Parental exposure to environmental concentrations of diuron leads to aneuploidy in embryos of the Pacific oyster, as evidenced by fluorescent in situ hybridization
Changes in normal chromosome numbers (i.e. aneuploidy) due to abnormal chromosome segregation may arise either spontaneously or as a result of chemical/radiation exposure, particularly during cell division. Coastal ecosystems are continuously subjected to various contaminants originating from urban,...
Published in: | Aquatic Toxicology |
---|---|
Main Authors: | , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Elsevier
2015
|
Subjects: | |
Online Access: | https://archimer.ifremer.fr/doc/00238/34911/33216.pdf https://doi.org/10.1016/j.aquatox.2014.11.011 https://archimer.ifremer.fr/doc/00238/34911/ |
id |
ftarchimer:oai:archimer.ifremer.fr:34911 |
---|---|
record_format |
openpolar |
spelling |
ftarchimer:oai:archimer.ifremer.fr:34911 2023-05-15T15:58:43+02:00 Parental exposure to environmental concentrations of diuron leads to aneuploidy in embryos of the Pacific oyster, as evidenced by fluorescent in situ hybridization Barranger, Audrey Benabdelmouna, Abdellah Degremont, Lionel Burgeot, Thierry Akcha, Farida 2015-02 application/pdf https://archimer.ifremer.fr/doc/00238/34911/33216.pdf https://doi.org/10.1016/j.aquatox.2014.11.011 https://archimer.ifremer.fr/doc/00238/34911/ eng eng Elsevier https://archimer.ifremer.fr/doc/00238/34911/33216.pdf doi:10.1016/j.aquatox.2014.11.011 https://archimer.ifremer.fr/doc/00238/34911/ 2014 Elsevier B.V. All rights reserved. info:eu-repo/semantics/openAccess restricted use Aquatic Toxicology (0166-445X) (Elsevier), 2015-02 , Vol. 159 , P. 36-43 Aneuploidy FISH Diuron Crassostrea gigas text Publication info:eu-repo/semantics/article 2015 ftarchimer https://doi.org/10.1016/j.aquatox.2014.11.011 2021-09-23T20:25:26Z Changes in normal chromosome numbers (i.e. aneuploidy) due to abnormal chromosome segregation may arise either spontaneously or as a result of chemical/radiation exposure, particularly during cell division. Coastal ecosystems are continuously subjected to various contaminants originating from urban, industrial and agricultural activities. Genotoxicity is common to several families of major environmental pollutants, including pesticides, which therefore represent a potential important environmental hazard for marine organisms. A previous study demonstrated the vertical transmission of DNA damage by subjecting oyster genitors to short-term exposure to the herbicide diuron at environmental concentrations during gametogenesis. In this paper, Fluorescent in situ hybridization (FISH) was used to further characterize diuron-induced DNA damage at the chromosomal level. rDNA genes (5S and 18-5.8-28S), previously mapped onto C. gigas chromosomes 4, 5 and 10, were used as probes on the interphase nuclei of embryo preparations. Our results conclusively show higher aneuploidy (hypo- or hyperdiploidy) level in embryos from diuron-exposed genitors, with damage to the three studied chromosomal regions. This study suggests that sexually-developing oysters are vulnerable to diuron exposure, incurring a negative impact on reproductive success and oyster recruitment. Article in Journal/Newspaper Crassostrea gigas Pacific oyster Archimer (Archive Institutionnelle de l'Ifremer - Institut français de recherche pour l'exploitation de la mer) Pacific Aquatic Toxicology 159 36 43 |
institution |
Open Polar |
collection |
Archimer (Archive Institutionnelle de l'Ifremer - Institut français de recherche pour l'exploitation de la mer) |
op_collection_id |
ftarchimer |
language |
English |
topic |
Aneuploidy FISH Diuron Crassostrea gigas |
spellingShingle |
Aneuploidy FISH Diuron Crassostrea gigas Barranger, Audrey Benabdelmouna, Abdellah Degremont, Lionel Burgeot, Thierry Akcha, Farida Parental exposure to environmental concentrations of diuron leads to aneuploidy in embryos of the Pacific oyster, as evidenced by fluorescent in situ hybridization |
topic_facet |
Aneuploidy FISH Diuron Crassostrea gigas |
description |
Changes in normal chromosome numbers (i.e. aneuploidy) due to abnormal chromosome segregation may arise either spontaneously or as a result of chemical/radiation exposure, particularly during cell division. Coastal ecosystems are continuously subjected to various contaminants originating from urban, industrial and agricultural activities. Genotoxicity is common to several families of major environmental pollutants, including pesticides, which therefore represent a potential important environmental hazard for marine organisms. A previous study demonstrated the vertical transmission of DNA damage by subjecting oyster genitors to short-term exposure to the herbicide diuron at environmental concentrations during gametogenesis. In this paper, Fluorescent in situ hybridization (FISH) was used to further characterize diuron-induced DNA damage at the chromosomal level. rDNA genes (5S and 18-5.8-28S), previously mapped onto C. gigas chromosomes 4, 5 and 10, were used as probes on the interphase nuclei of embryo preparations. Our results conclusively show higher aneuploidy (hypo- or hyperdiploidy) level in embryos from diuron-exposed genitors, with damage to the three studied chromosomal regions. This study suggests that sexually-developing oysters are vulnerable to diuron exposure, incurring a negative impact on reproductive success and oyster recruitment. |
format |
Article in Journal/Newspaper |
author |
Barranger, Audrey Benabdelmouna, Abdellah Degremont, Lionel Burgeot, Thierry Akcha, Farida |
author_facet |
Barranger, Audrey Benabdelmouna, Abdellah Degremont, Lionel Burgeot, Thierry Akcha, Farida |
author_sort |
Barranger, Audrey |
title |
Parental exposure to environmental concentrations of diuron leads to aneuploidy in embryos of the Pacific oyster, as evidenced by fluorescent in situ hybridization |
title_short |
Parental exposure to environmental concentrations of diuron leads to aneuploidy in embryos of the Pacific oyster, as evidenced by fluorescent in situ hybridization |
title_full |
Parental exposure to environmental concentrations of diuron leads to aneuploidy in embryos of the Pacific oyster, as evidenced by fluorescent in situ hybridization |
title_fullStr |
Parental exposure to environmental concentrations of diuron leads to aneuploidy in embryos of the Pacific oyster, as evidenced by fluorescent in situ hybridization |
title_full_unstemmed |
Parental exposure to environmental concentrations of diuron leads to aneuploidy in embryos of the Pacific oyster, as evidenced by fluorescent in situ hybridization |
title_sort |
parental exposure to environmental concentrations of diuron leads to aneuploidy in embryos of the pacific oyster, as evidenced by fluorescent in situ hybridization |
publisher |
Elsevier |
publishDate |
2015 |
url |
https://archimer.ifremer.fr/doc/00238/34911/33216.pdf https://doi.org/10.1016/j.aquatox.2014.11.011 https://archimer.ifremer.fr/doc/00238/34911/ |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
Aquatic Toxicology (0166-445X) (Elsevier), 2015-02 , Vol. 159 , P. 36-43 |
op_relation |
https://archimer.ifremer.fr/doc/00238/34911/33216.pdf doi:10.1016/j.aquatox.2014.11.011 https://archimer.ifremer.fr/doc/00238/34911/ |
op_rights |
2014 Elsevier B.V. All rights reserved. info:eu-repo/semantics/openAccess restricted use |
op_doi |
https://doi.org/10.1016/j.aquatox.2014.11.011 |
container_title |
Aquatic Toxicology |
container_volume |
159 |
container_start_page |
36 |
op_container_end_page |
43 |
_version_ |
1766394488318590976 |