Comparison of hemocyte parameters in the pericardial cavity and the adductor muscle sinus in the Pacific oyster, Crassostrea gigas using two types of flow cytometers
Parameters of hemocyte populations have been considered as relevant indicators of bivalve health and are currently used in immunotoxicological studies. Hemocytes in hemolymph can be collected by puncturing either the pericardial cavity or the adductor muscle sinus with a syringe. Flow cytometry is a...
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ftarchimer:oai:archimer.ifremer.fr:3898 2023-05-15T15:57:39+02:00 Comparison of hemocyte parameters in the pericardial cavity and the adductor muscle sinus in the Pacific oyster, Crassostrea gigas using two types of flow cytometers Gagnaire, Beatrice Duchemin, M Auffret, Mathieu Thomas-guyon, Helene Renault, Tristan 2008 application/pdf https://archimer.ifremer.fr/doc/2008/publication-3898.pdf https://doi.org/10.1051/alr:2008009 https://archimer.ifremer.fr/doc/00000/3898/ eng eng EDP Sciences https://archimer.ifremer.fr/doc/2008/publication-3898.pdf doi:10.1051/alr:2008009 https://archimer.ifremer.fr/doc/00000/3898/ EDP Sciences, IFREMER, IRD 2008 info:eu-repo/semantics/openAccess restricted use Aquatic Living Resources (0990-7440) (EDP Sciences), 2008 , Vol. 21 , N. 1 , P. 39-43 Crassostrea gigas Phagocytosis Hemocytes Flow cytometry text Publication info:eu-repo/semantics/article 2008 ftarchimer https://doi.org/10.1051/alr:2008009 2021-09-23T20:15:45Z Parameters of hemocyte populations have been considered as relevant indicators of bivalve health and are currently used in immunotoxicological studies. Hemocytes in hemolymph can be collected by puncturing either the pericardial cavity or the adductor muscle sinus with a syringe. Flow cytometry is a methodological approach that is increasingly being used in laboratories for the study of hemocyte parameters in aquatic invertebrates. However, various protocols for hemocyte processing in laboratories equipped with different types of cytometers have been published. In this context, two flow cytometers ( EPICS XL4 (R), Beckman Coulter and FacsCalibur (R), Becton Dickinson) and two sites of hemocyte collection ( pericardial cavity and adductor muscle sinus) were compared for the analysis of hemocyte parameters in the Pacific oyster, Crassostrea gigas. Hemolymph cells were analyzed in terms of their number and organelle contents. Cell mortality, phagocytosis, non specific esterase, extension of the lysosomal compartment and production of reactive oxygen species were quantified. The results showed that the phagocytic index was higher for hemocytes obtained in the muscle sinus hemolymph. The results are discussed with respect to the potential use of flow cytometry as a tool for hemocyte studies in bivalves. Article in Journal/Newspaper Crassostrea gigas Pacific oyster Archimer (Archive Institutionnelle de l'Ifremer - Institut français de recherche pour l'exploitation de la mer) Coulter ENVELOPE(-58.033,-58.033,-83.283,-83.283) Pacific |
institution |
Open Polar |
collection |
Archimer (Archive Institutionnelle de l'Ifremer - Institut français de recherche pour l'exploitation de la mer) |
op_collection_id |
ftarchimer |
language |
English |
topic |
Crassostrea gigas Phagocytosis Hemocytes Flow cytometry |
spellingShingle |
Crassostrea gigas Phagocytosis Hemocytes Flow cytometry Gagnaire, Beatrice Duchemin, M Auffret, Mathieu Thomas-guyon, Helene Renault, Tristan Comparison of hemocyte parameters in the pericardial cavity and the adductor muscle sinus in the Pacific oyster, Crassostrea gigas using two types of flow cytometers |
topic_facet |
Crassostrea gigas Phagocytosis Hemocytes Flow cytometry |
description |
Parameters of hemocyte populations have been considered as relevant indicators of bivalve health and are currently used in immunotoxicological studies. Hemocytes in hemolymph can be collected by puncturing either the pericardial cavity or the adductor muscle sinus with a syringe. Flow cytometry is a methodological approach that is increasingly being used in laboratories for the study of hemocyte parameters in aquatic invertebrates. However, various protocols for hemocyte processing in laboratories equipped with different types of cytometers have been published. In this context, two flow cytometers ( EPICS XL4 (R), Beckman Coulter and FacsCalibur (R), Becton Dickinson) and two sites of hemocyte collection ( pericardial cavity and adductor muscle sinus) were compared for the analysis of hemocyte parameters in the Pacific oyster, Crassostrea gigas. Hemolymph cells were analyzed in terms of their number and organelle contents. Cell mortality, phagocytosis, non specific esterase, extension of the lysosomal compartment and production of reactive oxygen species were quantified. The results showed that the phagocytic index was higher for hemocytes obtained in the muscle sinus hemolymph. The results are discussed with respect to the potential use of flow cytometry as a tool for hemocyte studies in bivalves. |
format |
Article in Journal/Newspaper |
author |
Gagnaire, Beatrice Duchemin, M Auffret, Mathieu Thomas-guyon, Helene Renault, Tristan |
author_facet |
Gagnaire, Beatrice Duchemin, M Auffret, Mathieu Thomas-guyon, Helene Renault, Tristan |
author_sort |
Gagnaire, Beatrice |
title |
Comparison of hemocyte parameters in the pericardial cavity and the adductor muscle sinus in the Pacific oyster, Crassostrea gigas using two types of flow cytometers |
title_short |
Comparison of hemocyte parameters in the pericardial cavity and the adductor muscle sinus in the Pacific oyster, Crassostrea gigas using two types of flow cytometers |
title_full |
Comparison of hemocyte parameters in the pericardial cavity and the adductor muscle sinus in the Pacific oyster, Crassostrea gigas using two types of flow cytometers |
title_fullStr |
Comparison of hemocyte parameters in the pericardial cavity and the adductor muscle sinus in the Pacific oyster, Crassostrea gigas using two types of flow cytometers |
title_full_unstemmed |
Comparison of hemocyte parameters in the pericardial cavity and the adductor muscle sinus in the Pacific oyster, Crassostrea gigas using two types of flow cytometers |
title_sort |
comparison of hemocyte parameters in the pericardial cavity and the adductor muscle sinus in the pacific oyster, crassostrea gigas using two types of flow cytometers |
publisher |
EDP Sciences |
publishDate |
2008 |
url |
https://archimer.ifremer.fr/doc/2008/publication-3898.pdf https://doi.org/10.1051/alr:2008009 https://archimer.ifremer.fr/doc/00000/3898/ |
long_lat |
ENVELOPE(-58.033,-58.033,-83.283,-83.283) |
geographic |
Coulter Pacific |
geographic_facet |
Coulter Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
Aquatic Living Resources (0990-7440) (EDP Sciences), 2008 , Vol. 21 , N. 1 , P. 39-43 |
op_relation |
https://archimer.ifremer.fr/doc/2008/publication-3898.pdf doi:10.1051/alr:2008009 https://archimer.ifremer.fr/doc/00000/3898/ |
op_rights |
EDP Sciences, IFREMER, IRD 2008 info:eu-repo/semantics/openAccess restricted use |
op_doi |
https://doi.org/10.1051/alr:2008009 |
_version_ |
1766393327811297280 |