A method for the use of the radiotracer 55Fe for microautoradiography and CARD-FISH of natural bacterial communities
Heterotrophic bacteria are key players in the biogeochemical cycle of iron (Fe) in the ocean, but the capability of different bacterial groups to access this micronutrient is ignored thus far. The aim of our study was to develop a protocol for the combined application of microautoradiography (MICRO)...
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Online Access: | https://archimer.ifremer.fr/doc/00143/25403/23555.pdf https://doi.org/10.1111/1574-6968.12022 https://archimer.ifremer.fr/doc/00143/25403/ |
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ftarchimer:oai:archimer.ifremer.fr:25403 2023-05-15T18:25:33+02:00 A method for the use of the radiotracer 55Fe for microautoradiography and CARD-FISH of natural bacterial communities Fourquez, Marion Obernosterer, Ingrid Blain, Stephane 2012-12 application/pdf https://archimer.ifremer.fr/doc/00143/25403/23555.pdf https://doi.org/10.1111/1574-6968.12022 https://archimer.ifremer.fr/doc/00143/25403/ eng eng Wiley-blackwell https://archimer.ifremer.fr/doc/00143/25403/23555.pdf doi:10.1111/1574-6968.12022 https://archimer.ifremer.fr/doc/00143/25403/ 2012 Federation of European Microbiological Societies Published by Blackwell Publishing Ltd. All rights reserved info:eu-repo/semantics/openAccess restricted use Fems Microbiology Letters (0378-1097) (Wiley-blackwell), 2012-12 , Vol. 337 , N. 2 , P. 132-139 microautoradiography 55Fe CARD-FISH marine bacteria text Publication info:eu-repo/semantics/article 2012 ftarchimer https://doi.org/10.1111/1574-6968.12022 2021-09-23T20:23:12Z Heterotrophic bacteria are key players in the biogeochemical cycle of iron (Fe) in the ocean, but the capability of different bacterial groups to access this micronutrient is ignored thus far. The aim of our study was to develop a protocol for the combined application of microautoradiography (MICRO) and catalyzed reporter depositionfluorescence in situ hybridization (CARD-FISH) using the radioisotope 55Fe. Among the different washing solutions tested, Ti-citrate-EDTA was the most efficient for the removal of extracellular 55Fe providing sufficiently low background values. We further demonstrate that the washing of cells with Ti-citrate-EDTA and the fixation with paraformaldehyde or formaldehyde do not induce leakage of intracellular 55Fe. Incubating natural bacterial communities collected from contrasting environments, the NW Mediterranean Sea and the Southern Ocean, with 55Fe revealed that 329% of bacterial cells were associated with silver grains. Combining microautoradiography with CARD-FISH, we demonstrate that the contribution of different bacterial groups to total 55Fe-incorporating cells was overall reflected by their relative contribution to abundance. An exception to this pattern was the proportionally higher contribution of Gammaproteobacteria, SAR86 and Alteromonas. Our study demonstrates the feasibility of MICRO-CARD-FISH using the radiotracer 55Fe and provides the first description of marine bacterial assemblages actively incorporating Fe. Article in Journal/Newspaper Southern Ocean Archimer (Archive Institutionnelle de l'Ifremer - Institut français de recherche pour l'exploitation de la mer) Southern Ocean FEMS Microbiology Letters 337 2 132 139 |
institution |
Open Polar |
collection |
Archimer (Archive Institutionnelle de l'Ifremer - Institut français de recherche pour l'exploitation de la mer) |
op_collection_id |
ftarchimer |
language |
English |
topic |
microautoradiography 55Fe CARD-FISH marine bacteria |
spellingShingle |
microautoradiography 55Fe CARD-FISH marine bacteria Fourquez, Marion Obernosterer, Ingrid Blain, Stephane A method for the use of the radiotracer 55Fe for microautoradiography and CARD-FISH of natural bacterial communities |
topic_facet |
microautoradiography 55Fe CARD-FISH marine bacteria |
description |
Heterotrophic bacteria are key players in the biogeochemical cycle of iron (Fe) in the ocean, but the capability of different bacterial groups to access this micronutrient is ignored thus far. The aim of our study was to develop a protocol for the combined application of microautoradiography (MICRO) and catalyzed reporter depositionfluorescence in situ hybridization (CARD-FISH) using the radioisotope 55Fe. Among the different washing solutions tested, Ti-citrate-EDTA was the most efficient for the removal of extracellular 55Fe providing sufficiently low background values. We further demonstrate that the washing of cells with Ti-citrate-EDTA and the fixation with paraformaldehyde or formaldehyde do not induce leakage of intracellular 55Fe. Incubating natural bacterial communities collected from contrasting environments, the NW Mediterranean Sea and the Southern Ocean, with 55Fe revealed that 329% of bacterial cells were associated with silver grains. Combining microautoradiography with CARD-FISH, we demonstrate that the contribution of different bacterial groups to total 55Fe-incorporating cells was overall reflected by their relative contribution to abundance. An exception to this pattern was the proportionally higher contribution of Gammaproteobacteria, SAR86 and Alteromonas. Our study demonstrates the feasibility of MICRO-CARD-FISH using the radiotracer 55Fe and provides the first description of marine bacterial assemblages actively incorporating Fe. |
format |
Article in Journal/Newspaper |
author |
Fourquez, Marion Obernosterer, Ingrid Blain, Stephane |
author_facet |
Fourquez, Marion Obernosterer, Ingrid Blain, Stephane |
author_sort |
Fourquez, Marion |
title |
A method for the use of the radiotracer 55Fe for microautoradiography and CARD-FISH of natural bacterial communities |
title_short |
A method for the use of the radiotracer 55Fe for microautoradiography and CARD-FISH of natural bacterial communities |
title_full |
A method for the use of the radiotracer 55Fe for microautoradiography and CARD-FISH of natural bacterial communities |
title_fullStr |
A method for the use of the radiotracer 55Fe for microautoradiography and CARD-FISH of natural bacterial communities |
title_full_unstemmed |
A method for the use of the radiotracer 55Fe for microautoradiography and CARD-FISH of natural bacterial communities |
title_sort |
method for the use of the radiotracer 55fe for microautoradiography and card-fish of natural bacterial communities |
publisher |
Wiley-blackwell |
publishDate |
2012 |
url |
https://archimer.ifremer.fr/doc/00143/25403/23555.pdf https://doi.org/10.1111/1574-6968.12022 https://archimer.ifremer.fr/doc/00143/25403/ |
geographic |
Southern Ocean |
geographic_facet |
Southern Ocean |
genre |
Southern Ocean |
genre_facet |
Southern Ocean |
op_source |
Fems Microbiology Letters (0378-1097) (Wiley-blackwell), 2012-12 , Vol. 337 , N. 2 , P. 132-139 |
op_relation |
https://archimer.ifremer.fr/doc/00143/25403/23555.pdf doi:10.1111/1574-6968.12022 https://archimer.ifremer.fr/doc/00143/25403/ |
op_rights |
2012 Federation of European Microbiological Societies Published by Blackwell Publishing Ltd. All rights reserved info:eu-repo/semantics/openAccess restricted use |
op_doi |
https://doi.org/10.1111/1574-6968.12022 |
container_title |
FEMS Microbiology Letters |
container_volume |
337 |
container_issue |
2 |
container_start_page |
132 |
op_container_end_page |
139 |
_version_ |
1766207087709257728 |