In vitro modulation of reactive oxygen and nitrogen intermediate (ROI/RNI) production in Crassostrea gigas hemocytes
Bivalve hemocyte competence has been measured by quantifying functional characteristics, including reactive oxygen intermediate (ROI) production after activation with zymosan or phorbol myristate acetate (PMA). However, untreated oyster hemocytes also produce ROI and RNI (reactive nitrogen intermedi...
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ftarchimer:oai:archimer.ifremer.fr:3057 2023-05-15T15:57:59+02:00 In vitro modulation of reactive oxygen and nitrogen intermediate (ROI/RNI) production in Crassostrea gigas hemocytes Lambert, Christophe Soudant, Philippe Jegaden, Marine Delaporte, Maryse Labreuche, Yannick Moal, Jeanne Samain, Jean-francois 2007-09 application/pdf https://archimer.ifremer.fr/doc/2007/publication-3057.pdf https://doi.org/10.1016/j.aquaculture.2007.04.074 https://archimer.ifremer.fr/doc/00000/3057/ eng eng Elsevier https://archimer.ifremer.fr/doc/2007/publication-3057.pdf doi:10.1016/j.aquaculture.2007.04.074 https://archimer.ifremer.fr/doc/00000/3057/ 2007 Elsevier B.V. All rights reserved info:eu-repo/semantics/openAccess restricted use Aquaculture (0044-8486) (Elsevier), 2007-09 , Vol. 270 , N. 1-4 , P. 413-421 NO synthase NADPH oxidase Flow cytometry Reactive nitrogen intermediate RNI Reactive oxygen intermediate ROI Hemocytes Crassostrea gigas text Publication info:eu-repo/semantics/article 2007 ftarchimer https://doi.org/10.1016/j.aquaculture.2007.04.074 2021-09-23T20:15:08Z Bivalve hemocyte competence has been measured by quantifying functional characteristics, including reactive oxygen intermediate (ROI) production after activation with zymosan or phorbol myristate acetate (PMA). However, untreated oyster hemocytes also produce ROI and RNI (reactive nitrogen intermediates) after bleeding even if not stimulated by Zymosan or PMA. Extensive investigation of this parameter by flow cytometry showed that, in vitro, ROI/RNI production by untreated hemocytes maintained in seawater appeared to be independent of both bacterial burden in the serum and non-self particle phagocytosis. ROI/ RNI production in granulocytes was higher than in hyalinocytes and could be intensified when activated by zymosan but not by PMA. Both cell types used NADPH-oxidase- and NO-synthase-like pathways to produce these molecules; the NO-synthase pathway seemed relatively more dominant in hyalinocytes and NADPH-oxidase appeared more effective in granulocytes. These results provide new insights for interpreting the modulation of ROI/RNl production by untreated hemocytes shown by other studies, relative to environmental conditions or physiological status of the oysters. (c) 2007 Elsevier B.V. All rights reserved. Article in Journal/Newspaper Crassostrea gigas Archimer (Archive Institutionnelle de l'Ifremer - Institut français de recherche pour l'exploitation de la mer) Aquaculture 270 1-4 413 421 |
institution |
Open Polar |
collection |
Archimer (Archive Institutionnelle de l'Ifremer - Institut français de recherche pour l'exploitation de la mer) |
op_collection_id |
ftarchimer |
language |
English |
topic |
NO synthase NADPH oxidase Flow cytometry Reactive nitrogen intermediate RNI Reactive oxygen intermediate ROI Hemocytes Crassostrea gigas |
spellingShingle |
NO synthase NADPH oxidase Flow cytometry Reactive nitrogen intermediate RNI Reactive oxygen intermediate ROI Hemocytes Crassostrea gigas Lambert, Christophe Soudant, Philippe Jegaden, Marine Delaporte, Maryse Labreuche, Yannick Moal, Jeanne Samain, Jean-francois In vitro modulation of reactive oxygen and nitrogen intermediate (ROI/RNI) production in Crassostrea gigas hemocytes |
topic_facet |
NO synthase NADPH oxidase Flow cytometry Reactive nitrogen intermediate RNI Reactive oxygen intermediate ROI Hemocytes Crassostrea gigas |
description |
Bivalve hemocyte competence has been measured by quantifying functional characteristics, including reactive oxygen intermediate (ROI) production after activation with zymosan or phorbol myristate acetate (PMA). However, untreated oyster hemocytes also produce ROI and RNI (reactive nitrogen intermediates) after bleeding even if not stimulated by Zymosan or PMA. Extensive investigation of this parameter by flow cytometry showed that, in vitro, ROI/RNI production by untreated hemocytes maintained in seawater appeared to be independent of both bacterial burden in the serum and non-self particle phagocytosis. ROI/ RNI production in granulocytes was higher than in hyalinocytes and could be intensified when activated by zymosan but not by PMA. Both cell types used NADPH-oxidase- and NO-synthase-like pathways to produce these molecules; the NO-synthase pathway seemed relatively more dominant in hyalinocytes and NADPH-oxidase appeared more effective in granulocytes. These results provide new insights for interpreting the modulation of ROI/RNl production by untreated hemocytes shown by other studies, relative to environmental conditions or physiological status of the oysters. (c) 2007 Elsevier B.V. All rights reserved. |
format |
Article in Journal/Newspaper |
author |
Lambert, Christophe Soudant, Philippe Jegaden, Marine Delaporte, Maryse Labreuche, Yannick Moal, Jeanne Samain, Jean-francois |
author_facet |
Lambert, Christophe Soudant, Philippe Jegaden, Marine Delaporte, Maryse Labreuche, Yannick Moal, Jeanne Samain, Jean-francois |
author_sort |
Lambert, Christophe |
title |
In vitro modulation of reactive oxygen and nitrogen intermediate (ROI/RNI) production in Crassostrea gigas hemocytes |
title_short |
In vitro modulation of reactive oxygen and nitrogen intermediate (ROI/RNI) production in Crassostrea gigas hemocytes |
title_full |
In vitro modulation of reactive oxygen and nitrogen intermediate (ROI/RNI) production in Crassostrea gigas hemocytes |
title_fullStr |
In vitro modulation of reactive oxygen and nitrogen intermediate (ROI/RNI) production in Crassostrea gigas hemocytes |
title_full_unstemmed |
In vitro modulation of reactive oxygen and nitrogen intermediate (ROI/RNI) production in Crassostrea gigas hemocytes |
title_sort |
in vitro modulation of reactive oxygen and nitrogen intermediate (roi/rni) production in crassostrea gigas hemocytes |
publisher |
Elsevier |
publishDate |
2007 |
url |
https://archimer.ifremer.fr/doc/2007/publication-3057.pdf https://doi.org/10.1016/j.aquaculture.2007.04.074 https://archimer.ifremer.fr/doc/00000/3057/ |
genre |
Crassostrea gigas |
genre_facet |
Crassostrea gigas |
op_source |
Aquaculture (0044-8486) (Elsevier), 2007-09 , Vol. 270 , N. 1-4 , P. 413-421 |
op_relation |
https://archimer.ifremer.fr/doc/2007/publication-3057.pdf doi:10.1016/j.aquaculture.2007.04.074 https://archimer.ifremer.fr/doc/00000/3057/ |
op_rights |
2007 Elsevier B.V. All rights reserved info:eu-repo/semantics/openAccess restricted use |
op_doi |
https://doi.org/10.1016/j.aquaculture.2007.04.074 |
container_title |
Aquaculture |
container_volume |
270 |
container_issue |
1-4 |
container_start_page |
413 |
op_container_end_page |
421 |
_version_ |
1766393703404929024 |