbicide diuron Rondon, R. Akcha, Farida Alonso, P. Menard, Dominique Rouxel, Julien Montagnani, Caroline Mitta, Guillaume Cosseau, C. Grunau, C. 2016-06 application/pdf https://archimer.ifremer.fr/doc/00318/42938/42438.pdf https://doi.org/10.1016/j.aquatox.2016.03.007 https://archimer.ifremer.fr/doc/00318/42938/ eng eng Elsevier Science Bv https://archimer.ifremer.fr/doc/00318/42938/42438.pdf doi:10.1016/j.aquatox.2016.03.007 https://archimer.ifremer.fr/doc/00318/42938/ 2016 Elsevier B.V. All rights reserved info:eu-repo/semantics/openAccess restricted use Aquatic Toxicology (0166-445X) (Elsevier Science Bv), 2016-06 , Vol. 175 , P. 47-55 Mollusk Oyster Diuron Transcriptomic Ecotoxicology text Publication info:eu-repo/semantics/article 2016 ftarchimer https://doi.org/10.1016/j.aquatox.2016.03.007 2021-09-23T20:27:41Z The Pacific oyster Crassostrea gigas is the main oyster species produced in the world, and a key coastal economic resource in France. High mortalities affect Pacific oysters since 2008 in France and Europe. Their origins have been attributed to a combination of biotic and abiotic factors, underlining the importance of environment quality. The impact of water pollution has been pointed out and one of the pollutants, the genotoxic herbicide diuron, occurs at high concentrations all along the French coasts. Previous work has revealed that a parental exposure to diuron had a strong impact on hatching rates and offspring development even if spats were not exposed to diuron themselves. In this study, we explored for the first time the transcriptional changes occurring in oyster spats (non exposed) originating from genitors exposed to an environmentally relevant concentration of diuron during gametogenesis using the RNAseq methodology. We identified a transcriptomic remodeling revealing an effect of the herbicide. Different molecular pathways involved in energy production, translation and cell proliferation are particularly disturbed. This analysis revealed modulated candidate genes putatively involved in response to oxidative stress and mitochondrial damage in offspring of genitors exposed to diuron. Complementary measures of the activity of enzymes involved in these latter processes corroborate the results obtained at the transcriptomic level. In addition, our results suggested an increase in energy production and mitotic activity in 5-month-spats from diuron-exposed genitors. These results could correspond to a "catch-up growth" phenomenon allowing the spats from diuron-exposed genitors, which displayed a growth delay at 3 months, to gain a normal size when they reach the age of 6 months. These results indicate that exposure to a concentration of diuron that is frequently encountered in the field during the oyster’s gametogenesis stage can impact the next generation and may result in fitness disturbance. Article in Journal/Newspaper Crassostrea gigas Pacific oyster Archimer (Archive Institutionnelle de l'Ifremer - Institut français de recherche pour l'exploitation de la mer) Pacific Aquatic Toxicology 175 47 55
institution Open Polar
collection Archimer (Archive Institutionnelle de l'Ifremer - Institut français de recherche pour l'exploitation de la mer)
op_collection_id ftarchimer
language English
topic Mollusk
Oyster
Diuron
Transcriptomic
Ecotoxicology
spellingShingle Mollusk
Oyster
Diuron
Transcriptomic
Ecotoxicology
Rondon, R.
Akcha, Farida
Alonso, P.
Menard, Dominique
Rouxel, Julien
Montagnani, Caroline
Mitta, Guillaume
Cosseau, C.
Grunau, C.
Transcriptional changes in Crassostrea gigas oyster spat following a parental exposure to the herbicide diuron
topic_facet Mollusk
Oyster
Diuron
Transcriptomic
Ecotoxicology
description The Pacific oyster Crassostrea gigas is the main oyster species produced in the world, and a key coastal economic resource in France. High mortalities affect Pacific oysters since 2008 in France and Europe. Their origins have been attributed to a combination of biotic and abiotic factors, underlining the importance of environment quality. The impact of water pollution has been pointed out and one of the pollutants, the genotoxic herbicide diuron, occurs at high concentrations all along the French coasts. Previous work has revealed that a parental exposure to diuron had a strong impact on hatching rates and offspring development even if spats were not exposed to diuron themselves. In this study, we explored for the first time the transcriptional changes occurring in oyster spats (non exposed) originating from genitors exposed to an environmentally relevant concentration of diuron during gametogenesis using the RNAseq methodology. We identified a transcriptomic remodeling revealing an effect of the herbicide. Different molecular pathways involved in energy production, translation and cell proliferation are particularly disturbed. This analysis revealed modulated candidate genes putatively involved in response to oxidative stress and mitochondrial damage in offspring of genitors exposed to diuron. Complementary measures of the activity of enzymes involved in these latter processes corroborate the results obtained at the transcriptomic level. In addition, our results suggested an increase in energy production and mitotic activity in 5-month-spats from diuron-exposed genitors. These results could correspond to a "catch-up growth" phenomenon allowing the spats from diuron-exposed genitors, which displayed a growth delay at 3 months, to gain a normal size when they reach the age of 6 months. These results indicate that exposure to a concentration of diuron that is frequently encountered in the field during the oyster’s gametogenesis stage can impact the next generation and may result in fitness disturbance.
format Article in Journal/Newspaper
author Rondon, R.
Akcha, Farida
Alonso, P.
Menard, Dominique
Rouxel, Julien
Montagnani, Caroline
Mitta, Guillaume
Cosseau, C.
Grunau, C.
author_facet Rondon, R.
Akcha, Farida
Alonso, P.
Menard, Dominique
Rouxel, Julien
Montagnani, Caroline
Mitta, Guillaume
Cosseau, C.
Grunau, C.
author_sort Rondon, R.
title Transcriptional changes in Crassostrea gigas oyster spat following a parental exposure to the herbicide diuron
title_short Transcriptional changes in Crassostrea gigas oyster spat following a parental exposure to the herbicide diuron
title_full Transcriptional changes in Crassostrea gigas oyster spat following a parental exposure to the herbicide diuron
title_fullStr Transcriptional changes in Crassostrea gigas oyster spat following a parental exposure to the herbicide diuron
title_full_unstemmed Transcriptional changes in Crassostrea gigas oyster spat following a parental exposure to the herbicide diuron
title_sort transcriptional changes in crassostrea gigas oyster spat following a parental exposure to the herbicide diuron
publisher Elsevier Science Bv
publishDate 2016
url https://archimer.ifremer.fr/doc/00318/42938/42438.pdf
https://doi.org/10.1016/j.aquatox.2016.03.007
https://archimer.ifremer.fr/doc/00318/42938/
geographic Pacific
geographic_facet Pacific
genre Crassostrea gigas
Pacific oyster
genre_facet Crassostrea gigas
Pacific oyster
op_source Aquatic Toxicology (0166-445X) (Elsevier Science Bv), 2016-06 , Vol. 175 , P. 47-55
op_relation https://archimer.ifremer.fr/doc/00318/42938/42438.pdf
doi:10.1016/j.aquatox.2016.03.007
https://archimer.ifremer.fr/doc/00318/42938/
op_rights 2016 Elsevier B.V. All rights reserved
info:eu-repo/semantics/openAccess
restricted use
op_doi https://doi.org/10.1016/j.aquatox.2016.03.007
container_title Aquatic Toxicology
container_volume 175
container_start_page 47
op_container_end_page 55
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