Sex-Specific Regulation of AMP-Activated Protein Kinase (AMPK) in the Pacific Oyster Crassostrea gigas
The hermaphrodite Pacific oyster Crassostrea gigas displays a high energy allocation to reproduction. We studied the expression of AMP-activated protein kinase (AMPK) during gametogenesis in the gonad and characterized the mRNA sequences of the AMPK subunits: the AMPK alpha mRNA sequence was previou...
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ftarchimer:oai:archimer.ifremer.fr:27911 2023-05-15T15:57:46+02:00 Sex-Specific Regulation of AMP-Activated Protein Kinase (AMPK) in the Pacific Oyster Crassostrea gigas Guevelou, Eric Huvet, Arnaud Galindo-sanchez, Clara E. Milan, Massimo Quillien, Virgile Daniel, Jean-yves Quere, Claudie Boudry, Pierre Corporeau, Charlotte 2013-10 application/pdf https://archimer.ifremer.fr/doc/00168/27911/26470.pdf https://doi.org/10.1095/biolreprod.113.109728 https://archimer.ifremer.fr/doc/00168/27911/ eng eng Soc Study Reproduction https://archimer.ifremer.fr/doc/00168/27911/26470.pdf doi:10.1095/biolreprod.113.109728 https://archimer.ifremer.fr/doc/00168/27911/ 2013 by the Society for the Study of Reproduction, Inc info:eu-repo/semantics/openAccess restricted use Biology Of Reproduction (0006-3363) (Soc Study Reproduction), 2013-10 , Vol. 89 , N. 4 , P. 1-15 AMP-activated protein kinase Crassostrea gigas gametogenesis marine bivalve reproduction text Publication info:eu-repo/semantics/article 2013 ftarchimer https://doi.org/10.1095/biolreprod.113.109728 2021-09-23T20:23:49Z The hermaphrodite Pacific oyster Crassostrea gigas displays a high energy allocation to reproduction. We studied the expression of AMP-activated protein kinase (AMPK) during gametogenesis in the gonad and characterized the mRNA sequences of the AMPK subunits: the AMPK alpha mRNA sequence was previously characterized; we identified AMPK beta, AMPK gamma, and mRNAs of putative AMPK-related targets following bioinformatics mining on existing genomic resources. We analyzed the mRNA expression of the AMPK alpha, beta, and gamma subunits in the gonads of male and female oysters through a reproductive cycle, and we quantified the mRNA expression of genes belonging to fatty acid and glucose metabolism. AMPK alpha mRNA levels were more abundant in males at the first stage of gametogenesis, when mitotic activity and the differentiation of germinal cells occur, and were always more abundant in males than in females. Some targets of fatty acid and glucose metabolism appeared to be correlated with the expression of AMPK subunits at the mRNA level. We then analyzed the sex-specific AMPK activity by measuring the phosphorylation of the catalytic AMPK alpha protein and its expression at the protein level. Both the amount of AMPK alpha protein and threonine 172 phosphorylation appeared to be almost totally inhibited in mature female gonads at stage 3, at the time when accumulation of reserves in oocytes was promoted, while it remained at a high level in mature spermatozoa. Its activation might play a sex-dependent role in the management of energy during gametogenesis in oyster. Article in Journal/Newspaper Crassostrea gigas Pacific oyster Archimer (Archive Institutionnelle de l'Ifremer - Institut français de recherche pour l'exploitation de la mer) Pacific Biology of Reproduction 89 4 |
institution |
Open Polar |
collection |
Archimer (Archive Institutionnelle de l'Ifremer - Institut français de recherche pour l'exploitation de la mer) |
op_collection_id |
ftarchimer |
language |
English |
topic |
AMP-activated protein kinase Crassostrea gigas gametogenesis marine bivalve reproduction |
spellingShingle |
AMP-activated protein kinase Crassostrea gigas gametogenesis marine bivalve reproduction Guevelou, Eric Huvet, Arnaud Galindo-sanchez, Clara E. Milan, Massimo Quillien, Virgile Daniel, Jean-yves Quere, Claudie Boudry, Pierre Corporeau, Charlotte Sex-Specific Regulation of AMP-Activated Protein Kinase (AMPK) in the Pacific Oyster Crassostrea gigas |
topic_facet |
AMP-activated protein kinase Crassostrea gigas gametogenesis marine bivalve reproduction |
description |
The hermaphrodite Pacific oyster Crassostrea gigas displays a high energy allocation to reproduction. We studied the expression of AMP-activated protein kinase (AMPK) during gametogenesis in the gonad and characterized the mRNA sequences of the AMPK subunits: the AMPK alpha mRNA sequence was previously characterized; we identified AMPK beta, AMPK gamma, and mRNAs of putative AMPK-related targets following bioinformatics mining on existing genomic resources. We analyzed the mRNA expression of the AMPK alpha, beta, and gamma subunits in the gonads of male and female oysters through a reproductive cycle, and we quantified the mRNA expression of genes belonging to fatty acid and glucose metabolism. AMPK alpha mRNA levels were more abundant in males at the first stage of gametogenesis, when mitotic activity and the differentiation of germinal cells occur, and were always more abundant in males than in females. Some targets of fatty acid and glucose metabolism appeared to be correlated with the expression of AMPK subunits at the mRNA level. We then analyzed the sex-specific AMPK activity by measuring the phosphorylation of the catalytic AMPK alpha protein and its expression at the protein level. Both the amount of AMPK alpha protein and threonine 172 phosphorylation appeared to be almost totally inhibited in mature female gonads at stage 3, at the time when accumulation of reserves in oocytes was promoted, while it remained at a high level in mature spermatozoa. Its activation might play a sex-dependent role in the management of energy during gametogenesis in oyster. |
format |
Article in Journal/Newspaper |
author |
Guevelou, Eric Huvet, Arnaud Galindo-sanchez, Clara E. Milan, Massimo Quillien, Virgile Daniel, Jean-yves Quere, Claudie Boudry, Pierre Corporeau, Charlotte |
author_facet |
Guevelou, Eric Huvet, Arnaud Galindo-sanchez, Clara E. Milan, Massimo Quillien, Virgile Daniel, Jean-yves Quere, Claudie Boudry, Pierre Corporeau, Charlotte |
author_sort |
Guevelou, Eric |
title |
Sex-Specific Regulation of AMP-Activated Protein Kinase (AMPK) in the Pacific Oyster Crassostrea gigas |
title_short |
Sex-Specific Regulation of AMP-Activated Protein Kinase (AMPK) in the Pacific Oyster Crassostrea gigas |
title_full |
Sex-Specific Regulation of AMP-Activated Protein Kinase (AMPK) in the Pacific Oyster Crassostrea gigas |
title_fullStr |
Sex-Specific Regulation of AMP-Activated Protein Kinase (AMPK) in the Pacific Oyster Crassostrea gigas |
title_full_unstemmed |
Sex-Specific Regulation of AMP-Activated Protein Kinase (AMPK) in the Pacific Oyster Crassostrea gigas |
title_sort |
sex-specific regulation of amp-activated protein kinase (ampk) in the pacific oyster crassostrea gigas |
publisher |
Soc Study Reproduction |
publishDate |
2013 |
url |
https://archimer.ifremer.fr/doc/00168/27911/26470.pdf https://doi.org/10.1095/biolreprod.113.109728 https://archimer.ifremer.fr/doc/00168/27911/ |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
Biology Of Reproduction (0006-3363) (Soc Study Reproduction), 2013-10 , Vol. 89 , N. 4 , P. 1-15 |
op_relation |
https://archimer.ifremer.fr/doc/00168/27911/26470.pdf doi:10.1095/biolreprod.113.109728 https://archimer.ifremer.fr/doc/00168/27911/ |
op_rights |
2013 by the Society for the Study of Reproduction, Inc info:eu-repo/semantics/openAccess restricted use |
op_doi |
https://doi.org/10.1095/biolreprod.113.109728 |
container_title |
Biology of Reproduction |
container_volume |
89 |
container_issue |
4 |
_version_ |
1766393468683288576 |