Optimization of triploid induction by the use of 6-DMAP for the oyster Crassostrea gigas (Thunberg)
A novel method using 6-dimcthylaminopurinc (6-0MAP) has recently been demonstrated to be a good triploid inductor for bivalve molluscs. The effects of 6-DMAP concentration, timing of treatment after fertilization and treatment duration on survival and triploid induction of Crassostrea gigas (Thunber...
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ftarchimer:oai:archimer.ifremer.fr:3198 2023-05-15T15:57:40+02:00 Optimization of triploid induction by the use of 6-DMAP for the oyster Crassostrea gigas (Thunberg) Gerard, Andre Naciri, Yamama Peignon, Jean-marie Ledu, Christophe 1994 application/pdf https://archimer.ifremer.fr/doc/1994/publication-3198.pdf https://archimer.ifremer.fr/doc/00000/3198/ eng eng https://archimer.ifremer.fr/doc/1994/publication-3198.pdf https://archimer.ifremer.fr/doc/00000/3198/ info:eu-repo/semantics/openAccess restricted use Aquaculture & Fisheries Management, 1994 , Vol. 25 , P. 709-719 Genetic Triploid induction Crassostrea gigas 6 DMAP Triploid Oysters text Publication info:eu-repo/semantics/article 1994 ftarchimer 2021-09-23T20:15:12Z A novel method using 6-dimcthylaminopurinc (6-0MAP) has recently been demonstrated to be a good triploid inductor for bivalve molluscs. The effects of 6-DMAP concentration, timing of treatment after fertilization and treatment duration on survival and triploid induction of Crassostrea gigas (Thunberg) embryos were examined. An influence of sperm concentration was also detected and discussed. Survival to D-stage was inversely related to 6-DMAP concentration and the percentage of triploids was shown to be 6-DMAP dose dependent. Two optimal moments for treatment application were determined corresponding to extrusions of the first and second polar bodies. Optimal treatment of 450 µmol/l 6-DMAP beginning 15 min after fertilization over a 10 min period yielded mean survival to D-stage of 64% with a mean of 85% triploid production. As a comparison, a sample treated with 1mg/l of cytochalasin B (CB), 20 min after fertilization over a 15 min period, yielded a mean survival to D-stage of only 36% with a mean of 95% triploid production. The advantages of 6-DMAP over CB arc clearly identified: this chemical is not carcinogenic, cheaper than cytochalasin B and much easier to use as it is water soluble Article in Journal/Newspaper Crassostrea gigas Archimer (Archive Institutionnelle de l'Ifremer - Institut français de recherche pour l'exploitation de la mer) |
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Open Polar |
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Archimer (Archive Institutionnelle de l'Ifremer - Institut français de recherche pour l'exploitation de la mer) |
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ftarchimer |
language |
English |
topic |
Genetic Triploid induction Crassostrea gigas 6 DMAP Triploid Oysters |
spellingShingle |
Genetic Triploid induction Crassostrea gigas 6 DMAP Triploid Oysters Gerard, Andre Naciri, Yamama Peignon, Jean-marie Ledu, Christophe Optimization of triploid induction by the use of 6-DMAP for the oyster Crassostrea gigas (Thunberg) |
topic_facet |
Genetic Triploid induction Crassostrea gigas 6 DMAP Triploid Oysters |
description |
A novel method using 6-dimcthylaminopurinc (6-0MAP) has recently been demonstrated to be a good triploid inductor for bivalve molluscs. The effects of 6-DMAP concentration, timing of treatment after fertilization and treatment duration on survival and triploid induction of Crassostrea gigas (Thunberg) embryos were examined. An influence of sperm concentration was also detected and discussed. Survival to D-stage was inversely related to 6-DMAP concentration and the percentage of triploids was shown to be 6-DMAP dose dependent. Two optimal moments for treatment application were determined corresponding to extrusions of the first and second polar bodies. Optimal treatment of 450 µmol/l 6-DMAP beginning 15 min after fertilization over a 10 min period yielded mean survival to D-stage of 64% with a mean of 85% triploid production. As a comparison, a sample treated with 1mg/l of cytochalasin B (CB), 20 min after fertilization over a 15 min period, yielded a mean survival to D-stage of only 36% with a mean of 95% triploid production. The advantages of 6-DMAP over CB arc clearly identified: this chemical is not carcinogenic, cheaper than cytochalasin B and much easier to use as it is water soluble |
format |
Article in Journal/Newspaper |
author |
Gerard, Andre Naciri, Yamama Peignon, Jean-marie Ledu, Christophe |
author_facet |
Gerard, Andre Naciri, Yamama Peignon, Jean-marie Ledu, Christophe |
author_sort |
Gerard, Andre |
title |
Optimization of triploid induction by the use of 6-DMAP for the oyster Crassostrea gigas (Thunberg) |
title_short |
Optimization of triploid induction by the use of 6-DMAP for the oyster Crassostrea gigas (Thunberg) |
title_full |
Optimization of triploid induction by the use of 6-DMAP for the oyster Crassostrea gigas (Thunberg) |
title_fullStr |
Optimization of triploid induction by the use of 6-DMAP for the oyster Crassostrea gigas (Thunberg) |
title_full_unstemmed |
Optimization of triploid induction by the use of 6-DMAP for the oyster Crassostrea gigas (Thunberg) |
title_sort |
optimization of triploid induction by the use of 6-dmap for the oyster crassostrea gigas (thunberg) |
publishDate |
1994 |
url |
https://archimer.ifremer.fr/doc/1994/publication-3198.pdf https://archimer.ifremer.fr/doc/00000/3198/ |
genre |
Crassostrea gigas |
genre_facet |
Crassostrea gigas |
op_source |
Aquaculture & Fisheries Management, 1994 , Vol. 25 , P. 709-719 |
op_relation |
https://archimer.ifremer.fr/doc/1994/publication-3198.pdf https://archimer.ifremer.fr/doc/00000/3198/ |
op_rights |
info:eu-repo/semantics/openAccess restricted use |
_version_ |
1766393339124383744 |