Induced spawning of red drum, Sciaenops ocellatus: use of multivariate and univariate analysis methods in the search for side effects of LH-RHa treatments and ovarian development state upon spawn quality
The present work examines the usefulness of the combination of multivariate analyses and further one-way analysis of variance in the search for links between causes and effects in heterologous stimulation of spawning in fish. Stimulations with both 20 and 50 mu g . kg(-1) doses of [D Trp(6)] luteini...
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Online Access: | https://archimer.ifremer.fr/doc/00187/29875/28361.pdf https://doi.org/10.1016/S0990-7440(00)00137-6 https://archimer.ifremer.fr/doc/00187/29875/ |
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ftarchimer:oai:archimer.ifremer.fr:29875 2023-05-15T18:06:05+02:00 Induced spawning of red drum, Sciaenops ocellatus: use of multivariate and univariate analysis methods in the search for side effects of LH-RHa treatments and ovarian development state upon spawn quality Gardes, Lionel Villanove, Philippe Buchet, Vincent Fauvel, Christian 2000-01 application/pdf https://archimer.ifremer.fr/doc/00187/29875/28361.pdf https://doi.org/10.1016/S0990-7440(00)00137-6 https://archimer.ifremer.fr/doc/00187/29875/ eng eng Edp Sciences S A https://archimer.ifremer.fr/doc/00187/29875/28361.pdf doi:10.1016/S0990-7440(00)00137-6 https://archimer.ifremer.fr/doc/00187/29875/ 2000 Ifremer/Cnrs/Inra/Ird/Cemagref/Éditions scientifiques et médicales Elsevier SAS. All rights reserved info:eu-repo/semantics/openAccess restricted use Aquatic Living Resources (0990-7440) (Edp Sciences S A), 2000-01 , Vol. 13 , N. 1 , P. 19-27 Pisciculture induction de la ponte LH-RH analogues analyse multivariée qualité des oeufs développement ovocytaire ombrine Sciaenops ocellatus fish culture induced breeding multivariate analysis egg quality oocyte development red drum text Publication info:eu-repo/semantics/article 2000 ftarchimer https://doi.org/10.1016/S0990-7440(00)00137-6 2021-09-23T20:24:15Z The present work examines the usefulness of the combination of multivariate analyses and further one-way analysis of variance in the search for links between causes and effects in heterologous stimulation of spawning in fish. Stimulations with both 20 and 50 mu g . kg(-1) doses of [D Trp(6)] luteinizing hormone-releasing hormone analogue (LH-RHa) were effective in inducing and synchronizing spawning of red drum, Sciaenops ocellatus. Oocyte development monitoring using ovarian biopsy allowed treatment injections at different stages of maturation. The influence at stimulation time of female oocyte development state upon further development was shown. An interesting potential of second stimulation, leading to high quality spawns, was established and factors of success were identified. The best conditions for stimulation success were simultaneously late stage of oocyte development characterized by the beginning of oil droplet coalescence, high ovarian homogeneity quoted by the proportion of most developed oocytes in the biopsy sample, and low dose of hormone (20 mu g . kg(-1)) for both first and second stimulations. Ce travail examine l’intérêt de la combinaison d’analyses multivariées et de l’analyse de variance à un facteur pour la recherche de liens au sein d’expérimentations aquacoles faisant intervenir une diversité de causes et d’effets. L’injection de l’analogue [D Trp6] de la gonado-libérine (LH-RHa) est efficace aux doses de 20 et 50 íg×kg–1 pour provoquer et synchroniser les pontes chez l’ombrine, Sciaenops ocellatus. Un suivi du développement ovocytaire par biopsie ovarienne permet en outre de réaliser des injections à différents stades de maturation et l’influence du stade ovocytaire de la femelle, au moment de la stimulation sur la qualité de la ponte, est ainsi mise en évidence. Un potentiel intéressant suite à une seconde stimulation aboutissant à des pontes de qualité, est établi et des facteurs de réussite sont identifiés. Les meilleures conditions pour le succès de la stimulation sont simultanément : le dernier stade de développement ovocytaire, caractérisé par le début de coalescence de la gouttelette lipidique, la forte homogénéité ovarienne représentant la proportion des ovocytes les plus développés au sein de l’échantillon obtenu par biopsie, la faible dose hormonale (20 íg×kg–1) à la fois pour les premières et les secondes stimulations. Article in Journal/Newspaper Red drum Sciaenops ocellatus Archimer (Archive Institutionnelle de l'Ifremer - Institut français de recherche pour l'exploitation de la mer) Aquatic Living Resources 13 1 19 27 |
institution |
Open Polar |
collection |
Archimer (Archive Institutionnelle de l'Ifremer - Institut français de recherche pour l'exploitation de la mer) |
op_collection_id |
ftarchimer |
language |
English |
topic |
Pisciculture induction de la ponte LH-RH analogues analyse multivariée qualité des oeufs développement ovocytaire ombrine Sciaenops ocellatus fish culture induced breeding multivariate analysis egg quality oocyte development red drum |
spellingShingle |
Pisciculture induction de la ponte LH-RH analogues analyse multivariée qualité des oeufs développement ovocytaire ombrine Sciaenops ocellatus fish culture induced breeding multivariate analysis egg quality oocyte development red drum Gardes, Lionel Villanove, Philippe Buchet, Vincent Fauvel, Christian Induced spawning of red drum, Sciaenops ocellatus: use of multivariate and univariate analysis methods in the search for side effects of LH-RHa treatments and ovarian development state upon spawn quality |
topic_facet |
Pisciculture induction de la ponte LH-RH analogues analyse multivariée qualité des oeufs développement ovocytaire ombrine Sciaenops ocellatus fish culture induced breeding multivariate analysis egg quality oocyte development red drum |
description |
The present work examines the usefulness of the combination of multivariate analyses and further one-way analysis of variance in the search for links between causes and effects in heterologous stimulation of spawning in fish. Stimulations with both 20 and 50 mu g . kg(-1) doses of [D Trp(6)] luteinizing hormone-releasing hormone analogue (LH-RHa) were effective in inducing and synchronizing spawning of red drum, Sciaenops ocellatus. Oocyte development monitoring using ovarian biopsy allowed treatment injections at different stages of maturation. The influence at stimulation time of female oocyte development state upon further development was shown. An interesting potential of second stimulation, leading to high quality spawns, was established and factors of success were identified. The best conditions for stimulation success were simultaneously late stage of oocyte development characterized by the beginning of oil droplet coalescence, high ovarian homogeneity quoted by the proportion of most developed oocytes in the biopsy sample, and low dose of hormone (20 mu g . kg(-1)) for both first and second stimulations. Ce travail examine l’intérêt de la combinaison d’analyses multivariées et de l’analyse de variance à un facteur pour la recherche de liens au sein d’expérimentations aquacoles faisant intervenir une diversité de causes et d’effets. L’injection de l’analogue [D Trp6] de la gonado-libérine (LH-RHa) est efficace aux doses de 20 et 50 íg×kg–1 pour provoquer et synchroniser les pontes chez l’ombrine, Sciaenops ocellatus. Un suivi du développement ovocytaire par biopsie ovarienne permet en outre de réaliser des injections à différents stades de maturation et l’influence du stade ovocytaire de la femelle, au moment de la stimulation sur la qualité de la ponte, est ainsi mise en évidence. Un potentiel intéressant suite à une seconde stimulation aboutissant à des pontes de qualité, est établi et des facteurs de réussite sont identifiés. Les meilleures conditions pour le succès de la stimulation sont simultanément : le dernier stade de développement ovocytaire, caractérisé par le début de coalescence de la gouttelette lipidique, la forte homogénéité ovarienne représentant la proportion des ovocytes les plus développés au sein de l’échantillon obtenu par biopsie, la faible dose hormonale (20 íg×kg–1) à la fois pour les premières et les secondes stimulations. |
format |
Article in Journal/Newspaper |
author |
Gardes, Lionel Villanove, Philippe Buchet, Vincent Fauvel, Christian |
author_facet |
Gardes, Lionel Villanove, Philippe Buchet, Vincent Fauvel, Christian |
author_sort |
Gardes, Lionel |
title |
Induced spawning of red drum, Sciaenops ocellatus: use of multivariate and univariate analysis methods in the search for side effects of LH-RHa treatments and ovarian development state upon spawn quality |
title_short |
Induced spawning of red drum, Sciaenops ocellatus: use of multivariate and univariate analysis methods in the search for side effects of LH-RHa treatments and ovarian development state upon spawn quality |
title_full |
Induced spawning of red drum, Sciaenops ocellatus: use of multivariate and univariate analysis methods in the search for side effects of LH-RHa treatments and ovarian development state upon spawn quality |
title_fullStr |
Induced spawning of red drum, Sciaenops ocellatus: use of multivariate and univariate analysis methods in the search for side effects of LH-RHa treatments and ovarian development state upon spawn quality |
title_full_unstemmed |
Induced spawning of red drum, Sciaenops ocellatus: use of multivariate and univariate analysis methods in the search for side effects of LH-RHa treatments and ovarian development state upon spawn quality |
title_sort |
induced spawning of red drum, sciaenops ocellatus: use of multivariate and univariate analysis methods in the search for side effects of lh-rha treatments and ovarian development state upon spawn quality |
publisher |
Edp Sciences S A |
publishDate |
2000 |
url |
https://archimer.ifremer.fr/doc/00187/29875/28361.pdf https://doi.org/10.1016/S0990-7440(00)00137-6 https://archimer.ifremer.fr/doc/00187/29875/ |
genre |
Red drum Sciaenops ocellatus |
genre_facet |
Red drum Sciaenops ocellatus |
op_source |
Aquatic Living Resources (0990-7440) (Edp Sciences S A), 2000-01 , Vol. 13 , N. 1 , P. 19-27 |
op_relation |
https://archimer.ifremer.fr/doc/00187/29875/28361.pdf doi:10.1016/S0990-7440(00)00137-6 https://archimer.ifremer.fr/doc/00187/29875/ |
op_rights |
2000 Ifremer/Cnrs/Inra/Ird/Cemagref/Éditions scientifiques et médicales Elsevier SAS. All rights reserved info:eu-repo/semantics/openAccess restricted use |
op_doi |
https://doi.org/10.1016/S0990-7440(00)00137-6 |
container_title |
Aquatic Living Resources |
container_volume |
13 |
container_issue |
1 |
container_start_page |
19 |
op_container_end_page |
27 |
_version_ |
1766177661034430464 |