Microsatellite-based parentage analysis of factorial crosses in Pacific oyster (Crassostrea gigas) larvae and spat
Several studies have suggested that significant genetic variability might exist for some early developmental traits in the Pacific oyster, C. gigas. However, most studies have examined only limited numbers of families. More critically, these families were rarely replicated, leading to possible bias...
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97. Annu. Meeting of the National Shellfisheries Association
2005
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ftarchimer:oai:archimer.ifremer.fr:3440 2023-05-15T15:57:53+02:00 Microsatellite-based parentage analysis of factorial crosses in Pacific oyster (Crassostrea gigas) larvae and spat Taris, Nicolas Sauvage, Christopher Ernande, Bruno Boudry, Pierre 2005-04-10 application/pdf https://archimer.ifremer.fr/doc/2005/acte-3440.pdf https://archimer.ifremer.fr/doc/00000/3440/ eng eng 97. Annu. Meeting of the National Shellfisheries Association https://archimer.ifremer.fr/doc/2005/acte-3440.pdf https://archimer.ifremer.fr/doc/00000/3440/ info:eu-repo/semantics/openAccess restricted use Selection Genetic variability Microsatellites Crassostrea gigas Pacific oysters text Slideshow info:eu-repo/semantics/conferenceObject 2005 ftarchimer 2021-09-23T20:15:24Z Several studies have suggested that significant genetic variability might exist for some early developmental traits in the Pacific oyster, C. gigas. However, most studies have examined only limited numbers of families. More critically, these families were rarely replicated, leading to possible bias due to heterogeneous larval rearing conditions among families. To overcome these potential problems, we used a mixed-family approach with subsequent parentage analysis. A recently developed set of three multiplexed microsatellite markers was used to determine parentage in a complete factorial cross between 12 males and 4 females. Larvae were reared at two different temperatures (20 and 26°C) to provide a contrast resembling wild versus hatchery conditions. Samples were collected at the larval stage just before settlement and 2 months later. We observed significant male and female effects on larval growth and survival. More importantly, significant genotype x environment interactions were also observed for these traits. The potential selective pressures related to intensive rearing practices in commercial hatcheries will be discussed in the light of our findings. Conference Object Crassostrea gigas Pacific oyster Archimer (Archive Institutionnelle de l'Ifremer - Institut français de recherche pour l'exploitation de la mer) Pacific |
institution |
Open Polar |
collection |
Archimer (Archive Institutionnelle de l'Ifremer - Institut français de recherche pour l'exploitation de la mer) |
op_collection_id |
ftarchimer |
language |
English |
topic |
Selection Genetic variability Microsatellites Crassostrea gigas Pacific oysters |
spellingShingle |
Selection Genetic variability Microsatellites Crassostrea gigas Pacific oysters Taris, Nicolas Sauvage, Christopher Ernande, Bruno Boudry, Pierre Microsatellite-based parentage analysis of factorial crosses in Pacific oyster (Crassostrea gigas) larvae and spat |
topic_facet |
Selection Genetic variability Microsatellites Crassostrea gigas Pacific oysters |
description |
Several studies have suggested that significant genetic variability might exist for some early developmental traits in the Pacific oyster, C. gigas. However, most studies have examined only limited numbers of families. More critically, these families were rarely replicated, leading to possible bias due to heterogeneous larval rearing conditions among families. To overcome these potential problems, we used a mixed-family approach with subsequent parentage analysis. A recently developed set of three multiplexed microsatellite markers was used to determine parentage in a complete factorial cross between 12 males and 4 females. Larvae were reared at two different temperatures (20 and 26°C) to provide a contrast resembling wild versus hatchery conditions. Samples were collected at the larval stage just before settlement and 2 months later. We observed significant male and female effects on larval growth and survival. More importantly, significant genotype x environment interactions were also observed for these traits. The potential selective pressures related to intensive rearing practices in commercial hatcheries will be discussed in the light of our findings. |
format |
Conference Object |
author |
Taris, Nicolas Sauvage, Christopher Ernande, Bruno Boudry, Pierre |
author_facet |
Taris, Nicolas Sauvage, Christopher Ernande, Bruno Boudry, Pierre |
author_sort |
Taris, Nicolas |
title |
Microsatellite-based parentage analysis of factorial crosses in Pacific oyster (Crassostrea gigas) larvae and spat |
title_short |
Microsatellite-based parentage analysis of factorial crosses in Pacific oyster (Crassostrea gigas) larvae and spat |
title_full |
Microsatellite-based parentage analysis of factorial crosses in Pacific oyster (Crassostrea gigas) larvae and spat |
title_fullStr |
Microsatellite-based parentage analysis of factorial crosses in Pacific oyster (Crassostrea gigas) larvae and spat |
title_full_unstemmed |
Microsatellite-based parentage analysis of factorial crosses in Pacific oyster (Crassostrea gigas) larvae and spat |
title_sort |
microsatellite-based parentage analysis of factorial crosses in pacific oyster (crassostrea gigas) larvae and spat |
publisher |
97. Annu. Meeting of the National Shellfisheries Association |
publishDate |
2005 |
url |
https://archimer.ifremer.fr/doc/2005/acte-3440.pdf https://archimer.ifremer.fr/doc/00000/3440/ |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_relation |
https://archimer.ifremer.fr/doc/2005/acte-3440.pdf https://archimer.ifremer.fr/doc/00000/3440/ |
op_rights |
info:eu-repo/semantics/openAccess restricted use |
_version_ |
1766393595371192320 |