Temperature influence on pathogen transmission and subsequent mortalities in juvenile Pacific oysters Crassostrea gigas
Since 2008, mass mortalities of 1-yr-old Crassostrea gigas associated with the ostreid herpesvirus OsHV-1 μVar have occurred along all the coasts of France when seawater temperature reaches 16 to 17°C. The present study aimed to characterize the effect of temperature on oyster survival in combinatio...
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ftarchimer:oai:archimer.ifremer.fr:25891 2023-05-15T15:58:26+02:00 Temperature influence on pathogen transmission and subsequent mortalities in juvenile Pacific oysters Crassostrea gigas Petton, Bruno Pernet, Fabrice Robert, Rene Boudry, Pierre 2013 application/pdf https://archimer.ifremer.fr/doc/00148/25891/23912.pdf https://doi.org/10.3354/aei00070 https://archimer.ifremer.fr/doc/00148/25891/ eng eng Inter-research https://archimer.ifremer.fr/doc/00148/25891/23912.pdf doi:10.3354/aei00070 https://archimer.ifremer.fr/doc/00148/25891/ The authors 2013. Open Access under Creative Commons by Attribution Licence. Use, distribution and reproduction are un - restricted. Authors and original publication must be credited. info:eu-repo/semantics/openAccess restricted use CC-BY Aquaculture Environment Interactions (1869-215X) (Inter-research), 2013 , Vol. 3 , N. 3 , P. 257-273 Juvenile oyster Pathology Ostreid herpesvirus 1 Survival Temperature Vibrios text Publication info:eu-repo/semantics/article 2013 ftarchimer https://doi.org/10.3354/aei00070 2021-09-23T20:23:16Z Since 2008, mass mortalities of 1-yr-old Crassostrea gigas associated with the ostreid herpesvirus OsHV-1 μVar have occurred along all the coasts of France when seawater temperature reaches 16 to 17°C. The present study aimed to characterize the effect of temperature on oyster survival in combination with OsHV-1 DNA quantification by standard real-time PCR and total vibrio population levels in oyster tissues. To examine the effect of seawater temperature on disease transmission and related mortality of oysters, cohabitation experiments were conducted between healthy naïve oysters and oysters previously exposed to field conditions in areas where mortalities were occurring. Oysters initially maintained in controlled conditions (free of mortality and negative for OsHV-1), and then transferred to an area where high mortalities were occurring among farmed stocks, became infected with OsHV-1 and exhibited high loads of vibrios followed by significant mortalities. When previously exposed oysters were maintained indoors at 13.0°C for 40 d and then at 20.6°C, they exhibited no mortality, were negative for OsHV-1 detection, and did not transmit the disease to healthy oysters. Survival of previously exposed oysters maintained indoors at 8 temperatures ranging from 13.4 to 29.0°C varied from 25 to 48% and was negatively correlated with holding temperature. Concomitantly, survival of naïve cohabiting animals (62 to 98%) decreased with increasing seawater temperature until a plateau was reached between 16.2 and 21.9°C, and increased at higher temperatures. Therefore, the optimal temperature range for disease transmission from field-exposed to naïve animals was between 16.2 and 21.9°C. Our results suggest that a long-term period (40 d) at low temperature (13°C) may offer a method of mitigating mortalities in oysters that have been exposed to an infective environment. Article in Journal/Newspaper Crassostrea gigas Archimer (Archive Institutionnelle de l'Ifremer - Institut français de recherche pour l'exploitation de la mer) Pacific Aquaculture Environment Interactions 3 3 257 273 |
institution |
Open Polar |
collection |
Archimer (Archive Institutionnelle de l'Ifremer - Institut français de recherche pour l'exploitation de la mer) |
op_collection_id |
ftarchimer |
language |
English |
topic |
Juvenile oyster Pathology Ostreid herpesvirus 1 Survival Temperature Vibrios |
spellingShingle |
Juvenile oyster Pathology Ostreid herpesvirus 1 Survival Temperature Vibrios Petton, Bruno Pernet, Fabrice Robert, Rene Boudry, Pierre Temperature influence on pathogen transmission and subsequent mortalities in juvenile Pacific oysters Crassostrea gigas |
topic_facet |
Juvenile oyster Pathology Ostreid herpesvirus 1 Survival Temperature Vibrios |
description |
Since 2008, mass mortalities of 1-yr-old Crassostrea gigas associated with the ostreid herpesvirus OsHV-1 μVar have occurred along all the coasts of France when seawater temperature reaches 16 to 17°C. The present study aimed to characterize the effect of temperature on oyster survival in combination with OsHV-1 DNA quantification by standard real-time PCR and total vibrio population levels in oyster tissues. To examine the effect of seawater temperature on disease transmission and related mortality of oysters, cohabitation experiments were conducted between healthy naïve oysters and oysters previously exposed to field conditions in areas where mortalities were occurring. Oysters initially maintained in controlled conditions (free of mortality and negative for OsHV-1), and then transferred to an area where high mortalities were occurring among farmed stocks, became infected with OsHV-1 and exhibited high loads of vibrios followed by significant mortalities. When previously exposed oysters were maintained indoors at 13.0°C for 40 d and then at 20.6°C, they exhibited no mortality, were negative for OsHV-1 detection, and did not transmit the disease to healthy oysters. Survival of previously exposed oysters maintained indoors at 8 temperatures ranging from 13.4 to 29.0°C varied from 25 to 48% and was negatively correlated with holding temperature. Concomitantly, survival of naïve cohabiting animals (62 to 98%) decreased with increasing seawater temperature until a plateau was reached between 16.2 and 21.9°C, and increased at higher temperatures. Therefore, the optimal temperature range for disease transmission from field-exposed to naïve animals was between 16.2 and 21.9°C. Our results suggest that a long-term period (40 d) at low temperature (13°C) may offer a method of mitigating mortalities in oysters that have been exposed to an infective environment. |
format |
Article in Journal/Newspaper |
author |
Petton, Bruno Pernet, Fabrice Robert, Rene Boudry, Pierre |
author_facet |
Petton, Bruno Pernet, Fabrice Robert, Rene Boudry, Pierre |
author_sort |
Petton, Bruno |
title |
Temperature influence on pathogen transmission and subsequent mortalities in juvenile Pacific oysters Crassostrea gigas |
title_short |
Temperature influence on pathogen transmission and subsequent mortalities in juvenile Pacific oysters Crassostrea gigas |
title_full |
Temperature influence on pathogen transmission and subsequent mortalities in juvenile Pacific oysters Crassostrea gigas |
title_fullStr |
Temperature influence on pathogen transmission and subsequent mortalities in juvenile Pacific oysters Crassostrea gigas |
title_full_unstemmed |
Temperature influence on pathogen transmission and subsequent mortalities in juvenile Pacific oysters Crassostrea gigas |
title_sort |
temperature influence on pathogen transmission and subsequent mortalities in juvenile pacific oysters crassostrea gigas |
publisher |
Inter-research |
publishDate |
2013 |
url |
https://archimer.ifremer.fr/doc/00148/25891/23912.pdf https://doi.org/10.3354/aei00070 https://archimer.ifremer.fr/doc/00148/25891/ |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas |
genre_facet |
Crassostrea gigas |
op_source |
Aquaculture Environment Interactions (1869-215X) (Inter-research), 2013 , Vol. 3 , N. 3 , P. 257-273 |
op_relation |
https://archimer.ifremer.fr/doc/00148/25891/23912.pdf doi:10.3354/aei00070 https://archimer.ifremer.fr/doc/00148/25891/ |
op_rights |
The authors 2013. Open Access under Creative Commons by Attribution Licence. Use, distribution and reproduction are un - restricted. Authors and original publication must be credited. info:eu-repo/semantics/openAccess restricted use |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.3354/aei00070 |
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Aquaculture Environment Interactions |
container_volume |
3 |
container_issue |
3 |
container_start_page |
257 |
op_container_end_page |
273 |
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