Detoxification of Pacific oyster Crassostrea gigas fed on diets of Skeletonema costatum with and without silt, following PSP contamination by Alexandrium minutum
Contamination of shellfish by paralytic shellfish poisoning ( PSP) toxins poses an economic threat to shellfish farmers. As contaminated shellfish cannot be harvested for long periods of time, it would be very useful to develop processes to optimise and shorten their detoxification. In this study, P...
Main Authors: | , , , , , , |
---|---|
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
EDP Sciences
2008
|
Subjects: | |
Online Access: | https://archimer.ifremer.fr/doc/2008/publication-3896.pdf https://doi.org/10.1051/alr:2008010 https://archimer.ifremer.fr/doc/00000/3896/ |
id |
ftarchimer:oai:archimer.ifremer.fr:3896 |
---|---|
record_format |
openpolar |
spelling |
ftarchimer:oai:archimer.ifremer.fr:3896 2023-05-15T15:57:50+02:00 Detoxification of Pacific oyster Crassostrea gigas fed on diets of Skeletonema costatum with and without silt, following PSP contamination by Alexandrium minutum Gueguen, Marielle Bardouil, Michele Baron, Regis Lassus, Patrick Truquet, Philippe Massardier, Julie Amzil, Zouher 2008 application/pdf https://archimer.ifremer.fr/doc/2008/publication-3896.pdf https://doi.org/10.1051/alr:2008010 https://archimer.ifremer.fr/doc/00000/3896/ eng eng EDP Sciences https://archimer.ifremer.fr/doc/2008/publication-3896.pdf doi:10.1051/alr:2008010 https://archimer.ifremer.fr/doc/00000/3896/ EDP Sciences, IFREMER, IRD 2008 info:eu-repo/semantics/openAccess restricted use Aquatic Living Resources (0990-7440) (EDP Sciences), 2008 , Vol. 21 , N. 1 , P. 13-20 Alexandrium minutum Crassostrea gigas Detoxification Inorganic matter Organic matter PSP Paralytic shellfish poisoning text Publication info:eu-repo/semantics/article 2008 ftarchimer https://doi.org/10.1051/alr:2008010 2021-09-23T20:15:45Z Contamination of shellfish by paralytic shellfish poisoning ( PSP) toxins poses an economic threat to shellfish farmers. As contaminated shellfish cannot be harvested for long periods of time, it would be very useful to develop processes to optimise and shorten their detoxification. In this study, Pacific oysters Crassostrea gigas were first experimentally contaminated over a period of 13 days with a continuous flow of toxic Alexandrium minutum cultures at concentrations ranging from 150 to 200 cell ml(-1) ( toxin content after 13 days of contamination 438 mu g STX equiv. 100 g(-1) wet weight). Then, two different detoxification treatments were tested and showed detoxification rates greater than those observed in coastal environments. The first treatment consisted of feeding oysters on Skeletonema costatum, at a concentration of 2000 cell ml(-1) to speed up detoxification rates. The second detoxification method used the same Skeletonema costatum diet, supplemented with silt particles at a concentration of 20 mg L-1. A control was also set up by placing contaminated oysters in seawater with no additional algal food. The detoxification experiment lasted 8 days. Toxin contents were analysed by liquid chromatography with fluorescence detection ( LC- FD). The S. costatum diet significantly reduced the time needed for oysters to reach the sanitary threshold ( 80 mu g STX equiv. 100 g(-1) wet weight), but no effect of the silt supplement could be demonstrated conclusively. These different detoxification methods did not influence toxin biotransformations as observed in oyster tissues, i. e. epimerisation and decarbamoylation of gonyautoxins 2 and 3. Article in Journal/Newspaper Crassostrea gigas Pacific oyster Archimer (Archive Institutionnelle de l'Ifremer - Institut français de recherche pour l'exploitation de la mer) Pacific |
institution |
Open Polar |
collection |
Archimer (Archive Institutionnelle de l'Ifremer - Institut français de recherche pour l'exploitation de la mer) |
op_collection_id |
ftarchimer |
language |
English |
topic |
Alexandrium minutum Crassostrea gigas Detoxification Inorganic matter Organic matter PSP Paralytic shellfish poisoning |
spellingShingle |
Alexandrium minutum Crassostrea gigas Detoxification Inorganic matter Organic matter PSP Paralytic shellfish poisoning Gueguen, Marielle Bardouil, Michele Baron, Regis Lassus, Patrick Truquet, Philippe Massardier, Julie Amzil, Zouher Detoxification of Pacific oyster Crassostrea gigas fed on diets of Skeletonema costatum with and without silt, following PSP contamination by Alexandrium minutum |
topic_facet |
Alexandrium minutum Crassostrea gigas Detoxification Inorganic matter Organic matter PSP Paralytic shellfish poisoning |
description |
Contamination of shellfish by paralytic shellfish poisoning ( PSP) toxins poses an economic threat to shellfish farmers. As contaminated shellfish cannot be harvested for long periods of time, it would be very useful to develop processes to optimise and shorten their detoxification. In this study, Pacific oysters Crassostrea gigas were first experimentally contaminated over a period of 13 days with a continuous flow of toxic Alexandrium minutum cultures at concentrations ranging from 150 to 200 cell ml(-1) ( toxin content after 13 days of contamination 438 mu g STX equiv. 100 g(-1) wet weight). Then, two different detoxification treatments were tested and showed detoxification rates greater than those observed in coastal environments. The first treatment consisted of feeding oysters on Skeletonema costatum, at a concentration of 2000 cell ml(-1) to speed up detoxification rates. The second detoxification method used the same Skeletonema costatum diet, supplemented with silt particles at a concentration of 20 mg L-1. A control was also set up by placing contaminated oysters in seawater with no additional algal food. The detoxification experiment lasted 8 days. Toxin contents were analysed by liquid chromatography with fluorescence detection ( LC- FD). The S. costatum diet significantly reduced the time needed for oysters to reach the sanitary threshold ( 80 mu g STX equiv. 100 g(-1) wet weight), but no effect of the silt supplement could be demonstrated conclusively. These different detoxification methods did not influence toxin biotransformations as observed in oyster tissues, i. e. epimerisation and decarbamoylation of gonyautoxins 2 and 3. |
format |
Article in Journal/Newspaper |
author |
Gueguen, Marielle Bardouil, Michele Baron, Regis Lassus, Patrick Truquet, Philippe Massardier, Julie Amzil, Zouher |
author_facet |
Gueguen, Marielle Bardouil, Michele Baron, Regis Lassus, Patrick Truquet, Philippe Massardier, Julie Amzil, Zouher |
author_sort |
Gueguen, Marielle |
title |
Detoxification of Pacific oyster Crassostrea gigas fed on diets of Skeletonema costatum with and without silt, following PSP contamination by Alexandrium minutum |
title_short |
Detoxification of Pacific oyster Crassostrea gigas fed on diets of Skeletonema costatum with and without silt, following PSP contamination by Alexandrium minutum |
title_full |
Detoxification of Pacific oyster Crassostrea gigas fed on diets of Skeletonema costatum with and without silt, following PSP contamination by Alexandrium minutum |
title_fullStr |
Detoxification of Pacific oyster Crassostrea gigas fed on diets of Skeletonema costatum with and without silt, following PSP contamination by Alexandrium minutum |
title_full_unstemmed |
Detoxification of Pacific oyster Crassostrea gigas fed on diets of Skeletonema costatum with and without silt, following PSP contamination by Alexandrium minutum |
title_sort |
detoxification of pacific oyster crassostrea gigas fed on diets of skeletonema costatum with and without silt, following psp contamination by alexandrium minutum |
publisher |
EDP Sciences |
publishDate |
2008 |
url |
https://archimer.ifremer.fr/doc/2008/publication-3896.pdf https://doi.org/10.1051/alr:2008010 https://archimer.ifremer.fr/doc/00000/3896/ |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
Aquatic Living Resources (0990-7440) (EDP Sciences), 2008 , Vol. 21 , N. 1 , P. 13-20 |
op_relation |
https://archimer.ifremer.fr/doc/2008/publication-3896.pdf doi:10.1051/alr:2008010 https://archimer.ifremer.fr/doc/00000/3896/ |
op_rights |
EDP Sciences, IFREMER, IRD 2008 info:eu-repo/semantics/openAccess restricted use |
op_doi |
https://doi.org/10.1051/alr:2008010 |
_version_ |
1766393532957851648 |