Disturbance induced decoupling between host genetics and composition of the associated microbiome
Background Studies of oyster microbiomes have revealed that a limited number of microbes, including pathogens, can dominate microbial communities in host tissues such as gills and gut. Much of the bacterial diversity however remains underexplored and unexplained, although environmental conditions an...
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ftarchimer:oai:archimer.ifremer.fr:28202 2023-05-15T15:58:11+02:00 Disturbance induced decoupling between host genetics and composition of the associated microbiome Wegner, Karl Mathias Volkenborn, Nils Peter, Hannes Eiler, Alexander 2013-11 application/pdf https://archimer.ifremer.fr/doc/00171/28202/26430.pdf https://doi.org/10.1186/1471-2180-13-252 https://archimer.ifremer.fr/doc/00171/28202/ eng eng Biomed Central Ltd https://archimer.ifremer.fr/doc/00171/28202/26430.pdf doi:10.1186/1471-2180-13-252 https://archimer.ifremer.fr/doc/00171/28202/ 2013 Wegner et al.; licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. info:eu-repo/semantics/openAccess restricted use CC-BY Bmc Microbiology (1471-2180) (Biomed Central Ltd), 2013-11 , Vol. 13 , P. - Microbiota Population structure Stress Pathogen Biological invasion Pacific oyster Crassostrea gigas Vibrio Mycoplasma text Publication info:eu-repo/semantics/article 2013 ftarchimer https://doi.org/10.1186/1471-2180-13-252 2021-09-23T20:23:53Z Background Studies of oyster microbiomes have revealed that a limited number of microbes, including pathogens, can dominate microbial communities in host tissues such as gills and gut. Much of the bacterial diversity however remains underexplored and unexplained, although environmental conditions and host genetics have been implicated. We used 454 next generation 16S rRNA amplicon sequencing of individually tagged PCR reactions to explore the diversity of bacterial communities in gill tissue of the invasive Pacific oyster Crassostrea gigas stemming from genetically differentiated beds under ambient outdoor conditions and after a multifaceted disturbance treatment imposing stress on the host. Results While the gill associated microbial communities in oysters were dominated by few abundant taxa (i.e. Sphingomonas, Mycoplasma) the distribution of rare bacterial groups correlated to relatedness between the hosts under ambient conditions. Exposing the host to disturbance broke apart this relationship by removing rare phylotypes thereby reducing overall microbial diversity. Shifts in the microbiome composition in response to stress did not result in a net increase in genera known to contain potentially pathogenic strains. Conclusion The decrease in microbial diversity and the disassociation between population genetic structure of the hosts and their associated microbiome suggest that disturbance (i.e. stress) may play a significant role for the assembly of the natural microbiome. Such community shifts may in turn also feed back on the course of disease and the occurrence of mass mortality events in oyster populations. Article in Journal/Newspaper Crassostrea gigas Pacific oyster Archimer (Archive Institutionnelle de l'Ifremer - Institut français de recherche pour l'exploitation de la mer) Pacific BMC Microbiology 13 1 252 |
institution |
Open Polar |
collection |
Archimer (Archive Institutionnelle de l'Ifremer - Institut français de recherche pour l'exploitation de la mer) |
op_collection_id |
ftarchimer |
language |
English |
topic |
Microbiota Population structure Stress Pathogen Biological invasion Pacific oyster Crassostrea gigas Vibrio Mycoplasma |
spellingShingle |
Microbiota Population structure Stress Pathogen Biological invasion Pacific oyster Crassostrea gigas Vibrio Mycoplasma Wegner, Karl Mathias Volkenborn, Nils Peter, Hannes Eiler, Alexander Disturbance induced decoupling between host genetics and composition of the associated microbiome |
topic_facet |
Microbiota Population structure Stress Pathogen Biological invasion Pacific oyster Crassostrea gigas Vibrio Mycoplasma |
description |
Background Studies of oyster microbiomes have revealed that a limited number of microbes, including pathogens, can dominate microbial communities in host tissues such as gills and gut. Much of the bacterial diversity however remains underexplored and unexplained, although environmental conditions and host genetics have been implicated. We used 454 next generation 16S rRNA amplicon sequencing of individually tagged PCR reactions to explore the diversity of bacterial communities in gill tissue of the invasive Pacific oyster Crassostrea gigas stemming from genetically differentiated beds under ambient outdoor conditions and after a multifaceted disturbance treatment imposing stress on the host. Results While the gill associated microbial communities in oysters were dominated by few abundant taxa (i.e. Sphingomonas, Mycoplasma) the distribution of rare bacterial groups correlated to relatedness between the hosts under ambient conditions. Exposing the host to disturbance broke apart this relationship by removing rare phylotypes thereby reducing overall microbial diversity. Shifts in the microbiome composition in response to stress did not result in a net increase in genera known to contain potentially pathogenic strains. Conclusion The decrease in microbial diversity and the disassociation between population genetic structure of the hosts and their associated microbiome suggest that disturbance (i.e. stress) may play a significant role for the assembly of the natural microbiome. Such community shifts may in turn also feed back on the course of disease and the occurrence of mass mortality events in oyster populations. |
format |
Article in Journal/Newspaper |
author |
Wegner, Karl Mathias Volkenborn, Nils Peter, Hannes Eiler, Alexander |
author_facet |
Wegner, Karl Mathias Volkenborn, Nils Peter, Hannes Eiler, Alexander |
author_sort |
Wegner, Karl Mathias |
title |
Disturbance induced decoupling between host genetics and composition of the associated microbiome |
title_short |
Disturbance induced decoupling between host genetics and composition of the associated microbiome |
title_full |
Disturbance induced decoupling between host genetics and composition of the associated microbiome |
title_fullStr |
Disturbance induced decoupling between host genetics and composition of the associated microbiome |
title_full_unstemmed |
Disturbance induced decoupling between host genetics and composition of the associated microbiome |
title_sort |
disturbance induced decoupling between host genetics and composition of the associated microbiome |
publisher |
Biomed Central Ltd |
publishDate |
2013 |
url |
https://archimer.ifremer.fr/doc/00171/28202/26430.pdf https://doi.org/10.1186/1471-2180-13-252 https://archimer.ifremer.fr/doc/00171/28202/ |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
Bmc Microbiology (1471-2180) (Biomed Central Ltd), 2013-11 , Vol. 13 , P. - |
op_relation |
https://archimer.ifremer.fr/doc/00171/28202/26430.pdf doi:10.1186/1471-2180-13-252 https://archimer.ifremer.fr/doc/00171/28202/ |
op_rights |
2013 Wegner et al.; licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. info:eu-repo/semantics/openAccess restricted use |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.1186/1471-2180-13-252 |
container_title |
BMC Microbiology |
container_volume |
13 |
container_issue |
1 |
container_start_page |
252 |
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1766393915018051584 |