Poly I:C induces a protective antiviral immune response in the Pacific oyster (Crassostrea gigas) against subsequent challenge with Ostreid herpesvirus (OsHV-1 mu var)
In-vivo studies were carried out to investigate the protective effect of a synthetic viral analogue (poly I:C) against Ostreid herpes virus (OsHV-1 mu var). Pacific oysters (Crassostrea gigas) were immune-primed by intramuscular injection of 240 mu g of poly I:C or sterile seawater at 1 day prior to...
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Academic Press Ltd- Elsevier Science Ltd
2013
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Online Access: | https://archimer.ifremer.fr/doc/00152/26293/24739.pdf https://doi.org/10.1016/j.fsi.2013.04.051 https://archimer.ifremer.fr/doc/00152/26293/ |
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ftarchimer:oai:archimer.ifremer.fr:26293 2023-05-15T15:57:46+02:00 Poly I:C induces a protective antiviral immune response in the Pacific oyster (Crassostrea gigas) against subsequent challenge with Ostreid herpesvirus (OsHV-1 mu var) Green, Timothy Montagnani, Caroline 2013-08 application/pdf https://archimer.ifremer.fr/doc/00152/26293/24739.pdf https://doi.org/10.1016/j.fsi.2013.04.051 https://archimer.ifremer.fr/doc/00152/26293/ eng eng Academic Press Ltd- Elsevier Science Ltd https://archimer.ifremer.fr/doc/00152/26293/24739.pdf doi:10.1016/j.fsi.2013.04.051 https://archimer.ifremer.fr/doc/00152/26293/ 2013 Elsevier Ltd. All rights reserved. info:eu-repo/semantics/openAccess restricted use Fish & Shellfish Immunology (1050-4648) (Academic Press Ltd- Elsevier Science Ltd), 2013-08 , Vol. 35 , N. 2 , P. 382-388 Crassostrea gigas OsHV-1 Poly I:C Vibrio splendidus Interferon-like response text Publication info:eu-repo/semantics/article 2013 ftarchimer https://doi.org/10.1016/j.fsi.2013.04.051 2021-09-23T20:23:21Z In-vivo studies were carried out to investigate the protective effect of a synthetic viral analogue (poly I:C) against Ostreid herpes virus (OsHV-1 mu var). Pacific oysters (Crassostrea gigas) were immune-primed by intramuscular injection of 240 mu g of poly I:C or sterile seawater at 1 day prior to infection with OsHV-1 mu var. Poly I:C injection induced an antiviral state in C gigas as the percentage of viral-infected oysters at 48 h post infection was significantly lower in the poly I:C treatment (11%) compared to seawater controls (100%). In an additional experiment, we demonstrated that the protective role of poly I:C is reproducible and elicits a specific antiviral response as immune-priming with heat-killed Vibrio splendidus provided no protection against subsequent viral infection. In both experiments, genes homologous to a toll-like receptor (TLR), MyD88, interferon regulatory factor (IRF) and protein kinase R (PKR) were up-regulated in oysters immune-primed with poly I:C compared to seawater controls (p < 0.05). The MyD88, IRF and PKR genes were also significantly up-regulated in response to OsHV-1 mu var infection (p < 0.05), which is suggestive that they are implicated in the antiviral response of C gigas. Our results demonstrate that C gigas can recognise double-strand RNA to initiate an innate immune response that inhibits viral infection. The observed response has striking similarities to the hallmarks of the type-1 interferon response of vertebrates. Article in Journal/Newspaper Crassostrea gigas Pacific oyster Archimer (Archive Institutionnelle de l'Ifremer - Institut français de recherche pour l'exploitation de la mer) Pacific Fish & Shellfish Immunology 35 2 382 388 |
institution |
Open Polar |
collection |
Archimer (Archive Institutionnelle de l'Ifremer - Institut français de recherche pour l'exploitation de la mer) |
op_collection_id |
ftarchimer |
language |
English |
topic |
Crassostrea gigas OsHV-1 Poly I:C Vibrio splendidus Interferon-like response |
spellingShingle |
Crassostrea gigas OsHV-1 Poly I:C Vibrio splendidus Interferon-like response Green, Timothy Montagnani, Caroline Poly I:C induces a protective antiviral immune response in the Pacific oyster (Crassostrea gigas) against subsequent challenge with Ostreid herpesvirus (OsHV-1 mu var) |
topic_facet |
Crassostrea gigas OsHV-1 Poly I:C Vibrio splendidus Interferon-like response |
description |
In-vivo studies were carried out to investigate the protective effect of a synthetic viral analogue (poly I:C) against Ostreid herpes virus (OsHV-1 mu var). Pacific oysters (Crassostrea gigas) were immune-primed by intramuscular injection of 240 mu g of poly I:C or sterile seawater at 1 day prior to infection with OsHV-1 mu var. Poly I:C injection induced an antiviral state in C gigas as the percentage of viral-infected oysters at 48 h post infection was significantly lower in the poly I:C treatment (11%) compared to seawater controls (100%). In an additional experiment, we demonstrated that the protective role of poly I:C is reproducible and elicits a specific antiviral response as immune-priming with heat-killed Vibrio splendidus provided no protection against subsequent viral infection. In both experiments, genes homologous to a toll-like receptor (TLR), MyD88, interferon regulatory factor (IRF) and protein kinase R (PKR) were up-regulated in oysters immune-primed with poly I:C compared to seawater controls (p < 0.05). The MyD88, IRF and PKR genes were also significantly up-regulated in response to OsHV-1 mu var infection (p < 0.05), which is suggestive that they are implicated in the antiviral response of C gigas. Our results demonstrate that C gigas can recognise double-strand RNA to initiate an innate immune response that inhibits viral infection. The observed response has striking similarities to the hallmarks of the type-1 interferon response of vertebrates. |
format |
Article in Journal/Newspaper |
author |
Green, Timothy Montagnani, Caroline |
author_facet |
Green, Timothy Montagnani, Caroline |
author_sort |
Green, Timothy |
title |
Poly I:C induces a protective antiviral immune response in the Pacific oyster (Crassostrea gigas) against subsequent challenge with Ostreid herpesvirus (OsHV-1 mu var) |
title_short |
Poly I:C induces a protective antiviral immune response in the Pacific oyster (Crassostrea gigas) against subsequent challenge with Ostreid herpesvirus (OsHV-1 mu var) |
title_full |
Poly I:C induces a protective antiviral immune response in the Pacific oyster (Crassostrea gigas) against subsequent challenge with Ostreid herpesvirus (OsHV-1 mu var) |
title_fullStr |
Poly I:C induces a protective antiviral immune response in the Pacific oyster (Crassostrea gigas) against subsequent challenge with Ostreid herpesvirus (OsHV-1 mu var) |
title_full_unstemmed |
Poly I:C induces a protective antiviral immune response in the Pacific oyster (Crassostrea gigas) against subsequent challenge with Ostreid herpesvirus (OsHV-1 mu var) |
title_sort |
poly i:c induces a protective antiviral immune response in the pacific oyster (crassostrea gigas) against subsequent challenge with ostreid herpesvirus (oshv-1 mu var) |
publisher |
Academic Press Ltd- Elsevier Science Ltd |
publishDate |
2013 |
url |
https://archimer.ifremer.fr/doc/00152/26293/24739.pdf https://doi.org/10.1016/j.fsi.2013.04.051 https://archimer.ifremer.fr/doc/00152/26293/ |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
Fish & Shellfish Immunology (1050-4648) (Academic Press Ltd- Elsevier Science Ltd), 2013-08 , Vol. 35 , N. 2 , P. 382-388 |
op_relation |
https://archimer.ifremer.fr/doc/00152/26293/24739.pdf doi:10.1016/j.fsi.2013.04.051 https://archimer.ifremer.fr/doc/00152/26293/ |
op_rights |
2013 Elsevier Ltd. All rights reserved. info:eu-repo/semantics/openAccess restricted use |
op_doi |
https://doi.org/10.1016/j.fsi.2013.04.051 |
container_title |
Fish & Shellfish Immunology |
container_volume |
35 |
container_issue |
2 |
container_start_page |
382 |
op_container_end_page |
388 |
_version_ |
1766393465905610752 |