A Feedback Mechanism to Control Apoptosis Occurs in the Digestive Gland of the Oyster Crassostrea gigas Exposed to the Paralytic Shellfish Toxins Producer Alexandrium catenella
To better understand the effect of Paralytic Shellfish Toxins (PSTs) accumulation in the digestive gland of the Pacific oyster, Crassostrea gigas, we experimentally exposed individual oysters for 48 h to a PSTs producer, the dinoflagellate Alexandrium catenella. In comparison to the effect of the no...
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ftarchimer:oai:archimer.ifremer.fr:33606 2023-05-15T15:58:07+02:00 A Feedback Mechanism to Control Apoptosis Occurs in the Digestive Gland of the Oyster Crassostrea gigas Exposed to the Paralytic Shellfish Toxins Producer Alexandrium catenella Rolland, Jean-luc Medhioub, Walid Vergnes, Agnes Abi-khalil, Celina Savar, Veronique Abadie, Eric Masseret, Estelle Amzil, Zouher Laabir, Mohamed 2014-09 application/pdf https://archimer.ifremer.fr/doc/00225/33606/32003.pdf https://doi.org/10.3390/md12095035 https://archimer.ifremer.fr/doc/00225/33606/ eng eng Mdpi Ag https://archimer.ifremer.fr/doc/00225/33606/32003.pdf doi:10.3390/md12095035 https://archimer.ifremer.fr/doc/00225/33606/ 2014 MDPI AG (Basel, Switzerland) unless otherwise stated info:eu-repo/semantics/openAccess restricted use Marine Drugs (1660-3397) (Mdpi Ag), 2014-09 , Vol. 12 , N. 9 , P. 5035-5054 shellfish toxin biomarker expression phytoplankton text Publication info:eu-repo/semantics/article 2014 ftarchimer https://doi.org/10.3390/md12095035 2021-09-23T20:25:10Z To better understand the effect of Paralytic Shellfish Toxins (PSTs) accumulation in the digestive gland of the Pacific oyster, Crassostrea gigas, we experimentally exposed individual oysters for 48 h to a PSTs producer, the dinoflagellate Alexandrium catenella. In comparison to the effect of the non-toxic Alexandrium tamarense, on the eight apoptotic related genes tested, Bax and BI.1 were significantly upregulated in oysters exposed 48 h to A. catenella. Among the five detoxification related genes tested, the expression of cytochrome P450 (CYP1A) was shown to be correlated with toxin concentration in the digestive gland of oysters exposed to the toxic dinoflagellate. Beside this, we observed a significant increase in ROS production, a decrease in caspase-3/7 activity and normal percentage of apoptotic cells in this tissue. Taken together, these results suggest a feedback mechanism, which may occur in the digestive gland where BI.1 could play a key role in preventing the induction of apoptosis by PSTs. Moreover, the expression of CYP1A, Bax and BI.1 were found to be significantly correlated to the occurrence of natural toxic events, suggesting that the expression of these genes together could be used as biomarker to assess the biological responses of oysters to stress caused by PSTs. Article in Journal/Newspaper Crassostrea gigas Pacific oyster Archimer (Archive Institutionnelle de l'Ifremer - Institut français de recherche pour l'exploitation de la mer) Pacific Marine Drugs 12 9 5035 5054 |
institution |
Open Polar |
collection |
Archimer (Archive Institutionnelle de l'Ifremer - Institut français de recherche pour l'exploitation de la mer) |
op_collection_id |
ftarchimer |
language |
English |
topic |
shellfish toxin biomarker expression phytoplankton |
spellingShingle |
shellfish toxin biomarker expression phytoplankton Rolland, Jean-luc Medhioub, Walid Vergnes, Agnes Abi-khalil, Celina Savar, Veronique Abadie, Eric Masseret, Estelle Amzil, Zouher Laabir, Mohamed A Feedback Mechanism to Control Apoptosis Occurs in the Digestive Gland of the Oyster Crassostrea gigas Exposed to the Paralytic Shellfish Toxins Producer Alexandrium catenella |
topic_facet |
shellfish toxin biomarker expression phytoplankton |
description |
To better understand the effect of Paralytic Shellfish Toxins (PSTs) accumulation in the digestive gland of the Pacific oyster, Crassostrea gigas, we experimentally exposed individual oysters for 48 h to a PSTs producer, the dinoflagellate Alexandrium catenella. In comparison to the effect of the non-toxic Alexandrium tamarense, on the eight apoptotic related genes tested, Bax and BI.1 were significantly upregulated in oysters exposed 48 h to A. catenella. Among the five detoxification related genes tested, the expression of cytochrome P450 (CYP1A) was shown to be correlated with toxin concentration in the digestive gland of oysters exposed to the toxic dinoflagellate. Beside this, we observed a significant increase in ROS production, a decrease in caspase-3/7 activity and normal percentage of apoptotic cells in this tissue. Taken together, these results suggest a feedback mechanism, which may occur in the digestive gland where BI.1 could play a key role in preventing the induction of apoptosis by PSTs. Moreover, the expression of CYP1A, Bax and BI.1 were found to be significantly correlated to the occurrence of natural toxic events, suggesting that the expression of these genes together could be used as biomarker to assess the biological responses of oysters to stress caused by PSTs. |
format |
Article in Journal/Newspaper |
author |
Rolland, Jean-luc Medhioub, Walid Vergnes, Agnes Abi-khalil, Celina Savar, Veronique Abadie, Eric Masseret, Estelle Amzil, Zouher Laabir, Mohamed |
author_facet |
Rolland, Jean-luc Medhioub, Walid Vergnes, Agnes Abi-khalil, Celina Savar, Veronique Abadie, Eric Masseret, Estelle Amzil, Zouher Laabir, Mohamed |
author_sort |
Rolland, Jean-luc |
title |
A Feedback Mechanism to Control Apoptosis Occurs in the Digestive Gland of the Oyster Crassostrea gigas Exposed to the Paralytic Shellfish Toxins Producer Alexandrium catenella |
title_short |
A Feedback Mechanism to Control Apoptosis Occurs in the Digestive Gland of the Oyster Crassostrea gigas Exposed to the Paralytic Shellfish Toxins Producer Alexandrium catenella |
title_full |
A Feedback Mechanism to Control Apoptosis Occurs in the Digestive Gland of the Oyster Crassostrea gigas Exposed to the Paralytic Shellfish Toxins Producer Alexandrium catenella |
title_fullStr |
A Feedback Mechanism to Control Apoptosis Occurs in the Digestive Gland of the Oyster Crassostrea gigas Exposed to the Paralytic Shellfish Toxins Producer Alexandrium catenella |
title_full_unstemmed |
A Feedback Mechanism to Control Apoptosis Occurs in the Digestive Gland of the Oyster Crassostrea gigas Exposed to the Paralytic Shellfish Toxins Producer Alexandrium catenella |
title_sort |
feedback mechanism to control apoptosis occurs in the digestive gland of the oyster crassostrea gigas exposed to the paralytic shellfish toxins producer alexandrium catenella |
publisher |
Mdpi Ag |
publishDate |
2014 |
url |
https://archimer.ifremer.fr/doc/00225/33606/32003.pdf https://doi.org/10.3390/md12095035 https://archimer.ifremer.fr/doc/00225/33606/ |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
Marine Drugs (1660-3397) (Mdpi Ag), 2014-09 , Vol. 12 , N. 9 , P. 5035-5054 |
op_relation |
https://archimer.ifremer.fr/doc/00225/33606/32003.pdf doi:10.3390/md12095035 https://archimer.ifremer.fr/doc/00225/33606/ |
op_rights |
2014 MDPI AG (Basel, Switzerland) unless otherwise stated info:eu-repo/semantics/openAccess restricted use |
op_doi |
https://doi.org/10.3390/md12095035 |
container_title |
Marine Drugs |
container_volume |
12 |
container_issue |
9 |
container_start_page |
5035 |
op_container_end_page |
5054 |
_version_ |
1766393838252851200 |