A flow-through rearing system for ecophysiological studies of Pacific oyster Crassostrea gigas larvae
While literature is relatively abundant on adult shellfish, ecophysiological studies at the larval stage are scarce because of both technical difficulties and inadequate methodology. A tool dedicated to provide basic information for larval ecophysiology was accordingly developed. Two steps were foll...
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Elsevier
2008
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Online Access: | https://archimer.ifremer.fr/doc/2008/publication-4167.pdf https://doi.org/10.1016/j.aquaculture.2008.06.016 https://archimer.ifremer.fr/doc/00000/4167/ |
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ftarchimer:oai:archimer.ifremer.fr:4167 2023-05-15T15:57:55+02:00 A flow-through rearing system for ecophysiological studies of Pacific oyster Crassostrea gigas larvae Rico Villa, Benjamin Woerther, Patrice Mingant, Christian Le Piver, David Pouvreau, Stephane Hamon, Michel Robert, Rene 2008-06 application/pdf https://archimer.ifremer.fr/doc/2008/publication-4167.pdf https://doi.org/10.1016/j.aquaculture.2008.06.016 https://archimer.ifremer.fr/doc/00000/4167/ eng eng Elsevier https://archimer.ifremer.fr/doc/2008/publication-4167.pdf doi:10.1016/j.aquaculture.2008.06.016 https://archimer.ifremer.fr/doc/00000/4167/ 2008 Elsevier B.V. All rights reserved info:eu-repo/semantics/openAccess restricted use Aquaculture (0044-8486) (Elsevier), 2008-06 , Vol. 282 , N. 1-4 , P. 54-60 Temperature Ecophysiology Bivalve larvae Crassostrea gigas Flow through text Publication info:eu-repo/semantics/article 2008 ftarchimer https://doi.org/10.1016/j.aquaculture.2008.06.016 2021-09-23T20:15:54Z While literature is relatively abundant on adult shellfish, ecophysiological studies at the larval stage are scarce because of both technical difficulties and inadequate methodology. A tool dedicated to provide basic information for larval ecophysiology was accordingly developed. Two steps were followed: first a flow-through method of Crassostrea gigas larval culture was perfected during a set of experiments in which rearing systems and larval density were assessed. Then a continuous hydrobiological data recorder was adapted with modifications to comply with our experimental aim. SILO (Système d'Instrumentation des Larves en flux Ouvert) allowed the successive acquisition of hydrobiological parameters in ten 150 l larval tanks in which larvae were reared using flow-through techniques under controlled environmental conditions. An automated system enabled sequential measurement of hydrobiological parameters from one larval culture to the next. A chamber system contained several probes for measurement of temperature, salinity, pH and fluorescence. The electronic system allowed real time acquisition, storage and transfer of data. SILO was successful as a larval rearing device, reducing larval disturbance that could result from transfer and handling for measurement. It was also efficient as a tool to provide basic information for larval ecophysiology research. The effect of temperature (17, 22, 25, 27, 32°C) on growth and ingestion of microalgae was studied to test SILO. Maximum growth occurred at 27 °C (16 ± 2 μm d-1) and 32 °C (15 ± 3 μm d-1) whereas larvae reared at 22 and 25°C showed lower development (8.6 ± 3.1 and 11.7 ± 2.5 μm d-1, respectively). Moreover, metamorphosis exhibited no significant difference at 27 or 32 °C with 87.5 ± 7.1 and 85.9 ± 9.6% respectively, while at 22 or 25 °C lower metamorphosis was recorded with 55.2 ± 8.3 and 57.6 ± 9.6%, respectively. The lowest temperature (17 °C) strongly inhibited ingestion whereas the highest temperature (32 °C) stimulated maximum feeding activity over the whole larval rearing period. Article in Journal/Newspaper Crassostrea gigas Pacific oyster Archimer (Archive Institutionnelle de l'Ifremer - Institut français de recherche pour l'exploitation de la mer) Pacific Aquaculture 282 1-4 54 60 |
institution |
Open Polar |
collection |
Archimer (Archive Institutionnelle de l'Ifremer - Institut français de recherche pour l'exploitation de la mer) |
op_collection_id |
ftarchimer |
language |
English |
topic |
Temperature Ecophysiology Bivalve larvae Crassostrea gigas Flow through |
spellingShingle |
Temperature Ecophysiology Bivalve larvae Crassostrea gigas Flow through Rico Villa, Benjamin Woerther, Patrice Mingant, Christian Le Piver, David Pouvreau, Stephane Hamon, Michel Robert, Rene A flow-through rearing system for ecophysiological studies of Pacific oyster Crassostrea gigas larvae |
topic_facet |
Temperature Ecophysiology Bivalve larvae Crassostrea gigas Flow through |
description |
While literature is relatively abundant on adult shellfish, ecophysiological studies at the larval stage are scarce because of both technical difficulties and inadequate methodology. A tool dedicated to provide basic information for larval ecophysiology was accordingly developed. Two steps were followed: first a flow-through method of Crassostrea gigas larval culture was perfected during a set of experiments in which rearing systems and larval density were assessed. Then a continuous hydrobiological data recorder was adapted with modifications to comply with our experimental aim. SILO (Système d'Instrumentation des Larves en flux Ouvert) allowed the successive acquisition of hydrobiological parameters in ten 150 l larval tanks in which larvae were reared using flow-through techniques under controlled environmental conditions. An automated system enabled sequential measurement of hydrobiological parameters from one larval culture to the next. A chamber system contained several probes for measurement of temperature, salinity, pH and fluorescence. The electronic system allowed real time acquisition, storage and transfer of data. SILO was successful as a larval rearing device, reducing larval disturbance that could result from transfer and handling for measurement. It was also efficient as a tool to provide basic information for larval ecophysiology research. The effect of temperature (17, 22, 25, 27, 32°C) on growth and ingestion of microalgae was studied to test SILO. Maximum growth occurred at 27 °C (16 ± 2 μm d-1) and 32 °C (15 ± 3 μm d-1) whereas larvae reared at 22 and 25°C showed lower development (8.6 ± 3.1 and 11.7 ± 2.5 μm d-1, respectively). Moreover, metamorphosis exhibited no significant difference at 27 or 32 °C with 87.5 ± 7.1 and 85.9 ± 9.6% respectively, while at 22 or 25 °C lower metamorphosis was recorded with 55.2 ± 8.3 and 57.6 ± 9.6%, respectively. The lowest temperature (17 °C) strongly inhibited ingestion whereas the highest temperature (32 °C) stimulated maximum feeding activity over the whole larval rearing period. |
format |
Article in Journal/Newspaper |
author |
Rico Villa, Benjamin Woerther, Patrice Mingant, Christian Le Piver, David Pouvreau, Stephane Hamon, Michel Robert, Rene |
author_facet |
Rico Villa, Benjamin Woerther, Patrice Mingant, Christian Le Piver, David Pouvreau, Stephane Hamon, Michel Robert, Rene |
author_sort |
Rico Villa, Benjamin |
title |
A flow-through rearing system for ecophysiological studies of Pacific oyster Crassostrea gigas larvae |
title_short |
A flow-through rearing system for ecophysiological studies of Pacific oyster Crassostrea gigas larvae |
title_full |
A flow-through rearing system for ecophysiological studies of Pacific oyster Crassostrea gigas larvae |
title_fullStr |
A flow-through rearing system for ecophysiological studies of Pacific oyster Crassostrea gigas larvae |
title_full_unstemmed |
A flow-through rearing system for ecophysiological studies of Pacific oyster Crassostrea gigas larvae |
title_sort |
flow-through rearing system for ecophysiological studies of pacific oyster crassostrea gigas larvae |
publisher |
Elsevier |
publishDate |
2008 |
url |
https://archimer.ifremer.fr/doc/2008/publication-4167.pdf https://doi.org/10.1016/j.aquaculture.2008.06.016 https://archimer.ifremer.fr/doc/00000/4167/ |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
Aquaculture (0044-8486) (Elsevier), 2008-06 , Vol. 282 , N. 1-4 , P. 54-60 |
op_relation |
https://archimer.ifremer.fr/doc/2008/publication-4167.pdf doi:10.1016/j.aquaculture.2008.06.016 https://archimer.ifremer.fr/doc/00000/4167/ |
op_rights |
2008 Elsevier B.V. All rights reserved info:eu-repo/semantics/openAccess restricted use |
op_doi |
https://doi.org/10.1016/j.aquaculture.2008.06.016 |
container_title |
Aquaculture |
container_volume |
282 |
container_issue |
1-4 |
container_start_page |
54 |
op_container_end_page |
60 |
_version_ |
1766393624208080896 |