Proteomic signatures of the oyster metabolic response to herpesvirus OsHV-1 μVar infection
Pacific oyster Crassostrea gigas were inoculated with OsHV-1 at low load (control) or high load (challenged) to better understand the pathogenesis of ostreid herpesvirus 1 (OsHV-1 μVar) and to determine which metabolic pathways might be affected during infection. Animals were sampled for proteomic a...
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Online Access: | https://archimer.ifremer.fr/doc/00199/31035/29439.pdf https://doi.org/10.1016/j.jprot.2014.06.030 https://archimer.ifremer.fr/doc/00199/31035/ |
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ftarchimer:oai:archimer.ifremer.fr:31035 2023-05-15T15:57:50+02:00 Proteomic signatures of the oyster metabolic response to herpesvirus OsHV-1 μVar infection Corporeau, Charlotte Tamayo, David Pernet, Fabrice Quere, Claudie Madec, Stephanie 2014-09-23 application/pdf https://archimer.ifremer.fr/doc/00199/31035/29439.pdf https://doi.org/10.1016/j.jprot.2014.06.030 https://archimer.ifremer.fr/doc/00199/31035/ eng eng Elsevier Science Bv https://archimer.ifremer.fr/doc/00199/31035/29439.pdf doi:10.1016/j.jprot.2014.06.030 https://archimer.ifremer.fr/doc/00199/31035/ 2014 Elsevier B.V. All rights reserved. info:eu-repo/semantics/openAccess restricted use Journal Of Proteomics (1874-3919) (Elsevier Science Bv), 2014-09-23 , Vol. 109 , P. 176-187 Marine bivalves Crassostrea gigas Herpesvirus Metabolism Warburg text Publication info:eu-repo/semantics/article 2014 ftarchimer https://doi.org/10.1016/j.jprot.2014.06.030 2021-09-23T20:24:41Z Pacific oyster Crassostrea gigas were inoculated with OsHV-1 at low load (control) or high load (challenged) to better understand the pathogenesis of ostreid herpesvirus 1 (OsHV-1 μVar) and to determine which metabolic pathways might be affected during infection. Animals were sampled for proteomic analysis two days post-injection, at the same time as OsHV-1 initiated an intense replication phase in challenged oysters. Twenty-five abundant protein spots that showed a marked change in accumulated levels were identified using a two-dimensional electrophoresis (2-DE) proteomic approach. Overall, these proteins are involved in cytoskeleton organization, protein turnover, induction of stress signals, signalling pathways and energy metabolism. Challenged oysters exhibited an increased glycolysis and VDAC accumulation, which reflect a “Warburg effect” as initially reported in cancer cells and more recently in shrimp infected with virus. The results presented here should be useful for identifying potential biomarkers of disease resistance and developing antiviral measures. Biological significance This study is the first 2-DE proteomic analysis dedicated to the pathogenesis of ostreid herpesvirus 1 (OsHV-1 μVar) in oyster Crassostrea gigas, the most important bivalve produced in the world. OsHV-1 has affected oysters every year since 2008. All the proteins identified in this paper are key targets involved in OsHV-1 infection processes. We presented evidence that the metabolic changes during infection in oyster somehow resemble the Warburg effect occurring in cancer cells. This work constitutes a real advance in the comprehension of the host metabolic pathways affected during OsHV-1 disease. Overall, this work contributes to a better understanding of disease mortalities in aquatic ecosystems which could guide management actions to mitigate their impacts. Article in Journal/Newspaper Crassostrea gigas Pacific oyster Archimer (Archive Institutionnelle de l'Ifremer - Institut français de recherche pour l'exploitation de la mer) Pacific Journal of Proteomics 109 176 187 |
institution |
Open Polar |
collection |
Archimer (Archive Institutionnelle de l'Ifremer - Institut français de recherche pour l'exploitation de la mer) |
op_collection_id |
ftarchimer |
language |
English |
topic |
Marine bivalves Crassostrea gigas Herpesvirus Metabolism Warburg |
spellingShingle |
Marine bivalves Crassostrea gigas Herpesvirus Metabolism Warburg Corporeau, Charlotte Tamayo, David Pernet, Fabrice Quere, Claudie Madec, Stephanie Proteomic signatures of the oyster metabolic response to herpesvirus OsHV-1 μVar infection |
topic_facet |
Marine bivalves Crassostrea gigas Herpesvirus Metabolism Warburg |
description |
Pacific oyster Crassostrea gigas were inoculated with OsHV-1 at low load (control) or high load (challenged) to better understand the pathogenesis of ostreid herpesvirus 1 (OsHV-1 μVar) and to determine which metabolic pathways might be affected during infection. Animals were sampled for proteomic analysis two days post-injection, at the same time as OsHV-1 initiated an intense replication phase in challenged oysters. Twenty-five abundant protein spots that showed a marked change in accumulated levels were identified using a two-dimensional electrophoresis (2-DE) proteomic approach. Overall, these proteins are involved in cytoskeleton organization, protein turnover, induction of stress signals, signalling pathways and energy metabolism. Challenged oysters exhibited an increased glycolysis and VDAC accumulation, which reflect a “Warburg effect” as initially reported in cancer cells and more recently in shrimp infected with virus. The results presented here should be useful for identifying potential biomarkers of disease resistance and developing antiviral measures. Biological significance This study is the first 2-DE proteomic analysis dedicated to the pathogenesis of ostreid herpesvirus 1 (OsHV-1 μVar) in oyster Crassostrea gigas, the most important bivalve produced in the world. OsHV-1 has affected oysters every year since 2008. All the proteins identified in this paper are key targets involved in OsHV-1 infection processes. We presented evidence that the metabolic changes during infection in oyster somehow resemble the Warburg effect occurring in cancer cells. This work constitutes a real advance in the comprehension of the host metabolic pathways affected during OsHV-1 disease. Overall, this work contributes to a better understanding of disease mortalities in aquatic ecosystems which could guide management actions to mitigate their impacts. |
format |
Article in Journal/Newspaper |
author |
Corporeau, Charlotte Tamayo, David Pernet, Fabrice Quere, Claudie Madec, Stephanie |
author_facet |
Corporeau, Charlotte Tamayo, David Pernet, Fabrice Quere, Claudie Madec, Stephanie |
author_sort |
Corporeau, Charlotte |
title |
Proteomic signatures of the oyster metabolic response to herpesvirus OsHV-1 μVar infection |
title_short |
Proteomic signatures of the oyster metabolic response to herpesvirus OsHV-1 μVar infection |
title_full |
Proteomic signatures of the oyster metabolic response to herpesvirus OsHV-1 μVar infection |
title_fullStr |
Proteomic signatures of the oyster metabolic response to herpesvirus OsHV-1 μVar infection |
title_full_unstemmed |
Proteomic signatures of the oyster metabolic response to herpesvirus OsHV-1 μVar infection |
title_sort |
proteomic signatures of the oyster metabolic response to herpesvirus oshv-1 μvar infection |
publisher |
Elsevier Science Bv |
publishDate |
2014 |
url |
https://archimer.ifremer.fr/doc/00199/31035/29439.pdf https://doi.org/10.1016/j.jprot.2014.06.030 https://archimer.ifremer.fr/doc/00199/31035/ |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
Journal Of Proteomics (1874-3919) (Elsevier Science Bv), 2014-09-23 , Vol. 109 , P. 176-187 |
op_relation |
https://archimer.ifremer.fr/doc/00199/31035/29439.pdf doi:10.1016/j.jprot.2014.06.030 https://archimer.ifremer.fr/doc/00199/31035/ |
op_rights |
2014 Elsevier B.V. All rights reserved. info:eu-repo/semantics/openAccess restricted use |
op_doi |
https://doi.org/10.1016/j.jprot.2014.06.030 |
container_title |
Journal of Proteomics |
container_volume |
109 |
container_start_page |
176 |
op_container_end_page |
187 |
_version_ |
1766393540418469888 |