In vitro culture of mollusc hemocytes. Functional study of burst respiratory activity and analysis of interactions with protozoan and procaryotic pathogens
The chief importance of hemocytes as immune effectors in molluscs has led to develop researches for in vitro culture. The chemiluminescence technique has been used to estimate the phagocytosis capacity of Crassostrea gigas hemocytes according to different culture media and times. Sea water was used...
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Actes du colloque, 8th International Conference on Invertebrate and Fish tissue culture. Tissue culture association, Comubia : pp 56-64
1991
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ftarchimer:oai:archimer.ifremer.fr:4115 2023-05-15T15:58:57+02:00 In vitro culture of mollusc hemocytes. Functional study of burst respiratory activity and analysis of interactions with protozoan and procaryotic pathogens Boulo, Viviane Hervio, Dominique Morvan, Annie Bachere, Evelyne Mialhe, Eric 1991 application/pdf https://archimer.ifremer.fr/doc/1991/acte-4115.PDF https://archimer.ifremer.fr/doc/00000/4115/ eng eng Actes du colloque, 8th International Conference on Invertebrate and Fish tissue culture. Tissue culture association, Comubia : pp 56-64 https://archimer.ifremer.fr/doc/1991/acte-4115.PDF https://archimer.ifremer.fr/doc/00000/4115/ info:eu-repo/semantics/openAccess restricted use Crassotrea gigas Oyster Bonamia ostreae Pathogens Molluscs Hemocytes text Conference article info:eu-repo/semantics/conferenceObject 1991 ftarchimer 2021-09-23T20:15:54Z The chief importance of hemocytes as immune effectors in molluscs has led to develop researches for in vitro culture. The chemiluminescence technique has been used to estimate the phagocytosis capacity of Crassostrea gigas hemocytes according to different culture media and times. Sea water was used as a basic medium. Additives, such as antibiotics or glucose and buffers, such as Tris or Hepes, were tested as weIl as artificial sea water. Hemocyte chemiluminescence activities were recorded at t=o in relation to the medium composition. The highest values were observed when hemocytes were kept in sea water without any modification. Whichever medium, chemiluminescence activity was substantially decreased when hemocytes were previously kept in vitro for a few hours. Thus, chemiluminescence technique could be a well-adapted method to estimate the functional capacity of mollusc hemocytes and to study medium composition in prospect of long-term molluscan cell cultures. Despite present limitations of short-term hemocyte primary cultures, they were used to study in vitro the interactions between hemocytes and some mollusc-specific pathogens. The hemocyte recognition and entry mechanisms of Bonamia ostreae (Protozoa: Ascetospora), an intrahaemocytic parasite of fIat oyster Ostrea edulis, were investigated. Moreover, it was demonstrated that hemocyte respiratory burst was not triggered during parasite phagocytosis. Similar experiments were performed with St-Jacques scallop (Pecten maximus) hemocytes and a host-specifie gill Rickettsialeslike organism, leading to evidenee some pathogen adaptation for escaping host-immune response. These first in vitro models extrapolated to other pathogens anti-infectious immune response of molluscs. Conference Object Crassostrea gigas Archimer (Archive Institutionnelle de l'Ifremer - Institut français de recherche pour l'exploitation de la mer) |
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Open Polar |
collection |
Archimer (Archive Institutionnelle de l'Ifremer - Institut français de recherche pour l'exploitation de la mer) |
op_collection_id |
ftarchimer |
language |
English |
topic |
Crassotrea gigas Oyster Bonamia ostreae Pathogens Molluscs Hemocytes |
spellingShingle |
Crassotrea gigas Oyster Bonamia ostreae Pathogens Molluscs Hemocytes Boulo, Viviane Hervio, Dominique Morvan, Annie Bachere, Evelyne Mialhe, Eric In vitro culture of mollusc hemocytes. Functional study of burst respiratory activity and analysis of interactions with protozoan and procaryotic pathogens |
topic_facet |
Crassotrea gigas Oyster Bonamia ostreae Pathogens Molluscs Hemocytes |
description |
The chief importance of hemocytes as immune effectors in molluscs has led to develop researches for in vitro culture. The chemiluminescence technique has been used to estimate the phagocytosis capacity of Crassostrea gigas hemocytes according to different culture media and times. Sea water was used as a basic medium. Additives, such as antibiotics or glucose and buffers, such as Tris or Hepes, were tested as weIl as artificial sea water. Hemocyte chemiluminescence activities were recorded at t=o in relation to the medium composition. The highest values were observed when hemocytes were kept in sea water without any modification. Whichever medium, chemiluminescence activity was substantially decreased when hemocytes were previously kept in vitro for a few hours. Thus, chemiluminescence technique could be a well-adapted method to estimate the functional capacity of mollusc hemocytes and to study medium composition in prospect of long-term molluscan cell cultures. Despite present limitations of short-term hemocyte primary cultures, they were used to study in vitro the interactions between hemocytes and some mollusc-specific pathogens. The hemocyte recognition and entry mechanisms of Bonamia ostreae (Protozoa: Ascetospora), an intrahaemocytic parasite of fIat oyster Ostrea edulis, were investigated. Moreover, it was demonstrated that hemocyte respiratory burst was not triggered during parasite phagocytosis. Similar experiments were performed with St-Jacques scallop (Pecten maximus) hemocytes and a host-specifie gill Rickettsialeslike organism, leading to evidenee some pathogen adaptation for escaping host-immune response. These first in vitro models extrapolated to other pathogens anti-infectious immune response of molluscs. |
format |
Conference Object |
author |
Boulo, Viviane Hervio, Dominique Morvan, Annie Bachere, Evelyne Mialhe, Eric |
author_facet |
Boulo, Viviane Hervio, Dominique Morvan, Annie Bachere, Evelyne Mialhe, Eric |
author_sort |
Boulo, Viviane |
title |
In vitro culture of mollusc hemocytes. Functional study of burst respiratory activity and analysis of interactions with protozoan and procaryotic pathogens |
title_short |
In vitro culture of mollusc hemocytes. Functional study of burst respiratory activity and analysis of interactions with protozoan and procaryotic pathogens |
title_full |
In vitro culture of mollusc hemocytes. Functional study of burst respiratory activity and analysis of interactions with protozoan and procaryotic pathogens |
title_fullStr |
In vitro culture of mollusc hemocytes. Functional study of burst respiratory activity and analysis of interactions with protozoan and procaryotic pathogens |
title_full_unstemmed |
In vitro culture of mollusc hemocytes. Functional study of burst respiratory activity and analysis of interactions with protozoan and procaryotic pathogens |
title_sort |
in vitro culture of mollusc hemocytes. functional study of burst respiratory activity and analysis of interactions with protozoan and procaryotic pathogens |
publisher |
Actes du colloque, 8th International Conference on Invertebrate and Fish tissue culture. Tissue culture association, Comubia : pp 56-64 |
publishDate |
1991 |
url |
https://archimer.ifremer.fr/doc/1991/acte-4115.PDF https://archimer.ifremer.fr/doc/00000/4115/ |
genre |
Crassostrea gigas |
genre_facet |
Crassostrea gigas |
op_relation |
https://archimer.ifremer.fr/doc/1991/acte-4115.PDF https://archimer.ifremer.fr/doc/00000/4115/ |
op_rights |
info:eu-repo/semantics/openAccess restricted use |
_version_ |
1766394724837490688 |