Demonstration of a true phenoloxidase activity and activation of a ProPO cascade in Pacific oyster, Crassostrea gigas (Thunberg) in vitro
The prophenoloxidase (ProPO) system is the origin of melanin production and is considered to be an innate defence mechanism in invertebrates. In different bivalve species, phenoloxidase (PO) is present in the haemolymph as an inactive form of ProPO. The present study focuses on the Pacific adult oys...
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Online Access: | https://archimer.ifremer.fr/doc/2007/publication-2926.pdf https://doi.org/10.1016/j.fsi.2006.06.014 https://archimer.ifremer.fr/doc/00000/2926/ |
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ftarchimer:oai:archimer.ifremer.fr:2926 2023-05-15T15:57:47+02:00 Demonstration of a true phenoloxidase activity and activation of a ProPO cascade in Pacific oyster, Crassostrea gigas (Thunberg) in vitro Hellio, Claire Bado Nilles, Anne Gagnaire, Beatrice Renault, Tristan Thomas-guyon, Helene 2007-04 application/pdf https://archimer.ifremer.fr/doc/2007/publication-2926.pdf https://doi.org/10.1016/j.fsi.2006.06.014 https://archimer.ifremer.fr/doc/00000/2926/ eng eng Elsevier https://archimer.ifremer.fr/doc/2007/publication-2926.pdf doi:10.1016/j.fsi.2006.06.014 https://archimer.ifremer.fr/doc/00000/2926/ info:eu-repo/semantics/openAccess restricted use Fish & Shellfish Immunology (1050-4648) (Elsevier), 2007-04 , Vol. 22 , N. 4 , P. 433-440 Prophenoloxidase Phenoloxidase Oyster Immunity Defence Crassostrea gigas text Publication info:eu-repo/semantics/article 2007 ftarchimer https://doi.org/10.1016/j.fsi.2006.06.014 2021-09-23T20:14:57Z The prophenoloxidase (ProPO) system is the origin of melanin production and is considered to be an innate defence mechanism in invertebrates. In different bivalve species, phenoloxidase (PO) is present in the haemolymph as an inactive form of ProPO. The present study focuses on the Pacific adult oyster, Crassostrea gigas, an economically important bivalve species along French coasts. The results indicate that many factors may inhibit the PO-like activity. These include: phenylthiourea (PTU), sodium diethylthiocarbamate (DETC), beta-mercaptoethanol and tropolone, which repressed the spontaneous PO activity. The activation of PO-like activity in C. gigas acellular fraction by lipopolysaccharide (LPS) involved participation of other factors, including at least one serine protease. PO was present as proPO in the acellular fraction of haemolymph and haemocytes of C. gigas and could be activated by an exogenous protease (trypsin-N-tosyl-L-phenylalanine chloromethyl ketone) when used at 1 g L-1. Treatment of acellular fractions with other modulators/activators namely LPS (1 g L-1), zymosan (0.6 g L-1) or laminarin (0.6 g L-1) also increased PO-like activity but to a less important way. The study demonstrated the evidence of a true phenoloxidase activity in Pacific oyster, C. gigas (Thunberg). The activation of a proPO system by non-self molecules suggests the role played by PO in vivo in the internal defence mechanisms. Understanding the activation of the ProPO system could enable the evaluation of the health of oyster stocks. (c) 2006 Published by Elsevier Ltd. Article in Journal/Newspaper Crassostrea gigas Pacific oyster Archimer (Archive Institutionnelle de l'Ifremer - Institut français de recherche pour l'exploitation de la mer) Pacific Fish & Shellfish Immunology 22 4 433 440 |
institution |
Open Polar |
collection |
Archimer (Archive Institutionnelle de l'Ifremer - Institut français de recherche pour l'exploitation de la mer) |
op_collection_id |
ftarchimer |
language |
English |
topic |
Prophenoloxidase Phenoloxidase Oyster Immunity Defence Crassostrea gigas |
spellingShingle |
Prophenoloxidase Phenoloxidase Oyster Immunity Defence Crassostrea gigas Hellio, Claire Bado Nilles, Anne Gagnaire, Beatrice Renault, Tristan Thomas-guyon, Helene Demonstration of a true phenoloxidase activity and activation of a ProPO cascade in Pacific oyster, Crassostrea gigas (Thunberg) in vitro |
topic_facet |
Prophenoloxidase Phenoloxidase Oyster Immunity Defence Crassostrea gigas |
description |
The prophenoloxidase (ProPO) system is the origin of melanin production and is considered to be an innate defence mechanism in invertebrates. In different bivalve species, phenoloxidase (PO) is present in the haemolymph as an inactive form of ProPO. The present study focuses on the Pacific adult oyster, Crassostrea gigas, an economically important bivalve species along French coasts. The results indicate that many factors may inhibit the PO-like activity. These include: phenylthiourea (PTU), sodium diethylthiocarbamate (DETC), beta-mercaptoethanol and tropolone, which repressed the spontaneous PO activity. The activation of PO-like activity in C. gigas acellular fraction by lipopolysaccharide (LPS) involved participation of other factors, including at least one serine protease. PO was present as proPO in the acellular fraction of haemolymph and haemocytes of C. gigas and could be activated by an exogenous protease (trypsin-N-tosyl-L-phenylalanine chloromethyl ketone) when used at 1 g L-1. Treatment of acellular fractions with other modulators/activators namely LPS (1 g L-1), zymosan (0.6 g L-1) or laminarin (0.6 g L-1) also increased PO-like activity but to a less important way. The study demonstrated the evidence of a true phenoloxidase activity in Pacific oyster, C. gigas (Thunberg). The activation of a proPO system by non-self molecules suggests the role played by PO in vivo in the internal defence mechanisms. Understanding the activation of the ProPO system could enable the evaluation of the health of oyster stocks. (c) 2006 Published by Elsevier Ltd. |
format |
Article in Journal/Newspaper |
author |
Hellio, Claire Bado Nilles, Anne Gagnaire, Beatrice Renault, Tristan Thomas-guyon, Helene |
author_facet |
Hellio, Claire Bado Nilles, Anne Gagnaire, Beatrice Renault, Tristan Thomas-guyon, Helene |
author_sort |
Hellio, Claire |
title |
Demonstration of a true phenoloxidase activity and activation of a ProPO cascade in Pacific oyster, Crassostrea gigas (Thunberg) in vitro |
title_short |
Demonstration of a true phenoloxidase activity and activation of a ProPO cascade in Pacific oyster, Crassostrea gigas (Thunberg) in vitro |
title_full |
Demonstration of a true phenoloxidase activity and activation of a ProPO cascade in Pacific oyster, Crassostrea gigas (Thunberg) in vitro |
title_fullStr |
Demonstration of a true phenoloxidase activity and activation of a ProPO cascade in Pacific oyster, Crassostrea gigas (Thunberg) in vitro |
title_full_unstemmed |
Demonstration of a true phenoloxidase activity and activation of a ProPO cascade in Pacific oyster, Crassostrea gigas (Thunberg) in vitro |
title_sort |
demonstration of a true phenoloxidase activity and activation of a propo cascade in pacific oyster, crassostrea gigas (thunberg) in vitro |
publisher |
Elsevier |
publishDate |
2007 |
url |
https://archimer.ifremer.fr/doc/2007/publication-2926.pdf https://doi.org/10.1016/j.fsi.2006.06.014 https://archimer.ifremer.fr/doc/00000/2926/ |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
Fish & Shellfish Immunology (1050-4648) (Elsevier), 2007-04 , Vol. 22 , N. 4 , P. 433-440 |
op_relation |
https://archimer.ifremer.fr/doc/2007/publication-2926.pdf doi:10.1016/j.fsi.2006.06.014 https://archimer.ifremer.fr/doc/00000/2926/ |
op_rights |
info:eu-repo/semantics/openAccess restricted use |
op_doi |
https://doi.org/10.1016/j.fsi.2006.06.014 |
container_title |
Fish & Shellfish Immunology |
container_volume |
22 |
container_issue |
4 |
container_start_page |
433 |
op_container_end_page |
440 |
_version_ |
1766393475430875136 |