Otolith growth in trout Oncorhynchus mykiss: supply of Ca2+ and Sr2+ to the saccular endolymph
Kinetic and pharmacological characteristics of Ca2+ fluxes across the saccular epithelium of trout were studied using a perfused isolated inner ear. Ca-45(2+) influx from the Ringer solution to the endolymph was 3-4 nmoles h(-1) mu(-1) endolymph, which corresponds to a global turnover rate of the en...
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ftarchimer:oai:archimer.ifremer.fr:416 2023-05-15T18:41:17+02:00 Otolith growth in trout Oncorhynchus mykiss: supply of Ca2+ and Sr2+ to the saccular endolymph Payan, P Borelli, G Priouzeau, F De Pontual, Helene Boeuf, G Mayer Gostan, N 2002-09 application/pdf https://archimer.ifremer.fr/doc/2002/publication-416.pdf https://archimer.ifremer.fr/doc/00000/416/ eng eng Company of Biologists https://archimer.ifremer.fr/doc/2002/publication-416.pdf https://archimer.ifremer.fr/doc/00000/416/ 2002 The Company of Biologists Limited info:eu-repo/semantics/openAccess restricted use Journal of Experimental Biology (0022-0949) (Company of Biologists), 2002-09 , Vol. 205 , N. 17 , P. 2687-2695 Inner ear Perfusion Otolith Endolymph Flux Calcium Oncorhynchus mykiss Trout text Publication info:eu-repo/semantics/article 2002 ftarchimer 2021-09-23T20:13:07Z Kinetic and pharmacological characteristics of Ca2+ fluxes across the saccular epithelium of trout were studied using a perfused isolated inner ear. Ca-45(2+) influx from the Ringer solution to the endolymph was 3-4 nmoles h(-1) mu(-1) endolymph, which corresponds to a global turnover rate of the endolymph calcium of 200% h(-1). Ca2+ entry into the proximal endolymph was faster than into the distal fluid. Net Ca2+ movement across the saccular epithelium depended on the direction and intensity of the chemical gradient of calcium between the Ringer solution and the endolymph. Increasing the calcium concentration in the Ringer solution up to 4.4 mmol l(-1) provoked an accumulation of Ca2+ in both proximal and distal endolymphs, and equilibrium was reached about 30 min after the beginning of perfusion. Perfusion with calcium-free Ringer partially emptied the proximal compartment of calcium, whereas the calcium levels in the distal endolymph did not vary during 70 min of perfusion. Verapamil (10(-5) mol l(-1)) and cyanide (CN, 10(-3) mol l(-1)) did not modify the accumulation of Ca2+ within the endolymph in the presence of a favourable calcium chemical gradient. Furthermore the relationship between Ca2+ net fluxes and the chemical calcium gradient across the saccular epithelium was linear, indicating a passive diffusional mechanism via a paracellular pathway. Similar relationships were found for Sr2+ fluxes across the saccular epithelium in the presence of positive chemical gradients (1, 2 and 4 mmol l(-1) Sr2+). In vivo experiments in which trout were intraperitoneously injected with CaCl2 solution confirmed the tight relationship between the calcium levels in plasma and endolymph (both proximal and distal). Sampling proximal and distal endolymphs in trout and turbot saccules revealed a decreasing proximo-distal calcium gradient in endolymph of both fish species. The present results strongly suggest that the endolymph is supplied with Ca2+ and Sr2+ via a paracellular pathway located in the proximal area of the saccular epithelium. Article in Journal/Newspaper Turbot Archimer (Archive Institutionnelle de l'Ifremer - Institut français de recherche pour l'exploitation de la mer) Ringer ENVELOPE(162.050,162.050,-77.250,-77.250) The Ringer ENVELOPE(161.850,161.850,-77.250,-77.250) |
institution |
Open Polar |
collection |
Archimer (Archive Institutionnelle de l'Ifremer - Institut français de recherche pour l'exploitation de la mer) |
op_collection_id |
ftarchimer |
language |
English |
topic |
Inner ear Perfusion Otolith Endolymph Flux Calcium Oncorhynchus mykiss Trout |
spellingShingle |
Inner ear Perfusion Otolith Endolymph Flux Calcium Oncorhynchus mykiss Trout Payan, P Borelli, G Priouzeau, F De Pontual, Helene Boeuf, G Mayer Gostan, N Otolith growth in trout Oncorhynchus mykiss: supply of Ca2+ and Sr2+ to the saccular endolymph |
topic_facet |
Inner ear Perfusion Otolith Endolymph Flux Calcium Oncorhynchus mykiss Trout |
description |
Kinetic and pharmacological characteristics of Ca2+ fluxes across the saccular epithelium of trout were studied using a perfused isolated inner ear. Ca-45(2+) influx from the Ringer solution to the endolymph was 3-4 nmoles h(-1) mu(-1) endolymph, which corresponds to a global turnover rate of the endolymph calcium of 200% h(-1). Ca2+ entry into the proximal endolymph was faster than into the distal fluid. Net Ca2+ movement across the saccular epithelium depended on the direction and intensity of the chemical gradient of calcium between the Ringer solution and the endolymph. Increasing the calcium concentration in the Ringer solution up to 4.4 mmol l(-1) provoked an accumulation of Ca2+ in both proximal and distal endolymphs, and equilibrium was reached about 30 min after the beginning of perfusion. Perfusion with calcium-free Ringer partially emptied the proximal compartment of calcium, whereas the calcium levels in the distal endolymph did not vary during 70 min of perfusion. Verapamil (10(-5) mol l(-1)) and cyanide (CN, 10(-3) mol l(-1)) did not modify the accumulation of Ca2+ within the endolymph in the presence of a favourable calcium chemical gradient. Furthermore the relationship between Ca2+ net fluxes and the chemical calcium gradient across the saccular epithelium was linear, indicating a passive diffusional mechanism via a paracellular pathway. Similar relationships were found for Sr2+ fluxes across the saccular epithelium in the presence of positive chemical gradients (1, 2 and 4 mmol l(-1) Sr2+). In vivo experiments in which trout were intraperitoneously injected with CaCl2 solution confirmed the tight relationship between the calcium levels in plasma and endolymph (both proximal and distal). Sampling proximal and distal endolymphs in trout and turbot saccules revealed a decreasing proximo-distal calcium gradient in endolymph of both fish species. The present results strongly suggest that the endolymph is supplied with Ca2+ and Sr2+ via a paracellular pathway located in the proximal area of the saccular epithelium. |
format |
Article in Journal/Newspaper |
author |
Payan, P Borelli, G Priouzeau, F De Pontual, Helene Boeuf, G Mayer Gostan, N |
author_facet |
Payan, P Borelli, G Priouzeau, F De Pontual, Helene Boeuf, G Mayer Gostan, N |
author_sort |
Payan, P |
title |
Otolith growth in trout Oncorhynchus mykiss: supply of Ca2+ and Sr2+ to the saccular endolymph |
title_short |
Otolith growth in trout Oncorhynchus mykiss: supply of Ca2+ and Sr2+ to the saccular endolymph |
title_full |
Otolith growth in trout Oncorhynchus mykiss: supply of Ca2+ and Sr2+ to the saccular endolymph |
title_fullStr |
Otolith growth in trout Oncorhynchus mykiss: supply of Ca2+ and Sr2+ to the saccular endolymph |
title_full_unstemmed |
Otolith growth in trout Oncorhynchus mykiss: supply of Ca2+ and Sr2+ to the saccular endolymph |
title_sort |
otolith growth in trout oncorhynchus mykiss: supply of ca2+ and sr2+ to the saccular endolymph |
publisher |
Company of Biologists |
publishDate |
2002 |
url |
https://archimer.ifremer.fr/doc/2002/publication-416.pdf https://archimer.ifremer.fr/doc/00000/416/ |
long_lat |
ENVELOPE(162.050,162.050,-77.250,-77.250) ENVELOPE(161.850,161.850,-77.250,-77.250) |
geographic |
Ringer The Ringer |
geographic_facet |
Ringer The Ringer |
genre |
Turbot |
genre_facet |
Turbot |
op_source |
Journal of Experimental Biology (0022-0949) (Company of Biologists), 2002-09 , Vol. 205 , N. 17 , P. 2687-2695 |
op_relation |
https://archimer.ifremer.fr/doc/2002/publication-416.pdf https://archimer.ifremer.fr/doc/00000/416/ |
op_rights |
2002 The Company of Biologists Limited info:eu-repo/semantics/openAccess restricted use |
_version_ |
1766230788428267520 |