Low incidence of clonality in cold water corals revealed through the novel use of standardized protocol adapted to deep sea sampling
Sampling in the deep sea is a technical challenge, which has hindered the acquisition of robust datasets that are necessary to determine the fine-grained biological patterns and processes that may shape genetic diversity. Estimates of the extent of clonality in deep-sea species, despite the importan...
Published in: | Deep Sea Research Part II: Topical Studies in Oceanography |
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Language: | English |
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Pergamon-elsevier Science Ltd
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Online Access: | https://archimer.ifremer.fr/doc/00300/41117/40291.pdf https://doi.org/10.1016/j.dsr2.2015.11.013 https://archimer.ifremer.fr/doc/00300/41117/ |
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ftarchimer:oai:archimer.ifremer.fr:41117 2023-05-15T17:08:41+02:00 Low incidence of clonality in cold water corals revealed through the novel use of standardized protocol adapted to deep sea sampling Becheler, Ronan Cassone, Anne-laure Noel, Philippe Mouchel, Olivier Morrison, Cheryl L. Arnaud-haond, Sophie 2017-11 application/pdf https://archimer.ifremer.fr/doc/00300/41117/40291.pdf https://doi.org/10.1016/j.dsr2.2015.11.013 https://archimer.ifremer.fr/doc/00300/41117/ eng eng Pergamon-elsevier Science Ltd info:eu-repo/grantAgreement/EC/FP7/213144/EU//CORALFISH https://archimer.ifremer.fr/doc/00300/41117/40291.pdf doi:10.1016/j.dsr2.2015.11.013 https://archimer.ifremer.fr/doc/00300/41117/ 2015 Elsevier Ltd. All rights reserved. info:eu-repo/semantics/openAccess restricted use Deep-sea Research Part Ii-topical Studies In Oceanography (0967-0645) (Pergamon-elsevier Science Ltd), 2017-11 , Vol. 145 , P. 120-130 Cold-water coral Lophelia pertusa Madrepora oculata Clonality Fine-grained spatial genetic structure Standardized sampling text Publication info:eu-repo/semantics/article 2017 ftarchimer https://doi.org/10.1016/j.dsr2.2015.11.013 2021-09-23T20:27:07Z Sampling in the deep sea is a technical challenge, which has hindered the acquisition of robust datasets that are necessary to determine the fine-grained biological patterns and processes that may shape genetic diversity. Estimates of the extent of clonality in deep-sea species, despite the importance of clonality in shaping the local dynamics and evolutionary trajectories, have been largely obscured by such limitations. Cold-water coral reefs along European margins are formed mainly by two reef-building species, Lophelia pertusa and Madrepora oculata. Here we present a fine-grained analysis of the genotypic and genetic composition of reefs occurring in the Bay of Biscay, based on an innovative deep-sea sampling protocol. This strategy was designed to be standardized, random, and allowed the georeferencing of all sampled colonies. Clonal lineages discriminated through their Multi-Locus Genotypes (MLG) at 6 to 7 microsatellite markers could thus be mapped to assess the level of clonality and the spatial spread of clonal lineages. High values of clonal richness were observed for both species across all sites suggesting a limited occurrence of clonality, which likely originated through fragmentation. Additionally, spatial autocorrelation analysis underlined the possible occurrence of fine-grained genetic structure in several populations of both L. pertusa and M. oculata. The two cold-water coral species examined had contrasting patterns of connectivity among canyons, with among-canyon genetic structuring detected in M. oculata, whereas L. pertusa was panmictic at the canyon scale. This study exemplifies that a standardized, random and georeferenced sampling strategy, while challenging, can be applied in the deep sea, and associated benefits outlined here include improved estimates of fine grained patterns of clonality and dispersal that are comparable across sites and among species. Article in Journal/Newspaper Lophelia pertusa Archimer (Archive Institutionnelle de l'Ifremer - Institut français de recherche pour l'exploitation de la mer) Deep Sea Research Part II: Topical Studies in Oceanography 145 120 130 |
institution |
Open Polar |
collection |
Archimer (Archive Institutionnelle de l'Ifremer - Institut français de recherche pour l'exploitation de la mer) |
op_collection_id |
ftarchimer |
language |
English |
topic |
Cold-water coral Lophelia pertusa Madrepora oculata Clonality Fine-grained spatial genetic structure Standardized sampling |
spellingShingle |
Cold-water coral Lophelia pertusa Madrepora oculata Clonality Fine-grained spatial genetic structure Standardized sampling Becheler, Ronan Cassone, Anne-laure Noel, Philippe Mouchel, Olivier Morrison, Cheryl L. Arnaud-haond, Sophie Low incidence of clonality in cold water corals revealed through the novel use of standardized protocol adapted to deep sea sampling |
topic_facet |
Cold-water coral Lophelia pertusa Madrepora oculata Clonality Fine-grained spatial genetic structure Standardized sampling |
description |
Sampling in the deep sea is a technical challenge, which has hindered the acquisition of robust datasets that are necessary to determine the fine-grained biological patterns and processes that may shape genetic diversity. Estimates of the extent of clonality in deep-sea species, despite the importance of clonality in shaping the local dynamics and evolutionary trajectories, have been largely obscured by such limitations. Cold-water coral reefs along European margins are formed mainly by two reef-building species, Lophelia pertusa and Madrepora oculata. Here we present a fine-grained analysis of the genotypic and genetic composition of reefs occurring in the Bay of Biscay, based on an innovative deep-sea sampling protocol. This strategy was designed to be standardized, random, and allowed the georeferencing of all sampled colonies. Clonal lineages discriminated through their Multi-Locus Genotypes (MLG) at 6 to 7 microsatellite markers could thus be mapped to assess the level of clonality and the spatial spread of clonal lineages. High values of clonal richness were observed for both species across all sites suggesting a limited occurrence of clonality, which likely originated through fragmentation. Additionally, spatial autocorrelation analysis underlined the possible occurrence of fine-grained genetic structure in several populations of both L. pertusa and M. oculata. The two cold-water coral species examined had contrasting patterns of connectivity among canyons, with among-canyon genetic structuring detected in M. oculata, whereas L. pertusa was panmictic at the canyon scale. This study exemplifies that a standardized, random and georeferenced sampling strategy, while challenging, can be applied in the deep sea, and associated benefits outlined here include improved estimates of fine grained patterns of clonality and dispersal that are comparable across sites and among species. |
format |
Article in Journal/Newspaper |
author |
Becheler, Ronan Cassone, Anne-laure Noel, Philippe Mouchel, Olivier Morrison, Cheryl L. Arnaud-haond, Sophie |
author_facet |
Becheler, Ronan Cassone, Anne-laure Noel, Philippe Mouchel, Olivier Morrison, Cheryl L. Arnaud-haond, Sophie |
author_sort |
Becheler, Ronan |
title |
Low incidence of clonality in cold water corals revealed through the novel use of standardized protocol adapted to deep sea sampling |
title_short |
Low incidence of clonality in cold water corals revealed through the novel use of standardized protocol adapted to deep sea sampling |
title_full |
Low incidence of clonality in cold water corals revealed through the novel use of standardized protocol adapted to deep sea sampling |
title_fullStr |
Low incidence of clonality in cold water corals revealed through the novel use of standardized protocol adapted to deep sea sampling |
title_full_unstemmed |
Low incidence of clonality in cold water corals revealed through the novel use of standardized protocol adapted to deep sea sampling |
title_sort |
low incidence of clonality in cold water corals revealed through the novel use of standardized protocol adapted to deep sea sampling |
publisher |
Pergamon-elsevier Science Ltd |
publishDate |
2017 |
url |
https://archimer.ifremer.fr/doc/00300/41117/40291.pdf https://doi.org/10.1016/j.dsr2.2015.11.013 https://archimer.ifremer.fr/doc/00300/41117/ |
genre |
Lophelia pertusa |
genre_facet |
Lophelia pertusa |
op_source |
Deep-sea Research Part Ii-topical Studies In Oceanography (0967-0645) (Pergamon-elsevier Science Ltd), 2017-11 , Vol. 145 , P. 120-130 |
op_relation |
info:eu-repo/grantAgreement/EC/FP7/213144/EU//CORALFISH https://archimer.ifremer.fr/doc/00300/41117/40291.pdf doi:10.1016/j.dsr2.2015.11.013 https://archimer.ifremer.fr/doc/00300/41117/ |
op_rights |
2015 Elsevier Ltd. All rights reserved. info:eu-repo/semantics/openAccess restricted use |
op_doi |
https://doi.org/10.1016/j.dsr2.2015.11.013 |
container_title |
Deep Sea Research Part II: Topical Studies in Oceanography |
container_volume |
145 |
container_start_page |
120 |
op_container_end_page |
130 |
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1766064502897377280 |