Evidence for the involvement of pathogenic bacteria in summer mortalities of the Pacific oyster Crassostrea gigas
A study was conducted to investigate the involvement of bacteria in oyster mortalities during summer. Moribund and apparently healthy oysters were sampled during mortality events along the French coast and in rearing facilities, usually when temperature reached 19 degrees C or higher, and oysters we...
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ftarchimer:oai:archimer.ifremer.fr:2406 2023-05-15T15:58:51+02:00 Evidence for the involvement of pathogenic bacteria in summer mortalities of the Pacific oyster Crassostrea gigas Garnier, Matthieu Labreuche, Yannick Garcia, Celine Robert, Maeva Nicolas, Jean-louis 2007-02 application/pdf https://archimer.ifremer.fr/doc/2007/publication-2406.pdf https://doi.org/10.1007/s00248-006-9061-9 https://archimer.ifremer.fr/doc/00000/2406/ eng eng Springer https://archimer.ifremer.fr/doc/2007/publication-2406.pdf doi:10.1007/s00248-006-9061-9 https://archimer.ifremer.fr/doc/00000/2406/ 2007 Springer info:eu-repo/semantics/openAccess restricted use Microbial Ecology (0095-3628) (Springer), 2007-02 , Vol. 53 , N. 2 , P. 187-196 text Publication info:eu-repo/semantics/article 2007 ftarchimer https://doi.org/10.1007/s00248-006-9061-9 2021-09-23T20:14:37Z A study was conducted to investigate the involvement of bacteria in oyster mortalities during summer. Moribund and apparently healthy oysters were sampled during mortality events along the French coast and in rearing facilities, usually when temperature reached 19 degrees C or higher, and oysters were in the gonadal maturation phase. Hemolymph samples were aseptically withdrawn and submitted to bacteriological analysis. In healthy oysters, bacteria colonized hernolymph at low concentrations depending on the location. In most moribund oysters, bacteria were present in hemolymph and other tissues. These bacterial populations were more often diverse in oysters originating from the open sea than from facilities where animals were generally infected by a single type of bacterium. Only the dominant colonies were identified by phenotypic and genotypic characters (RFLP of GyrB gene and partial sequence of 16S rRNA gene). They belonged to a limited number of species including Vibrio aestuarianus, members of the V. splendidus group, V. natriegens, V. parahaemolyticus, and Pseudoalteromonas sp. The most frequently encountered species was V. aestuarlanus (56% of isolates), which was composed of several strains closely related by their 16S rRNA gene but diverse by their phenotypic characters. They appeared intimately linked to oysters. The species within the V. splendidus group were less prevalent (25% of isolates) and more taxonomically dispersed. A majority of the dominant strains of V. aestuarianus and V. splendidus group injected to oysters induced mortality, whereas others belonging to the same species, particularly those found in mixture, appeared innocuous. Article in Journal/Newspaper Crassostrea gigas Pacific oyster Archimer (Archive Institutionnelle de l'Ifremer - Institut français de recherche pour l'exploitation de la mer) Pacific Microbial Ecology 53 2 187 196 |
institution |
Open Polar |
collection |
Archimer (Archive Institutionnelle de l'Ifremer - Institut français de recherche pour l'exploitation de la mer) |
op_collection_id |
ftarchimer |
language |
English |
description |
A study was conducted to investigate the involvement of bacteria in oyster mortalities during summer. Moribund and apparently healthy oysters were sampled during mortality events along the French coast and in rearing facilities, usually when temperature reached 19 degrees C or higher, and oysters were in the gonadal maturation phase. Hemolymph samples were aseptically withdrawn and submitted to bacteriological analysis. In healthy oysters, bacteria colonized hernolymph at low concentrations depending on the location. In most moribund oysters, bacteria were present in hemolymph and other tissues. These bacterial populations were more often diverse in oysters originating from the open sea than from facilities where animals were generally infected by a single type of bacterium. Only the dominant colonies were identified by phenotypic and genotypic characters (RFLP of GyrB gene and partial sequence of 16S rRNA gene). They belonged to a limited number of species including Vibrio aestuarianus, members of the V. splendidus group, V. natriegens, V. parahaemolyticus, and Pseudoalteromonas sp. The most frequently encountered species was V. aestuarlanus (56% of isolates), which was composed of several strains closely related by their 16S rRNA gene but diverse by their phenotypic characters. They appeared intimately linked to oysters. The species within the V. splendidus group were less prevalent (25% of isolates) and more taxonomically dispersed. A majority of the dominant strains of V. aestuarianus and V. splendidus group injected to oysters induced mortality, whereas others belonging to the same species, particularly those found in mixture, appeared innocuous. |
format |
Article in Journal/Newspaper |
author |
Garnier, Matthieu Labreuche, Yannick Garcia, Celine Robert, Maeva Nicolas, Jean-louis |
spellingShingle |
Garnier, Matthieu Labreuche, Yannick Garcia, Celine Robert, Maeva Nicolas, Jean-louis Evidence for the involvement of pathogenic bacteria in summer mortalities of the Pacific oyster Crassostrea gigas |
author_facet |
Garnier, Matthieu Labreuche, Yannick Garcia, Celine Robert, Maeva Nicolas, Jean-louis |
author_sort |
Garnier, Matthieu |
title |
Evidence for the involvement of pathogenic bacteria in summer mortalities of the Pacific oyster Crassostrea gigas |
title_short |
Evidence for the involvement of pathogenic bacteria in summer mortalities of the Pacific oyster Crassostrea gigas |
title_full |
Evidence for the involvement of pathogenic bacteria in summer mortalities of the Pacific oyster Crassostrea gigas |
title_fullStr |
Evidence for the involvement of pathogenic bacteria in summer mortalities of the Pacific oyster Crassostrea gigas |
title_full_unstemmed |
Evidence for the involvement of pathogenic bacteria in summer mortalities of the Pacific oyster Crassostrea gigas |
title_sort |
evidence for the involvement of pathogenic bacteria in summer mortalities of the pacific oyster crassostrea gigas |
publisher |
Springer |
publishDate |
2007 |
url |
https://archimer.ifremer.fr/doc/2007/publication-2406.pdf https://doi.org/10.1007/s00248-006-9061-9 https://archimer.ifremer.fr/doc/00000/2406/ |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
Microbial Ecology (0095-3628) (Springer), 2007-02 , Vol. 53 , N. 2 , P. 187-196 |
op_relation |
https://archimer.ifremer.fr/doc/2007/publication-2406.pdf doi:10.1007/s00248-006-9061-9 https://archimer.ifremer.fr/doc/00000/2406/ |
op_rights |
2007 Springer info:eu-repo/semantics/openAccess restricted use |
op_doi |
https://doi.org/10.1007/s00248-006-9061-9 |
container_title |
Microbial Ecology |
container_volume |
53 |
container_issue |
2 |
container_start_page |
187 |
op_container_end_page |
196 |
_version_ |
1766394627203530752 |