Assessment of oocyte and trochophore quality in Pacific oyster, Crassostrea gigas
Pacific oyster hatchery production remains highly variable, partially due to inconsistent gamete quality. However, little attention has been paid to oocyte quality in Pacific oyster compared to important vertebrate aquaculture species such as fishes. The aims of the present study were to identify cr...
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ftarchimer:oai:archimer.ifremer.fr:35010 2023-05-15T15:58:07+02:00 Assessment of oocyte and trochophore quality in Pacific oyster, Crassostrea gigas Boulais, Myrina Corporeau, Charlotte Huvet, Arnaud Bernard, Ismael Quere, Claudie Quillien, Virgile Fabioux, Caroline Suquet, Marc 2015-02-01 application/pdf https://archimer.ifremer.fr/doc/00239/35010/33544.pdf https://doi.org/10.1016/j.aquaculture.2014.11.025 https://archimer.ifremer.fr/doc/00239/35010/ eng eng Elsevier Science Bv info:eu-repo/grantAgreement/EC/FP7/245119/EU//REPROSEED https://archimer.ifremer.fr/doc/00239/35010/33544.pdf doi:10.1016/j.aquaculture.2014.11.025 https://archimer.ifremer.fr/doc/00239/35010/ 2014 Published by Elsevier B.V. info:eu-repo/semantics/openAccess restricted use Aquaculture (0044-8486) (Elsevier Science Bv), 2015-02-01 , Vol. 437 , P. 201-207 Crassostrea gigas Oocyte quality Trochophore Lipid Gene expression ATP text Publication info:eu-repo/semantics/article 2015 ftarchimer https://doi.org/10.1016/j.aquaculture.2014.11.025 2021-09-23T20:25:32Z Pacific oyster hatchery production remains highly variable, partially due to inconsistent gamete quality. However, little attention has been paid to oocyte quality in Pacific oyster compared to important vertebrate aquaculture species such as fishes. The aims of the present study were to identify criteria of oocyte and trochophore quality used as predictive tools for embryo developmental success at the D-larval stage. Twenty-six potential proxies of oocyte and trochophore quality were studied on 25 mature females, as determined using histology. Some morphological and biochemical characteristics of mature oocytes were described. Protein appeared to be the main constituent of fully mature Pacific oyster oocytes. Neutral lipids accounted for 61% of the total lipids and triglycerides for 85% of the neutral lipids. Oocyte fertilization capacity was assessed by determining the D-larval yield. High inter-individual variation of D-larval yield was observed (ranging from 1 to 58%). D-larval yield was positively correlated with the percentage of motile trochophores (R2 = 0.237) and with trochophore Velocity of the Average Path (VAP; R2 = 0.255) and negatively correlated with oocyte total steryl esters (R2 = 0.244). The use of a multivariate model, including D-larval yield, trochophore VAP and total steryl esters, led to a higher coefficient of determination (R2 = 0.352), highlighting that the prediction of embryo development in molluscs is a complex process requiring sophisticated multi-factor models. Article in Journal/Newspaper Crassostrea gigas Pacific oyster Archimer (Archive Institutionnelle de l'Ifremer - Institut français de recherche pour l'exploitation de la mer) Pacific Aquaculture 437 201 207 |
institution |
Open Polar |
collection |
Archimer (Archive Institutionnelle de l'Ifremer - Institut français de recherche pour l'exploitation de la mer) |
op_collection_id |
ftarchimer |
language |
English |
topic |
Crassostrea gigas Oocyte quality Trochophore Lipid Gene expression ATP |
spellingShingle |
Crassostrea gigas Oocyte quality Trochophore Lipid Gene expression ATP Boulais, Myrina Corporeau, Charlotte Huvet, Arnaud Bernard, Ismael Quere, Claudie Quillien, Virgile Fabioux, Caroline Suquet, Marc Assessment of oocyte and trochophore quality in Pacific oyster, Crassostrea gigas |
topic_facet |
Crassostrea gigas Oocyte quality Trochophore Lipid Gene expression ATP |
description |
Pacific oyster hatchery production remains highly variable, partially due to inconsistent gamete quality. However, little attention has been paid to oocyte quality in Pacific oyster compared to important vertebrate aquaculture species such as fishes. The aims of the present study were to identify criteria of oocyte and trochophore quality used as predictive tools for embryo developmental success at the D-larval stage. Twenty-six potential proxies of oocyte and trochophore quality were studied on 25 mature females, as determined using histology. Some morphological and biochemical characteristics of mature oocytes were described. Protein appeared to be the main constituent of fully mature Pacific oyster oocytes. Neutral lipids accounted for 61% of the total lipids and triglycerides for 85% of the neutral lipids. Oocyte fertilization capacity was assessed by determining the D-larval yield. High inter-individual variation of D-larval yield was observed (ranging from 1 to 58%). D-larval yield was positively correlated with the percentage of motile trochophores (R2 = 0.237) and with trochophore Velocity of the Average Path (VAP; R2 = 0.255) and negatively correlated with oocyte total steryl esters (R2 = 0.244). The use of a multivariate model, including D-larval yield, trochophore VAP and total steryl esters, led to a higher coefficient of determination (R2 = 0.352), highlighting that the prediction of embryo development in molluscs is a complex process requiring sophisticated multi-factor models. |
format |
Article in Journal/Newspaper |
author |
Boulais, Myrina Corporeau, Charlotte Huvet, Arnaud Bernard, Ismael Quere, Claudie Quillien, Virgile Fabioux, Caroline Suquet, Marc |
author_facet |
Boulais, Myrina Corporeau, Charlotte Huvet, Arnaud Bernard, Ismael Quere, Claudie Quillien, Virgile Fabioux, Caroline Suquet, Marc |
author_sort |
Boulais, Myrina |
title |
Assessment of oocyte and trochophore quality in Pacific oyster, Crassostrea gigas |
title_short |
Assessment of oocyte and trochophore quality in Pacific oyster, Crassostrea gigas |
title_full |
Assessment of oocyte and trochophore quality in Pacific oyster, Crassostrea gigas |
title_fullStr |
Assessment of oocyte and trochophore quality in Pacific oyster, Crassostrea gigas |
title_full_unstemmed |
Assessment of oocyte and trochophore quality in Pacific oyster, Crassostrea gigas |
title_sort |
assessment of oocyte and trochophore quality in pacific oyster, crassostrea gigas |
publisher |
Elsevier Science Bv |
publishDate |
2015 |
url |
https://archimer.ifremer.fr/doc/00239/35010/33544.pdf https://doi.org/10.1016/j.aquaculture.2014.11.025 https://archimer.ifremer.fr/doc/00239/35010/ |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
Aquaculture (0044-8486) (Elsevier Science Bv), 2015-02-01 , Vol. 437 , P. 201-207 |
op_relation |
info:eu-repo/grantAgreement/EC/FP7/245119/EU//REPROSEED https://archimer.ifremer.fr/doc/00239/35010/33544.pdf doi:10.1016/j.aquaculture.2014.11.025 https://archimer.ifremer.fr/doc/00239/35010/ |
op_rights |
2014 Published by Elsevier B.V. info:eu-repo/semantics/openAccess restricted use |
op_doi |
https://doi.org/10.1016/j.aquaculture.2014.11.025 |
container_title |
Aquaculture |
container_volume |
437 |
container_start_page |
201 |
op_container_end_page |
207 |
_version_ |
1766393838429011968 |