Antimicrobial histones and DNA traps in invertebrate immunity: evidences in Crassostrea gigas.
Although antimicrobial histones have been isolated from multiple metazoan species, their role in host defense has long remained unanswered. We found here that the hemocytes of the oyster Crassostrea gigas release antimicrobial H1-like and H5-like histones in response to tissue damage and infection....
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ftarchimer:oai:archimer.ifremer.fr:32866 2023-05-15T15:58:01+02:00 Antimicrobial histones and DNA traps in invertebrate immunity: evidences in Crassostrea gigas. Poirier, Aurore C. Schmitt, Paulina Rosa, Rafael D. Vanhove, Audrey S. Kieffer-jaquinod, Sylvie Rubio, Tristan P. Charriere, Guillaume M. Destoumieux-garzon, Delphine 2014-09-05 application/pdf https://archimer.ifremer.fr/doc/00217/32866/31784.pdf https://doi.org/10.1074/jbc.M114.576546 https://archimer.ifremer.fr/doc/00217/32866/ eng eng Amer Soc Biochemistry Molecular Biology Inc https://archimer.ifremer.fr/doc/00217/32866/31784.pdf doi:10.1074/jbc.M114.576546 https://archimer.ifremer.fr/doc/00217/32866/ 2014 by The American Society for Biochemistry and Molecular Biology, Inc. info:eu-repo/semantics/openAccess restricted use Journal Of Biological Chemistry (0021-9258) (Amer Soc Biochemistry Molecular Biology Inc), 2014-09-05 , Vol. 289 , N. 36 , P. 24821-24831 Antimicrobial Peptide (AMP) DNA Innate Immunity Invertebrate Reactive Oxygen Species (ROS) NET Mollusk text Publication info:eu-repo/semantics/article 2014 ftarchimer https://doi.org/10.1074/jbc.M114.576546 2021-09-23T20:25:01Z Although antimicrobial histones have been isolated from multiple metazoan species, their role in host defense has long remained unanswered. We found here that the hemocytes of the oyster Crassostrea gigas release antimicrobial H1-like and H5-like histones in response to tissue damage and infection. These antimicrobial histones were shown to be associated with extracellular DNA networks released by hemocytes, the circulating immune cells of invertebrates, in response to immune challenge. The hemocyte-released DNA was found to surround and entangle vibrios. This defense mechanism is reminiscent of the neutrophil extracellular traps (ETs) recently described in vertebrates. Importantly, oyster ETs were evidenced in vivo in hemocyte-infiltrated interstitial tissues surrounding wounds, whereas they were absent from tissues of unchallenged oysters. Consistently, antimicrobial histones were found to accumulate in oyster tissues following injury or infection with vibrios. Finally, oyster ET formation was highly dependent on the production of reactive oxygen species by hemocytes. This shows that ET formation relies on common cellular and molecular mechanisms from vertebrates to invertebrates. Altogether, our data reveal that ET formation is a defense mechanism triggered by infection and tissue damage, which is shared by relatively distant species suggesting either evolutionary conservation or convergent evolution within Bilateria. Article in Journal/Newspaper Crassostrea gigas Archimer (Archive Institutionnelle de l'Ifremer - Institut français de recherche pour l'exploitation de la mer) Journal of Biological Chemistry 289 36 24821 24831 |
institution |
Open Polar |
collection |
Archimer (Archive Institutionnelle de l'Ifremer - Institut français de recherche pour l'exploitation de la mer) |
op_collection_id |
ftarchimer |
language |
English |
topic |
Antimicrobial Peptide (AMP) DNA Innate Immunity Invertebrate Reactive Oxygen Species (ROS) NET Mollusk |
spellingShingle |
Antimicrobial Peptide (AMP) DNA Innate Immunity Invertebrate Reactive Oxygen Species (ROS) NET Mollusk Poirier, Aurore C. Schmitt, Paulina Rosa, Rafael D. Vanhove, Audrey S. Kieffer-jaquinod, Sylvie Rubio, Tristan P. Charriere, Guillaume M. Destoumieux-garzon, Delphine Antimicrobial histones and DNA traps in invertebrate immunity: evidences in Crassostrea gigas. |
topic_facet |
Antimicrobial Peptide (AMP) DNA Innate Immunity Invertebrate Reactive Oxygen Species (ROS) NET Mollusk |
description |
Although antimicrobial histones have been isolated from multiple metazoan species, their role in host defense has long remained unanswered. We found here that the hemocytes of the oyster Crassostrea gigas release antimicrobial H1-like and H5-like histones in response to tissue damage and infection. These antimicrobial histones were shown to be associated with extracellular DNA networks released by hemocytes, the circulating immune cells of invertebrates, in response to immune challenge. The hemocyte-released DNA was found to surround and entangle vibrios. This defense mechanism is reminiscent of the neutrophil extracellular traps (ETs) recently described in vertebrates. Importantly, oyster ETs were evidenced in vivo in hemocyte-infiltrated interstitial tissues surrounding wounds, whereas they were absent from tissues of unchallenged oysters. Consistently, antimicrobial histones were found to accumulate in oyster tissues following injury or infection with vibrios. Finally, oyster ET formation was highly dependent on the production of reactive oxygen species by hemocytes. This shows that ET formation relies on common cellular and molecular mechanisms from vertebrates to invertebrates. Altogether, our data reveal that ET formation is a defense mechanism triggered by infection and tissue damage, which is shared by relatively distant species suggesting either evolutionary conservation or convergent evolution within Bilateria. |
format |
Article in Journal/Newspaper |
author |
Poirier, Aurore C. Schmitt, Paulina Rosa, Rafael D. Vanhove, Audrey S. Kieffer-jaquinod, Sylvie Rubio, Tristan P. Charriere, Guillaume M. Destoumieux-garzon, Delphine |
author_facet |
Poirier, Aurore C. Schmitt, Paulina Rosa, Rafael D. Vanhove, Audrey S. Kieffer-jaquinod, Sylvie Rubio, Tristan P. Charriere, Guillaume M. Destoumieux-garzon, Delphine |
author_sort |
Poirier, Aurore C. |
title |
Antimicrobial histones and DNA traps in invertebrate immunity: evidences in Crassostrea gigas. |
title_short |
Antimicrobial histones and DNA traps in invertebrate immunity: evidences in Crassostrea gigas. |
title_full |
Antimicrobial histones and DNA traps in invertebrate immunity: evidences in Crassostrea gigas. |
title_fullStr |
Antimicrobial histones and DNA traps in invertebrate immunity: evidences in Crassostrea gigas. |
title_full_unstemmed |
Antimicrobial histones and DNA traps in invertebrate immunity: evidences in Crassostrea gigas. |
title_sort |
antimicrobial histones and dna traps in invertebrate immunity: evidences in crassostrea gigas. |
publisher |
Amer Soc Biochemistry Molecular Biology Inc |
publishDate |
2014 |
url |
https://archimer.ifremer.fr/doc/00217/32866/31784.pdf https://doi.org/10.1074/jbc.M114.576546 https://archimer.ifremer.fr/doc/00217/32866/ |
genre |
Crassostrea gigas |
genre_facet |
Crassostrea gigas |
op_source |
Journal Of Biological Chemistry (0021-9258) (Amer Soc Biochemistry Molecular Biology Inc), 2014-09-05 , Vol. 289 , N. 36 , P. 24821-24831 |
op_relation |
https://archimer.ifremer.fr/doc/00217/32866/31784.pdf doi:10.1074/jbc.M114.576546 https://archimer.ifremer.fr/doc/00217/32866/ |
op_rights |
2014 by The American Society for Biochemistry and Molecular Biology, Inc. info:eu-repo/semantics/openAccess restricted use |
op_doi |
https://doi.org/10.1074/jbc.M114.576546 |
container_title |
Journal of Biological Chemistry |
container_volume |
289 |
container_issue |
36 |
container_start_page |
24821 |
op_container_end_page |
24831 |
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1766393741596164096 |