Involvement of Mitochondrial Activity and OXPHOS in ATP Synthesis During the Motility Phase of Spermatozoa in the Pacific Oyster, Crassostrea gigas
In the Pacific oyster, spermatozoa are characterized by a remarkably long movement phase (i.e., over 24 h) sustained by a capacity to maintain intracellular ATP level. To gain information on oxidative phosphorylation (OXPHOS) functionality during the motility phase of Pacific oyster spermatozoa, we...
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Online Access: | https://archimer.ifremer.fr/doc/00303/41376/40666.pdf https://doi.org/10.1095/biolreprod.115.128538 https://archimer.ifremer.fr/doc/00303/41376/ |
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ftarchimer:oai:archimer.ifremer.fr:41376 2023-05-15T15:58:20+02:00 Involvement of Mitochondrial Activity and OXPHOS in ATP Synthesis During the Motility Phase of Spermatozoa in the Pacific Oyster, Crassostrea gigas Boulais, Myrina Soudant, Philippe Le Goic, Nelly Quere, Claudie Boudry, Pierre Suquet, Marc 2015-11 application/pdf https://archimer.ifremer.fr/doc/00303/41376/40666.pdf https://doi.org/10.1095/biolreprod.115.128538 https://archimer.ifremer.fr/doc/00303/41376/ eng eng Soc Study Reproduction https://archimer.ifremer.fr/doc/00303/41376/40666.pdf doi:10.1095/biolreprod.115.128538 https://archimer.ifremer.fr/doc/00303/41376/ 2015 by the Society for the Study of Reproduction, Inc. info:eu-repo/semantics/openAccess restricted use Biology Of Reproduction (0006-3363) (Soc Study Reproduction), 2015-11 , Vol. 93 , N. 5 Crassostrea gigas intracellular ATP content mitochondrial membrane potential oxidative phosphorylation sperm motility text Publication info:eu-repo/semantics/article 2015 ftarchimer https://doi.org/10.1095/biolreprod.115.128538 2021-09-23T20:27:14Z In the Pacific oyster, spermatozoa are characterized by a remarkably long movement phase (i.e., over 24 h) sustained by a capacity to maintain intracellular ATP level. To gain information on oxidative phosphorylation (OXPHOS) functionality during the motility phase of Pacific oyster spermatozoa, we studied 1) changes in spermatozoal mitochondrial activity, that is, mitochondrial membrane potential (MMP), and intracellular ATP content in relation to motion parameters and 2) the involvement of OXPHOS for spermatozoal movement using carbonyl cyanide m-chlorophenyl hydrazone (CCCP). The percentage of motile spermatozoa decreased over a 24 h movement period. MMP increased steadily during the first 9 h of the movement phase and was subsequently maintained at a constant level. Conversely, spermatozoal ATP content decreased steadily during the first 9 h postactivation and was maintained at this level during the following hours of the movement phase. When OXPHOS was decoupled by CCCP, the movement of spermatozoa was maintained 2 h and totally stopped after 4 h of incubation, whereas spermatozoa were still motile in the control after 4 h. Our results suggest that the ATP sustaining flagellar movement of spermatozoa may partially originate from glycolysis or from mobilization of stored ATP or from potential phosphagens during the first 2 h of movement as deduced by the decoupling by CCCP of OXPHOS. However, OXPHOS is required to sustain the long motility phase of Pacific oyster spermatozoa. In addition, spermatozoa may hydrolyze intracellular ATP content during the early part of the movement phase, stimulating mitochondrial activity. This stimulation seems to be involved in sustaining a high ATP level until the end of the motility phase. Article in Journal/Newspaper Crassostrea gigas Pacific oyster Archimer (Archive Institutionnelle de l'Ifremer - Institut français de recherche pour l'exploitation de la mer) Pacific Biology of Reproduction 93 5 |
institution |
Open Polar |
collection |
Archimer (Archive Institutionnelle de l'Ifremer - Institut français de recherche pour l'exploitation de la mer) |
op_collection_id |
ftarchimer |
language |
English |
topic |
Crassostrea gigas intracellular ATP content mitochondrial membrane potential oxidative phosphorylation sperm motility |
spellingShingle |
Crassostrea gigas intracellular ATP content mitochondrial membrane potential oxidative phosphorylation sperm motility Boulais, Myrina Soudant, Philippe Le Goic, Nelly Quere, Claudie Boudry, Pierre Suquet, Marc Involvement of Mitochondrial Activity and OXPHOS in ATP Synthesis During the Motility Phase of Spermatozoa in the Pacific Oyster, Crassostrea gigas |
topic_facet |
Crassostrea gigas intracellular ATP content mitochondrial membrane potential oxidative phosphorylation sperm motility |
description |
In the Pacific oyster, spermatozoa are characterized by a remarkably long movement phase (i.e., over 24 h) sustained by a capacity to maintain intracellular ATP level. To gain information on oxidative phosphorylation (OXPHOS) functionality during the motility phase of Pacific oyster spermatozoa, we studied 1) changes in spermatozoal mitochondrial activity, that is, mitochondrial membrane potential (MMP), and intracellular ATP content in relation to motion parameters and 2) the involvement of OXPHOS for spermatozoal movement using carbonyl cyanide m-chlorophenyl hydrazone (CCCP). The percentage of motile spermatozoa decreased over a 24 h movement period. MMP increased steadily during the first 9 h of the movement phase and was subsequently maintained at a constant level. Conversely, spermatozoal ATP content decreased steadily during the first 9 h postactivation and was maintained at this level during the following hours of the movement phase. When OXPHOS was decoupled by CCCP, the movement of spermatozoa was maintained 2 h and totally stopped after 4 h of incubation, whereas spermatozoa were still motile in the control after 4 h. Our results suggest that the ATP sustaining flagellar movement of spermatozoa may partially originate from glycolysis or from mobilization of stored ATP or from potential phosphagens during the first 2 h of movement as deduced by the decoupling by CCCP of OXPHOS. However, OXPHOS is required to sustain the long motility phase of Pacific oyster spermatozoa. In addition, spermatozoa may hydrolyze intracellular ATP content during the early part of the movement phase, stimulating mitochondrial activity. This stimulation seems to be involved in sustaining a high ATP level until the end of the motility phase. |
format |
Article in Journal/Newspaper |
author |
Boulais, Myrina Soudant, Philippe Le Goic, Nelly Quere, Claudie Boudry, Pierre Suquet, Marc |
author_facet |
Boulais, Myrina Soudant, Philippe Le Goic, Nelly Quere, Claudie Boudry, Pierre Suquet, Marc |
author_sort |
Boulais, Myrina |
title |
Involvement of Mitochondrial Activity and OXPHOS in ATP Synthesis During the Motility Phase of Spermatozoa in the Pacific Oyster, Crassostrea gigas |
title_short |
Involvement of Mitochondrial Activity and OXPHOS in ATP Synthesis During the Motility Phase of Spermatozoa in the Pacific Oyster, Crassostrea gigas |
title_full |
Involvement of Mitochondrial Activity and OXPHOS in ATP Synthesis During the Motility Phase of Spermatozoa in the Pacific Oyster, Crassostrea gigas |
title_fullStr |
Involvement of Mitochondrial Activity and OXPHOS in ATP Synthesis During the Motility Phase of Spermatozoa in the Pacific Oyster, Crassostrea gigas |
title_full_unstemmed |
Involvement of Mitochondrial Activity and OXPHOS in ATP Synthesis During the Motility Phase of Spermatozoa in the Pacific Oyster, Crassostrea gigas |
title_sort |
involvement of mitochondrial activity and oxphos in atp synthesis during the motility phase of spermatozoa in the pacific oyster, crassostrea gigas |
publisher |
Soc Study Reproduction |
publishDate |
2015 |
url |
https://archimer.ifremer.fr/doc/00303/41376/40666.pdf https://doi.org/10.1095/biolreprod.115.128538 https://archimer.ifremer.fr/doc/00303/41376/ |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
Biology Of Reproduction (0006-3363) (Soc Study Reproduction), 2015-11 , Vol. 93 , N. 5 |
op_relation |
https://archimer.ifremer.fr/doc/00303/41376/40666.pdf doi:10.1095/biolreprod.115.128538 https://archimer.ifremer.fr/doc/00303/41376/ |
op_rights |
2015 by the Society for the Study of Reproduction, Inc. info:eu-repo/semantics/openAccess restricted use |
op_doi |
https://doi.org/10.1095/biolreprod.115.128538 |
container_title |
Biology of Reproduction |
container_volume |
93 |
container_issue |
5 |
_version_ |
1766394069876998144 |