Natural hybridization between genetically differentiated populations of Crassostrea gigas and C-angulata highlighted by sequence variation in flanking regions of a microsatellite locus
The marine environment is of special interest for studying hybridization between closely related taxa because of the high dispersal potential of planktonic larvae, such as those of most bivalve species. The oysters Crassostrea angulata and C. gigas are known to be very close genetically and entirely...
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ftarchimer:oai:archimer.ifremer.fr:3355 2023-05-15T15:58:22+02:00 Natural hybridization between genetically differentiated populations of Crassostrea gigas and C-angulata highlighted by sequence variation in flanking regions of a microsatellite locus Huvet, Arnaud Fabioux, Caroline Mccombie, Helen Lapegue, Sylvie Boudry, Pierre 2004 application/pdf https://archimer.ifremer.fr/doc/2004/publication-3355.pdf https://archimer.ifremer.fr/doc/00000/3355/ eng eng Inter-Research https://archimer.ifremer.fr/doc/2004/publication-3355.pdf https://archimer.ifremer.fr/doc/00000/3355/ info:eu-repo/semantics/openAccess restricted use Marine Ecology Progress Series (0171-8630) (Inter-Research), 2004 , Vol. 272 , P. 141-152 Allele size Microsatellites Genetic differentiation Hybridization Crassostrea gigas Crassostrea angulata text Publication info:eu-repo/semantics/article 2004 ftarchimer 2021-09-23T20:15:16Z The marine environment is of special interest for studying hybridization between closely related taxa because of the high dispersal potential of planktonic larvae, such as those of most bivalve species. The oysters Crassostrea angulata and C. gigas are known to be very close genetically and entirely inter-fertile under controlled conditions. However, hybridization in the wild had not been investigated, mainly due to the lack of nuclear diagnostic markers. In the present paper, we first estimated genetic differentiation between these 2 closely related taxa using 8 microsatellite markers. Interestingly, 5 markers displayed significant differences of allele size between taxa. The subsequent sequencing of alleles of one of these microsatellites showed several mutational events, which suggested null alleles and homoplasy. The presence of 1 insertion/deletion event in its 5' flanking sequence enabled us to design a new bi-allelic ('C' and 'NC') nuclear PCR-restriction fragment length polymorphism (-RFLP) marker (CG44R). This, together with a mitochondrial DNA marker, was used to analyze populations of C. angulata and C. gigas. The CG44R allele frequencies were very different between C. angulata (f[C] = 0.91) and C. gigas (f[NC] = 0.92) populations. This analysis also provided evidence for hybridization between C. angulata and C. gigas in a wild Portuguese population where the 2 taxa are in contact due to recent transportation of C. gigas stocks for aquacultural production. Our results represent the first indication of hybridization between these 2 taxa in the natural environment, and contribute to knowledge of the evolutionary history of the Crassostrea genus. Article in Journal/Newspaper Crassostrea gigas Archimer (Archive Institutionnelle de l'Ifremer - Institut français de recherche pour l'exploitation de la mer) |
institution |
Open Polar |
collection |
Archimer (Archive Institutionnelle de l'Ifremer - Institut français de recherche pour l'exploitation de la mer) |
op_collection_id |
ftarchimer |
language |
English |
topic |
Allele size Microsatellites Genetic differentiation Hybridization Crassostrea gigas Crassostrea angulata |
spellingShingle |
Allele size Microsatellites Genetic differentiation Hybridization Crassostrea gigas Crassostrea angulata Huvet, Arnaud Fabioux, Caroline Mccombie, Helen Lapegue, Sylvie Boudry, Pierre Natural hybridization between genetically differentiated populations of Crassostrea gigas and C-angulata highlighted by sequence variation in flanking regions of a microsatellite locus |
topic_facet |
Allele size Microsatellites Genetic differentiation Hybridization Crassostrea gigas Crassostrea angulata |
description |
The marine environment is of special interest for studying hybridization between closely related taxa because of the high dispersal potential of planktonic larvae, such as those of most bivalve species. The oysters Crassostrea angulata and C. gigas are known to be very close genetically and entirely inter-fertile under controlled conditions. However, hybridization in the wild had not been investigated, mainly due to the lack of nuclear diagnostic markers. In the present paper, we first estimated genetic differentiation between these 2 closely related taxa using 8 microsatellite markers. Interestingly, 5 markers displayed significant differences of allele size between taxa. The subsequent sequencing of alleles of one of these microsatellites showed several mutational events, which suggested null alleles and homoplasy. The presence of 1 insertion/deletion event in its 5' flanking sequence enabled us to design a new bi-allelic ('C' and 'NC') nuclear PCR-restriction fragment length polymorphism (-RFLP) marker (CG44R). This, together with a mitochondrial DNA marker, was used to analyze populations of C. angulata and C. gigas. The CG44R allele frequencies were very different between C. angulata (f[C] = 0.91) and C. gigas (f[NC] = 0.92) populations. This analysis also provided evidence for hybridization between C. angulata and C. gigas in a wild Portuguese population where the 2 taxa are in contact due to recent transportation of C. gigas stocks for aquacultural production. Our results represent the first indication of hybridization between these 2 taxa in the natural environment, and contribute to knowledge of the evolutionary history of the Crassostrea genus. |
format |
Article in Journal/Newspaper |
author |
Huvet, Arnaud Fabioux, Caroline Mccombie, Helen Lapegue, Sylvie Boudry, Pierre |
author_facet |
Huvet, Arnaud Fabioux, Caroline Mccombie, Helen Lapegue, Sylvie Boudry, Pierre |
author_sort |
Huvet, Arnaud |
title |
Natural hybridization between genetically differentiated populations of Crassostrea gigas and C-angulata highlighted by sequence variation in flanking regions of a microsatellite locus |
title_short |
Natural hybridization between genetically differentiated populations of Crassostrea gigas and C-angulata highlighted by sequence variation in flanking regions of a microsatellite locus |
title_full |
Natural hybridization between genetically differentiated populations of Crassostrea gigas and C-angulata highlighted by sequence variation in flanking regions of a microsatellite locus |
title_fullStr |
Natural hybridization between genetically differentiated populations of Crassostrea gigas and C-angulata highlighted by sequence variation in flanking regions of a microsatellite locus |
title_full_unstemmed |
Natural hybridization between genetically differentiated populations of Crassostrea gigas and C-angulata highlighted by sequence variation in flanking regions of a microsatellite locus |
title_sort |
natural hybridization between genetically differentiated populations of crassostrea gigas and c-angulata highlighted by sequence variation in flanking regions of a microsatellite locus |
publisher |
Inter-Research |
publishDate |
2004 |
url |
https://archimer.ifremer.fr/doc/2004/publication-3355.pdf https://archimer.ifremer.fr/doc/00000/3355/ |
genre |
Crassostrea gigas |
genre_facet |
Crassostrea gigas |
op_source |
Marine Ecology Progress Series (0171-8630) (Inter-Research), 2004 , Vol. 272 , P. 141-152 |
op_relation |
https://archimer.ifremer.fr/doc/2004/publication-3355.pdf https://archimer.ifremer.fr/doc/00000/3355/ |
op_rights |
info:eu-repo/semantics/openAccess restricted use |
_version_ |
1766394107891023872 |