Paralytic Toxins Accumulation and Tissue Expression of alpha-Amylase and Lipase Genes in the Pacific Oyster Crassostrea gigas Fed with the Neurotoxic Dinoflagellate Alexandrium catenella
The pacific oyster Crassostrea gigas was experimentally exposed to the neurotoxic Alexandrium catenella and a non-producer of PSTs, Alexandrium tamarense (control algae), at concentrations corresponding to those observed during the blooming period. At fixed time intervals, from 0 to 48 h, we determi...
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ftarchimer:oai:archimer.ifremer.fr:24170 2023-05-15T15:57:44+02:00 Paralytic Toxins Accumulation and Tissue Expression of alpha-Amylase and Lipase Genes in the Pacific Oyster Crassostrea gigas Fed with the Neurotoxic Dinoflagellate Alexandrium catenella Rolland, Jean-luc Pelletier, Kevin Masseret, Estelle Rieuvilleneuve, Fabien Savar, Veronique Santini, Adrien Amzil, Zouher 2012-11 application/pdf https://archimer.ifremer.fr/doc/00130/24170/22172.pdf https://doi.org/10.3390/md10112519 https://archimer.ifremer.fr/doc/00130/24170/ eng eng Mdpi Ag https://archimer.ifremer.fr/doc/00130/24170/22172.pdf doi:10.3390/md10112519 https://archimer.ifremer.fr/doc/00130/24170/ 2012 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/). info:eu-repo/semantics/openAccess restricted use CC-BY Marine Drugs (1660-3397) (Mdpi Ag), 2012-11 , Vol. 10 , N. 11 , P. 2519-2534 Crassostrea gigas Alexandrium catenella PSP toxins digestion gene expression text Publication info:eu-repo/semantics/article 2012 ftarchimer https://doi.org/10.3390/md10112519 2021-09-23T20:22:42Z The pacific oyster Crassostrea gigas was experimentally exposed to the neurotoxic Alexandrium catenella and a non-producer of PSTs, Alexandrium tamarense (control algae), at concentrations corresponding to those observed during the blooming period. At fixed time intervals, from 0 to 48 h, we determined the clearance rate, the total filtered cells, the composition of the fecal ribbons, the profile of the PSP toxins and the variation of the expression of two a-amylase and triacylglecerol lipase precursor (TLP) genes through semi-quantitative RT-PCR. The results showed a significant decrease of the clearance rate of C. gigas fed with both Alexandrium species. However, from 29 to 48 h, the clearance rate and cell filtration activity increased only in oysters fed with A. tamarense. The toxin concentrations in the digestive gland rose above the sanitary threshold in less than 48 h of exposure and GTX6, a compound absent in A. catenella cells, accumulated. The a-amylase B gene expression level increased significantly in the time interval from 6 to 48 h in the digestive gland of oysters fed with A. tamarense, whereas the TLP gene transcript was significantly up-regulated in the digestive gland of oysters fed with the neurotoxic A. catenella. All together, these results suggest that the digestion capacity could be affected by PSP toxins. Article in Journal/Newspaper Crassostrea gigas Pacific oyster Archimer (Archive Institutionnelle de l'Ifremer - Institut français de recherche pour l'exploitation de la mer) Pacific Marine Drugs 10 12 2519 2534 |
institution |
Open Polar |
collection |
Archimer (Archive Institutionnelle de l'Ifremer - Institut français de recherche pour l'exploitation de la mer) |
op_collection_id |
ftarchimer |
language |
English |
topic |
Crassostrea gigas Alexandrium catenella PSP toxins digestion gene expression |
spellingShingle |
Crassostrea gigas Alexandrium catenella PSP toxins digestion gene expression Rolland, Jean-luc Pelletier, Kevin Masseret, Estelle Rieuvilleneuve, Fabien Savar, Veronique Santini, Adrien Amzil, Zouher Paralytic Toxins Accumulation and Tissue Expression of alpha-Amylase and Lipase Genes in the Pacific Oyster Crassostrea gigas Fed with the Neurotoxic Dinoflagellate Alexandrium catenella |
topic_facet |
Crassostrea gigas Alexandrium catenella PSP toxins digestion gene expression |
description |
The pacific oyster Crassostrea gigas was experimentally exposed to the neurotoxic Alexandrium catenella and a non-producer of PSTs, Alexandrium tamarense (control algae), at concentrations corresponding to those observed during the blooming period. At fixed time intervals, from 0 to 48 h, we determined the clearance rate, the total filtered cells, the composition of the fecal ribbons, the profile of the PSP toxins and the variation of the expression of two a-amylase and triacylglecerol lipase precursor (TLP) genes through semi-quantitative RT-PCR. The results showed a significant decrease of the clearance rate of C. gigas fed with both Alexandrium species. However, from 29 to 48 h, the clearance rate and cell filtration activity increased only in oysters fed with A. tamarense. The toxin concentrations in the digestive gland rose above the sanitary threshold in less than 48 h of exposure and GTX6, a compound absent in A. catenella cells, accumulated. The a-amylase B gene expression level increased significantly in the time interval from 6 to 48 h in the digestive gland of oysters fed with A. tamarense, whereas the TLP gene transcript was significantly up-regulated in the digestive gland of oysters fed with the neurotoxic A. catenella. All together, these results suggest that the digestion capacity could be affected by PSP toxins. |
format |
Article in Journal/Newspaper |
author |
Rolland, Jean-luc Pelletier, Kevin Masseret, Estelle Rieuvilleneuve, Fabien Savar, Veronique Santini, Adrien Amzil, Zouher |
author_facet |
Rolland, Jean-luc Pelletier, Kevin Masseret, Estelle Rieuvilleneuve, Fabien Savar, Veronique Santini, Adrien Amzil, Zouher |
author_sort |
Rolland, Jean-luc |
title |
Paralytic Toxins Accumulation and Tissue Expression of alpha-Amylase and Lipase Genes in the Pacific Oyster Crassostrea gigas Fed with the Neurotoxic Dinoflagellate Alexandrium catenella |
title_short |
Paralytic Toxins Accumulation and Tissue Expression of alpha-Amylase and Lipase Genes in the Pacific Oyster Crassostrea gigas Fed with the Neurotoxic Dinoflagellate Alexandrium catenella |
title_full |
Paralytic Toxins Accumulation and Tissue Expression of alpha-Amylase and Lipase Genes in the Pacific Oyster Crassostrea gigas Fed with the Neurotoxic Dinoflagellate Alexandrium catenella |
title_fullStr |
Paralytic Toxins Accumulation and Tissue Expression of alpha-Amylase and Lipase Genes in the Pacific Oyster Crassostrea gigas Fed with the Neurotoxic Dinoflagellate Alexandrium catenella |
title_full_unstemmed |
Paralytic Toxins Accumulation and Tissue Expression of alpha-Amylase and Lipase Genes in the Pacific Oyster Crassostrea gigas Fed with the Neurotoxic Dinoflagellate Alexandrium catenella |
title_sort |
paralytic toxins accumulation and tissue expression of alpha-amylase and lipase genes in the pacific oyster crassostrea gigas fed with the neurotoxic dinoflagellate alexandrium catenella |
publisher |
Mdpi Ag |
publishDate |
2012 |
url |
https://archimer.ifremer.fr/doc/00130/24170/22172.pdf https://doi.org/10.3390/md10112519 https://archimer.ifremer.fr/doc/00130/24170/ |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
Marine Drugs (1660-3397) (Mdpi Ag), 2012-11 , Vol. 10 , N. 11 , P. 2519-2534 |
op_relation |
https://archimer.ifremer.fr/doc/00130/24170/22172.pdf doi:10.3390/md10112519 https://archimer.ifremer.fr/doc/00130/24170/ |
op_rights |
2012 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/). info:eu-repo/semantics/openAccess restricted use |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.3390/md10112519 |
container_title |
Marine Drugs |
container_volume |
10 |
container_issue |
12 |
container_start_page |
2519 |
op_container_end_page |
2534 |
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1766393426184503296 |