Pacific oyster (Crassostrea gigas) hemocyte are not affected by a mixture of pesticides in short-term in vitro assays
Pesticides are frequently detected in estuaries among the pollutants found in estuarine and coastal areas and may have major ecological consequences. They could endanger organism growth, reproduction or survival. In the context of high mortality outbreaks affecting Pacific oysters, Crassostrea gigas...
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ftarchimer:oai:archimer.ifremer.fr:25555 2023-05-15T15:57:46+02:00 Pacific oyster (Crassostrea gigas) hemocyte are not affected by a mixture of pesticides in short-term in vitro assays Moreau, Pierrick Burgeot, Thierry Renault, Tristan 2014-04 application/pdf https://archimer.ifremer.fr/doc/00144/25555/23700.pdf https://doi.org/10.1007/s11356-013-1931-3 https://archimer.ifremer.fr/doc/00144/25555/ eng eng Springer Heidelberg https://archimer.ifremer.fr/doc/00144/25555/23700.pdf doi:10.1007/s11356-013-1931-3 https://archimer.ifremer.fr/doc/00144/25555/ Springer-Verlag Berlin Heidelberg 2013 info:eu-repo/semantics/openAccess restricted use Environmental Science And Pollution Research (0944-1344) (Springer Heidelberg), 2014-04 , Vol. 21 , N. 7 , P. 4940-4949 Immunity Hemocytes Pesticides Pacific oyster Crassostrea gigas Flow cytometry Bivalve text Publication info:eu-repo/semantics/article 2014 ftarchimer https://doi.org/10.1007/s11356-013-1931-3 2021-09-23T20:23:16Z Pesticides are frequently detected in estuaries among the pollutants found in estuarine and coastal areas and may have major ecological consequences. They could endanger organism growth, reproduction or survival. In the context of high mortality outbreaks affecting Pacific oysters, Crassostrea gigas, in France since 2008, it appears of importance to determine the putative effects of pesticides on oyster susceptibility to infectious agents. Massive mortality outbreaks reported in this species, mainly in spring and summer, may suggest an important role played by the seasonal use of pesticides and freshwater input in estuarine areas where oyster farms are frequently located. To understand the impact of some pesticides detected in French waters, their effects on Pacific oyster hemocytes were studied through short-term in vitro experiments. Bivalve immunity is mainly supported by hemocytes eliminating pathogens by phagocytosis and producing compounds including lysosomal enzymes and antimicrobial molecules. In this study, oyster hemocytes were incubated with a mixture of 14 pesticides and metaldehyde alone, a molecule used to eliminate land mollusks. Hemocyte parameters including dead/alive cells, nonspecific esterase activities, intracytoplasmic calcium, lysosome number and activity and phagocytosis were monitored by flow cytometry. No significant effect of pesticides tested at different concentrations was reported on oyster hemocytes maintained in vitro for short-term periods in the present study. It could be assumed that these oyster cells were resistant to pesticide exposure in tested conditions and developing in vivo assays appears as necessary to better understand the effects of polluants on immune system in mollucks. Article in Journal/Newspaper Crassostrea gigas Pacific oyster Archimer (Archive Institutionnelle de l'Ifremer - Institut français de recherche pour l'exploitation de la mer) Pacific Environmental Science and Pollution Research 21 7 4940 4949 |
institution |
Open Polar |
collection |
Archimer (Archive Institutionnelle de l'Ifremer - Institut français de recherche pour l'exploitation de la mer) |
op_collection_id |
ftarchimer |
language |
English |
topic |
Immunity Hemocytes Pesticides Pacific oyster Crassostrea gigas Flow cytometry Bivalve |
spellingShingle |
Immunity Hemocytes Pesticides Pacific oyster Crassostrea gigas Flow cytometry Bivalve Moreau, Pierrick Burgeot, Thierry Renault, Tristan Pacific oyster (Crassostrea gigas) hemocyte are not affected by a mixture of pesticides in short-term in vitro assays |
topic_facet |
Immunity Hemocytes Pesticides Pacific oyster Crassostrea gigas Flow cytometry Bivalve |
description |
Pesticides are frequently detected in estuaries among the pollutants found in estuarine and coastal areas and may have major ecological consequences. They could endanger organism growth, reproduction or survival. In the context of high mortality outbreaks affecting Pacific oysters, Crassostrea gigas, in France since 2008, it appears of importance to determine the putative effects of pesticides on oyster susceptibility to infectious agents. Massive mortality outbreaks reported in this species, mainly in spring and summer, may suggest an important role played by the seasonal use of pesticides and freshwater input in estuarine areas where oyster farms are frequently located. To understand the impact of some pesticides detected in French waters, their effects on Pacific oyster hemocytes were studied through short-term in vitro experiments. Bivalve immunity is mainly supported by hemocytes eliminating pathogens by phagocytosis and producing compounds including lysosomal enzymes and antimicrobial molecules. In this study, oyster hemocytes were incubated with a mixture of 14 pesticides and metaldehyde alone, a molecule used to eliminate land mollusks. Hemocyte parameters including dead/alive cells, nonspecific esterase activities, intracytoplasmic calcium, lysosome number and activity and phagocytosis were monitored by flow cytometry. No significant effect of pesticides tested at different concentrations was reported on oyster hemocytes maintained in vitro for short-term periods in the present study. It could be assumed that these oyster cells were resistant to pesticide exposure in tested conditions and developing in vivo assays appears as necessary to better understand the effects of polluants on immune system in mollucks. |
format |
Article in Journal/Newspaper |
author |
Moreau, Pierrick Burgeot, Thierry Renault, Tristan |
author_facet |
Moreau, Pierrick Burgeot, Thierry Renault, Tristan |
author_sort |
Moreau, Pierrick |
title |
Pacific oyster (Crassostrea gigas) hemocyte are not affected by a mixture of pesticides in short-term in vitro assays |
title_short |
Pacific oyster (Crassostrea gigas) hemocyte are not affected by a mixture of pesticides in short-term in vitro assays |
title_full |
Pacific oyster (Crassostrea gigas) hemocyte are not affected by a mixture of pesticides in short-term in vitro assays |
title_fullStr |
Pacific oyster (Crassostrea gigas) hemocyte are not affected by a mixture of pesticides in short-term in vitro assays |
title_full_unstemmed |
Pacific oyster (Crassostrea gigas) hemocyte are not affected by a mixture of pesticides in short-term in vitro assays |
title_sort |
pacific oyster (crassostrea gigas) hemocyte are not affected by a mixture of pesticides in short-term in vitro assays |
publisher |
Springer Heidelberg |
publishDate |
2014 |
url |
https://archimer.ifremer.fr/doc/00144/25555/23700.pdf https://doi.org/10.1007/s11356-013-1931-3 https://archimer.ifremer.fr/doc/00144/25555/ |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
Environmental Science And Pollution Research (0944-1344) (Springer Heidelberg), 2014-04 , Vol. 21 , N. 7 , P. 4940-4949 |
op_relation |
https://archimer.ifremer.fr/doc/00144/25555/23700.pdf doi:10.1007/s11356-013-1931-3 https://archimer.ifremer.fr/doc/00144/25555/ |
op_rights |
Springer-Verlag Berlin Heidelberg 2013 info:eu-repo/semantics/openAccess restricted use |
op_doi |
https://doi.org/10.1007/s11356-013-1931-3 |
container_title |
Environmental Science and Pollution Research |
container_volume |
21 |
container_issue |
7 |
container_start_page |
4940 |
op_container_end_page |
4949 |
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1766393465733644288 |