Functional Characterization of a short neuropeptide F-related receptor in a Lophotrochozoa, the mollusk Crassostrea gigas
Members of the short neuropeptide F (sNPF) family of peptides and their cognate receptors play key roles in a variety of physiological processes in arthropods. In silico screening of GigasDatabase, a specific expressed sequence tag database from the Pacific oyster Crassostrea gigas, resulted in the...
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Online Access: | https://archimer.ifremer.fr/doc/00207/31777/30188.pdf https://doi.org/10.1242/jeb.104067 https://archimer.ifremer.fr/doc/00207/31777/ |
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ftarchimer:oai:archimer.ifremer.fr:31777 2023-05-15T15:57:46+02:00 Functional Characterization of a short neuropeptide F-related receptor in a Lophotrochozoa, the mollusk Crassostrea gigas Bigot, Laetitia Beets, Isabel Dubos, Marie-pierre Boudry, Pierre Schoofs, Liliane Favrel, Pascal 2014-08 application/pdf https://archimer.ifremer.fr/doc/00207/31777/30188.pdf https://doi.org/10.1242/jeb.104067 https://archimer.ifremer.fr/doc/00207/31777/ eng eng Company Of Biologists Ltd https://archimer.ifremer.fr/doc/00207/31777/30188.pdf doi:10.1242/jeb.104067 https://archimer.ifremer.fr/doc/00207/31777/ 2014. Published by The Company of Biologists Ltd info:eu-repo/semantics/openAccess restricted use Journal Of Experimental Biology (0022-0949) (Company Of Biologists Ltd), 2014-08 , Vol. 217 , N. 16 , P. 2974-2982 Oyster Crassostrea gigas sNPF Energy metabolism Reproduction Starvation text Publication info:eu-repo/semantics/article 2014 ftarchimer https://doi.org/10.1242/jeb.104067 2021-09-23T20:24:50Z Members of the short neuropeptide F (sNPF) family of peptides and their cognate receptors play key roles in a variety of physiological processes in arthropods. In silico screening of GigasDatabase, a specific expressed sequence tag database from the Pacific oyster Crassostrea gigas, resulted in the identification of a receptor (CgsNPFR-like) phylogenetically closely related to sNPF receptors (sNPFRs) of insects. A reverse endocrinology approach was undertaken to identify the peptide ligand(s) of this orphan receptor. Though structurally distinct from insect sNPFs, three RFamide peptides derived from the same precursor, i.e. GSLFRFamide, SSLFRFamide and GALFRFamide, specifically activate the receptor in a dose-dependent manner, with respective EC50 values (halfmaximal effective concentrations) of 1.1, 2.1 and 4.1 µmol l-1. We found that both Cg-sNPFR-like receptor and LFRFamide encoding transcripts are expressed in the oyster central nervous system and in other tissues as well, albeit at lower levels. Mass spectrometry analysis confirmed the wide distribution of LFRFamide mature peptides in several central and peripheral tissues. The Cg-sNPFRlike receptor was more abundantly expressed in ganglia of females than of males, and upregulated in starved oysters. In the gonad area, highest receptor gene expression occurred at the start of gametogenesis, when storage activity is maximal. Our results suggest that signaling of LFRFamide peptides through the CgsNPFR-like receptor might play a role in the coordination of nutrition, energy storage and metabolism in C. gigas, possibly by promoting storage at the expense of reproduction. Article in Journal/Newspaper Crassostrea gigas Pacific oyster Archimer (Archive Institutionnelle de l'Ifremer - Institut français de recherche pour l'exploitation de la mer) Pacific Journal of Experimental Biology |
institution |
Open Polar |
collection |
Archimer (Archive Institutionnelle de l'Ifremer - Institut français de recherche pour l'exploitation de la mer) |
op_collection_id |
ftarchimer |
language |
English |
topic |
Oyster Crassostrea gigas sNPF Energy metabolism Reproduction Starvation |
spellingShingle |
Oyster Crassostrea gigas sNPF Energy metabolism Reproduction Starvation Bigot, Laetitia Beets, Isabel Dubos, Marie-pierre Boudry, Pierre Schoofs, Liliane Favrel, Pascal Functional Characterization of a short neuropeptide F-related receptor in a Lophotrochozoa, the mollusk Crassostrea gigas |
topic_facet |
Oyster Crassostrea gigas sNPF Energy metabolism Reproduction Starvation |
description |
Members of the short neuropeptide F (sNPF) family of peptides and their cognate receptors play key roles in a variety of physiological processes in arthropods. In silico screening of GigasDatabase, a specific expressed sequence tag database from the Pacific oyster Crassostrea gigas, resulted in the identification of a receptor (CgsNPFR-like) phylogenetically closely related to sNPF receptors (sNPFRs) of insects. A reverse endocrinology approach was undertaken to identify the peptide ligand(s) of this orphan receptor. Though structurally distinct from insect sNPFs, three RFamide peptides derived from the same precursor, i.e. GSLFRFamide, SSLFRFamide and GALFRFamide, specifically activate the receptor in a dose-dependent manner, with respective EC50 values (halfmaximal effective concentrations) of 1.1, 2.1 and 4.1 µmol l-1. We found that both Cg-sNPFR-like receptor and LFRFamide encoding transcripts are expressed in the oyster central nervous system and in other tissues as well, albeit at lower levels. Mass spectrometry analysis confirmed the wide distribution of LFRFamide mature peptides in several central and peripheral tissues. The Cg-sNPFRlike receptor was more abundantly expressed in ganglia of females than of males, and upregulated in starved oysters. In the gonad area, highest receptor gene expression occurred at the start of gametogenesis, when storage activity is maximal. Our results suggest that signaling of LFRFamide peptides through the CgsNPFR-like receptor might play a role in the coordination of nutrition, energy storage and metabolism in C. gigas, possibly by promoting storage at the expense of reproduction. |
format |
Article in Journal/Newspaper |
author |
Bigot, Laetitia Beets, Isabel Dubos, Marie-pierre Boudry, Pierre Schoofs, Liliane Favrel, Pascal |
author_facet |
Bigot, Laetitia Beets, Isabel Dubos, Marie-pierre Boudry, Pierre Schoofs, Liliane Favrel, Pascal |
author_sort |
Bigot, Laetitia |
title |
Functional Characterization of a short neuropeptide F-related receptor in a Lophotrochozoa, the mollusk Crassostrea gigas |
title_short |
Functional Characterization of a short neuropeptide F-related receptor in a Lophotrochozoa, the mollusk Crassostrea gigas |
title_full |
Functional Characterization of a short neuropeptide F-related receptor in a Lophotrochozoa, the mollusk Crassostrea gigas |
title_fullStr |
Functional Characterization of a short neuropeptide F-related receptor in a Lophotrochozoa, the mollusk Crassostrea gigas |
title_full_unstemmed |
Functional Characterization of a short neuropeptide F-related receptor in a Lophotrochozoa, the mollusk Crassostrea gigas |
title_sort |
functional characterization of a short neuropeptide f-related receptor in a lophotrochozoa, the mollusk crassostrea gigas |
publisher |
Company Of Biologists Ltd |
publishDate |
2014 |
url |
https://archimer.ifremer.fr/doc/00207/31777/30188.pdf https://doi.org/10.1242/jeb.104067 https://archimer.ifremer.fr/doc/00207/31777/ |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
Journal Of Experimental Biology (0022-0949) (Company Of Biologists Ltd), 2014-08 , Vol. 217 , N. 16 , P. 2974-2982 |
op_relation |
https://archimer.ifremer.fr/doc/00207/31777/30188.pdf doi:10.1242/jeb.104067 https://archimer.ifremer.fr/doc/00207/31777/ |
op_rights |
2014. Published by The Company of Biologists Ltd info:eu-repo/semantics/openAccess restricted use |
op_doi |
https://doi.org/10.1242/jeb.104067 |
container_title |
Journal of Experimental Biology |
_version_ |
1766393468882518016 |