Development of SNPs and their use for the identification of summer mortality resistance QTLs in the Pacific oyster (Crassotrea gigas)
Summer mortalities of the Pacific cupped oyster Crassostrea gigas have been described in the literature for decades. One solution to overcome this problem would be to select animals more tolerant to summer mortalities, as this trait was shown to be highly heritable (Degrémont, 2003). The identificat...
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International Association for Genetics in Aquaculture (IAGA)
2006
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ftarchimer:oai:archimer.ifremer.fr:3351 2023-05-15T15:58:08+02:00 Development of SNPs and their use for the identification of summer mortality resistance QTLs in the Pacific oyster (Crassotrea gigas) Sauvage, Christopher Lapegue, Sylvie Bierne, Nicolas Boudry, Pierre 2006-06-26 application/pdf https://archimer.ifremer.fr/doc/2006/acte-3351.pdf https://archimer.ifremer.fr/doc/00000/3351/ eng eng International Association for Genetics in Aquaculture (IAGA) https://archimer.ifremer.fr/doc/2006/acte-3351.pdf https://archimer.ifremer.fr/doc/00000/3351/ info:eu-repo/semantics/openAccess restricted use QTLs analyses Summer mortaliy Genetic SNPs Crassostrea gigas Oyster text Poster info:eu-repo/semantics/conferenceObject 2006 ftarchimer 2021-09-23T20:15:16Z Summer mortalities of the Pacific cupped oyster Crassostrea gigas have been described in the literature for decades. One solution to overcome this problem would be to select animals more tolerant to summer mortalities, as this trait was shown to be highly heritable (Degrémont, 2003). The identification of markers associated to the resistance would allow to better understand this complex phenomenon and, eventually to establish markers assisted selection. Recently, two moderately dense genetic linkage maps have been established in the Pacific oyster based on microsatellites and AFLP markers (Hubert and Hedgecock, 2004; Li and Guo, 2004). In this context, the development and mapping of SNPs will increase the density of the linkage map and consequently the accuracy of QTL mapping. To reach this objective, crosses between "resistant" and "sensitive" mapping parents (F0) was performed in 2003 to generate F1 families. Segregating F2 families will be produced in 2006 for SNP mapping and QTL analyses. The development of SNPs is in progress. We identify SNPs by the direct sequencing of ESTs. Our first results show a high level of polymorphism (from 1 to 5 SNPs / 100 bp) and only 3% of the EST are monomorphic. Each SNP will be scored by mass spectrometry to validate each marker and to detect heterozygote loci in F1 and F2 progenies. Conference Object Crassostrea gigas Pacific oyster Archimer (Archive Institutionnelle de l'Ifremer - Institut français de recherche pour l'exploitation de la mer) Pacific |
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Open Polar |
collection |
Archimer (Archive Institutionnelle de l'Ifremer - Institut français de recherche pour l'exploitation de la mer) |
op_collection_id |
ftarchimer |
language |
English |
topic |
QTLs analyses Summer mortaliy Genetic SNPs Crassostrea gigas Oyster |
spellingShingle |
QTLs analyses Summer mortaliy Genetic SNPs Crassostrea gigas Oyster Sauvage, Christopher Lapegue, Sylvie Bierne, Nicolas Boudry, Pierre Development of SNPs and their use for the identification of summer mortality resistance QTLs in the Pacific oyster (Crassotrea gigas) |
topic_facet |
QTLs analyses Summer mortaliy Genetic SNPs Crassostrea gigas Oyster |
description |
Summer mortalities of the Pacific cupped oyster Crassostrea gigas have been described in the literature for decades. One solution to overcome this problem would be to select animals more tolerant to summer mortalities, as this trait was shown to be highly heritable (Degrémont, 2003). The identification of markers associated to the resistance would allow to better understand this complex phenomenon and, eventually to establish markers assisted selection. Recently, two moderately dense genetic linkage maps have been established in the Pacific oyster based on microsatellites and AFLP markers (Hubert and Hedgecock, 2004; Li and Guo, 2004). In this context, the development and mapping of SNPs will increase the density of the linkage map and consequently the accuracy of QTL mapping. To reach this objective, crosses between "resistant" and "sensitive" mapping parents (F0) was performed in 2003 to generate F1 families. Segregating F2 families will be produced in 2006 for SNP mapping and QTL analyses. The development of SNPs is in progress. We identify SNPs by the direct sequencing of ESTs. Our first results show a high level of polymorphism (from 1 to 5 SNPs / 100 bp) and only 3% of the EST are monomorphic. Each SNP will be scored by mass spectrometry to validate each marker and to detect heterozygote loci in F1 and F2 progenies. |
format |
Conference Object |
author |
Sauvage, Christopher Lapegue, Sylvie Bierne, Nicolas Boudry, Pierre |
author_facet |
Sauvage, Christopher Lapegue, Sylvie Bierne, Nicolas Boudry, Pierre |
author_sort |
Sauvage, Christopher |
title |
Development of SNPs and their use for the identification of summer mortality resistance QTLs in the Pacific oyster (Crassotrea gigas) |
title_short |
Development of SNPs and their use for the identification of summer mortality resistance QTLs in the Pacific oyster (Crassotrea gigas) |
title_full |
Development of SNPs and their use for the identification of summer mortality resistance QTLs in the Pacific oyster (Crassotrea gigas) |
title_fullStr |
Development of SNPs and their use for the identification of summer mortality resistance QTLs in the Pacific oyster (Crassotrea gigas) |
title_full_unstemmed |
Development of SNPs and their use for the identification of summer mortality resistance QTLs in the Pacific oyster (Crassotrea gigas) |
title_sort |
development of snps and their use for the identification of summer mortality resistance qtls in the pacific oyster (crassotrea gigas) |
publisher |
International Association for Genetics in Aquaculture (IAGA) |
publishDate |
2006 |
url |
https://archimer.ifremer.fr/doc/2006/acte-3351.pdf https://archimer.ifremer.fr/doc/00000/3351/ |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_relation |
https://archimer.ifremer.fr/doc/2006/acte-3351.pdf https://archimer.ifremer.fr/doc/00000/3351/ |
op_rights |
info:eu-repo/semantics/openAccess restricted use |
_version_ |
1766393862313476096 |