Transcriptional changes in Crassostrea gigas oyster spat following a parental exposure to the herbicide diuron
The Pacific oyster Crassostrea gigas is the main oyster species produced in the world, and a key coastal economic resource in France. High mortalities affect Pacific oysters since 2008 in France and Europe. Their origins have been attributed to a combination of biotic and abiotic factors, underlinin...
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Elsevier Science Bv
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Online Access: | https://archimer.ifremer.fr/doc/00318/42938/42438.pdf https://doi.org/10.1016/j.aquatox.2016.03.007 https://archimer.ifremer.fr/doc/00318/42938/ |
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ftarchimer:oai:archimer.ifremer.fr:42938 2023-05-15T15:58:27+02:00 Transcriptional changes in Crassostrea gigas oyster spat following a parental exposure to the herbicide diuron Rondon, R. Akcha, Farida Alonso, P. Menard, Dominique Rouxel, Julien Montagnani, Caroline Mitta, Guillaume Cosseau, C. Grunau, C. 2016-06 application/pdf https://archimer.ifremer.fr/doc/00318/42938/42438.pdf https://doi.org/10.1016/j.aquatox.2016.03.007 https://archimer.ifremer.fr/doc/00318/42938/ eng eng Elsevier Science Bv https://archimer.ifremer.fr/doc/00318/42938/42438.pdf doi:10.1016/j.aquatox.2016.03.007 https://archimer.ifremer.fr/doc/00318/42938/ 2016 Elsevier B.V. All rights reserved info:eu-repo/semantics/openAccess restricted use Aquatic Toxicology (0166-445X) (Elsevier Science Bv), 2016-06 , Vol. 175 , P. 47-55 Mollusk Oyster Diuron Transcriptomic Ecotoxicology text Publication info:eu-repo/semantics/article 2016 ftarchimer https://doi.org/10.1016/j.aquatox.2016.03.007 2021-09-23T20:27:41Z The Pacific oyster Crassostrea gigas is the main oyster species produced in the world, and a key coastal economic resource in France. High mortalities affect Pacific oysters since 2008 in France and Europe. Their origins have been attributed to a combination of biotic and abiotic factors, underlining the importance of environment quality. The impact of water pollution has been pointed out and one of the pollutants, the genotoxic herbicide diuron, occurs at high concentrations all along the French coasts. Previous work has revealed that a parental exposure to diuron had a strong impact on hatching rates and offspring development even if spats were not exposed to diuron themselves. In this study, we explored for the first time the transcriptional changes occurring in oyster spats (non exposed) originating from genitors exposed to an environmentally relevant concentration of diuron during gametogenesis using the RNAseq methodology. We identified a transcriptomic remodeling revealing an effect of the herbicide. Different molecular pathways involved in energy production, translation and cell proliferation are particularly disturbed. This analysis revealed modulated candidate genes putatively involved in response to oxidative stress and mitochondrial damage in offspring of genitors exposed to diuron. Complementary measures of the activity of enzymes involved in these latter processes corroborate the results obtained at the transcriptomic level. In addition, our results suggested an increase in energy production and mitotic activity in 5-month-spats from diuron-exposed genitors. These results could correspond to a "catch-up growth" phenomenon allowing the spats from diuron-exposed genitors, which displayed a growth delay at 3 months, to gain a normal size when they reach the age of 6 months. These results indicate that exposure to a concentration of diuron that is frequently encountered in the field during the oyster’s gametogenesis stage can impact the next generation and may result in fitness disturbance. Article in Journal/Newspaper Crassostrea gigas Pacific oyster Archimer (Archive Institutionnelle de l'Ifremer - Institut français de recherche pour l'exploitation de la mer) Pacific Aquatic Toxicology 175 47 55 |
institution |
Open Polar |
collection |
Archimer (Archive Institutionnelle de l'Ifremer - Institut français de recherche pour l'exploitation de la mer) |
op_collection_id |
ftarchimer |
language |
English |
topic |
Mollusk Oyster Diuron Transcriptomic Ecotoxicology |
spellingShingle |
Mollusk Oyster Diuron Transcriptomic Ecotoxicology Rondon, R. Akcha, Farida Alonso, P. Menard, Dominique Rouxel, Julien Montagnani, Caroline Mitta, Guillaume Cosseau, C. Grunau, C. Transcriptional changes in Crassostrea gigas oyster spat following a parental exposure to the herbicide diuron |
topic_facet |
Mollusk Oyster Diuron Transcriptomic Ecotoxicology |
description |
The Pacific oyster Crassostrea gigas is the main oyster species produced in the world, and a key coastal economic resource in France. High mortalities affect Pacific oysters since 2008 in France and Europe. Their origins have been attributed to a combination of biotic and abiotic factors, underlining the importance of environment quality. The impact of water pollution has been pointed out and one of the pollutants, the genotoxic herbicide diuron, occurs at high concentrations all along the French coasts. Previous work has revealed that a parental exposure to diuron had a strong impact on hatching rates and offspring development even if spats were not exposed to diuron themselves. In this study, we explored for the first time the transcriptional changes occurring in oyster spats (non exposed) originating from genitors exposed to an environmentally relevant concentration of diuron during gametogenesis using the RNAseq methodology. We identified a transcriptomic remodeling revealing an effect of the herbicide. Different molecular pathways involved in energy production, translation and cell proliferation are particularly disturbed. This analysis revealed modulated candidate genes putatively involved in response to oxidative stress and mitochondrial damage in offspring of genitors exposed to diuron. Complementary measures of the activity of enzymes involved in these latter processes corroborate the results obtained at the transcriptomic level. In addition, our results suggested an increase in energy production and mitotic activity in 5-month-spats from diuron-exposed genitors. These results could correspond to a "catch-up growth" phenomenon allowing the spats from diuron-exposed genitors, which displayed a growth delay at 3 months, to gain a normal size when they reach the age of 6 months. These results indicate that exposure to a concentration of diuron that is frequently encountered in the field during the oyster’s gametogenesis stage can impact the next generation and may result in fitness disturbance. |
format |
Article in Journal/Newspaper |
author |
Rondon, R. Akcha, Farida Alonso, P. Menard, Dominique Rouxel, Julien Montagnani, Caroline Mitta, Guillaume Cosseau, C. Grunau, C. |
author_facet |
Rondon, R. Akcha, Farida Alonso, P. Menard, Dominique Rouxel, Julien Montagnani, Caroline Mitta, Guillaume Cosseau, C. Grunau, C. |
author_sort |
Rondon, R. |
title |
Transcriptional changes in Crassostrea gigas oyster spat following a parental exposure to the herbicide diuron |
title_short |
Transcriptional changes in Crassostrea gigas oyster spat following a parental exposure to the herbicide diuron |
title_full |
Transcriptional changes in Crassostrea gigas oyster spat following a parental exposure to the herbicide diuron |
title_fullStr |
Transcriptional changes in Crassostrea gigas oyster spat following a parental exposure to the herbicide diuron |
title_full_unstemmed |
Transcriptional changes in Crassostrea gigas oyster spat following a parental exposure to the herbicide diuron |
title_sort |
transcriptional changes in crassostrea gigas oyster spat following a parental exposure to the herbicide diuron |
publisher |
Elsevier Science Bv |
publishDate |
2016 |
url |
https://archimer.ifremer.fr/doc/00318/42938/42438.pdf https://doi.org/10.1016/j.aquatox.2016.03.007 https://archimer.ifremer.fr/doc/00318/42938/ |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
Aquatic Toxicology (0166-445X) (Elsevier Science Bv), 2016-06 , Vol. 175 , P. 47-55 |
op_relation |
https://archimer.ifremer.fr/doc/00318/42938/42438.pdf doi:10.1016/j.aquatox.2016.03.007 https://archimer.ifremer.fr/doc/00318/42938/ |
op_rights |
2016 Elsevier B.V. All rights reserved info:eu-repo/semantics/openAccess restricted use |
op_doi |
https://doi.org/10.1016/j.aquatox.2016.03.007 |
container_title |
Aquatic Toxicology |
container_volume |
175 |
container_start_page |
47 |
op_container_end_page |
55 |
_version_ |
1766394189189218304 |