First molecular evidence of cross-species induction of metalloprotease gene expression in Vibrio strains pathogenic for Pacific oyster Crassostrea gigas involving a quorum sensing system
The aim of that studywas to explore the hypothesis that a quorumsensingmechanismmodulatesmetalloprotease gene expression of two Vibrio species pathogenic for oyster, at intraspecific (Vibrio splendidus) and interspecific (V. splendidus affecting V. aestuarianus) levels. Metalloprotease activities (V...
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Online Access: | https://archimer.ifremer.fr/doc/00124/23539/21381.pdf https://doi.org/10.1016/j.aquaculture.2013.01.033 https://archimer.ifremer.fr/doc/00124/23539/ |
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ftarchimer:oai:archimer.ifremer.fr:23539 2023-05-15T15:58:01+02:00 First molecular evidence of cross-species induction of metalloprotease gene expression in Vibrio strains pathogenic for Pacific oyster Crassostrea gigas involving a quorum sensing system De Decker, Sophie Reynaud, Yann Saulnier, Denis 2013-05 application/pdf https://archimer.ifremer.fr/doc/00124/23539/21381.pdf https://doi.org/10.1016/j.aquaculture.2013.01.033 https://archimer.ifremer.fr/doc/00124/23539/ eng eng Elsevier Science Bv https://archimer.ifremer.fr/doc/00124/23539/21381.pdf doi:10.1016/j.aquaculture.2013.01.033 https://archimer.ifremer.fr/doc/00124/23539/ 2013 Elsevier B.V. All rights reserved info:eu-repo/semantics/openAccess restricted use Aquaculture (0044-8486) (Elsevier Science Bv), 2013-05 , Vol. 392 , P. 1-7 Quorum sensing Crassostrea gigas Vibrio Metalloprotease Gene expression text Publication info:eu-repo/semantics/article 2013 ftarchimer https://doi.org/10.1016/j.aquaculture.2013.01.033 2022-05-17T22:50:00Z The aim of that studywas to explore the hypothesis that a quorumsensingmechanismmodulatesmetalloprotease gene expression of two Vibrio species pathogenic for oyster, at intraspecific (Vibrio splendidus) and interspecific (V. splendidus affecting V. aestuarianus) levels. Metalloprotease activities (Vsm for V. splendidus LGP32 and Vam for V. aestuarianus 02/041) and growth curves obtained by real-time PCR assay revealed cell density-dependent metalloprotease inductions triggered between 12 h and 16 h of culture. A quorum sensing assay was then developed using a conditioned medium prepared from supernatant of a mutant strain of V. splendidus LGP32 unable to produce metalloprotease. Specific real-time qPCR assays targeting metalloprotease genes vsm and vam were performed. A relative increase in expressionwas observed for vsm and, particularly, vam in the presence of the conditioned medium, probably controlled by a quorumsensing system. The study revealed intraspecific and interspecific modulation of expression in metalloprotease genes vsm and vam in V. splendidus and V. aestuarianus. Our experiments showed for the first time that V. splendidus is able to produce an autoinducer-like substance that displays intra- and interspecific effects on the expression of two different metalloprotease genes: vsm and vam Article in Journal/Newspaper Crassostrea gigas Pacific oyster Archimer (Archive Institutionnelle de l'Ifremer - Institut français de recherche pour l'exploitation de la mer) Pacific Aquaculture 392-395 1 7 |
institution |
Open Polar |
collection |
Archimer (Archive Institutionnelle de l'Ifremer - Institut français de recherche pour l'exploitation de la mer) |
op_collection_id |
ftarchimer |
language |
English |
topic |
Quorum sensing Crassostrea gigas Vibrio Metalloprotease Gene expression |
spellingShingle |
Quorum sensing Crassostrea gigas Vibrio Metalloprotease Gene expression De Decker, Sophie Reynaud, Yann Saulnier, Denis First molecular evidence of cross-species induction of metalloprotease gene expression in Vibrio strains pathogenic for Pacific oyster Crassostrea gigas involving a quorum sensing system |
topic_facet |
Quorum sensing Crassostrea gigas Vibrio Metalloprotease Gene expression |
description |
The aim of that studywas to explore the hypothesis that a quorumsensingmechanismmodulatesmetalloprotease gene expression of two Vibrio species pathogenic for oyster, at intraspecific (Vibrio splendidus) and interspecific (V. splendidus affecting V. aestuarianus) levels. Metalloprotease activities (Vsm for V. splendidus LGP32 and Vam for V. aestuarianus 02/041) and growth curves obtained by real-time PCR assay revealed cell density-dependent metalloprotease inductions triggered between 12 h and 16 h of culture. A quorum sensing assay was then developed using a conditioned medium prepared from supernatant of a mutant strain of V. splendidus LGP32 unable to produce metalloprotease. Specific real-time qPCR assays targeting metalloprotease genes vsm and vam were performed. A relative increase in expressionwas observed for vsm and, particularly, vam in the presence of the conditioned medium, probably controlled by a quorumsensing system. The study revealed intraspecific and interspecific modulation of expression in metalloprotease genes vsm and vam in V. splendidus and V. aestuarianus. Our experiments showed for the first time that V. splendidus is able to produce an autoinducer-like substance that displays intra- and interspecific effects on the expression of two different metalloprotease genes: vsm and vam |
format |
Article in Journal/Newspaper |
author |
De Decker, Sophie Reynaud, Yann Saulnier, Denis |
author_facet |
De Decker, Sophie Reynaud, Yann Saulnier, Denis |
author_sort |
De Decker, Sophie |
title |
First molecular evidence of cross-species induction of metalloprotease gene expression in Vibrio strains pathogenic for Pacific oyster Crassostrea gigas involving a quorum sensing system |
title_short |
First molecular evidence of cross-species induction of metalloprotease gene expression in Vibrio strains pathogenic for Pacific oyster Crassostrea gigas involving a quorum sensing system |
title_full |
First molecular evidence of cross-species induction of metalloprotease gene expression in Vibrio strains pathogenic for Pacific oyster Crassostrea gigas involving a quorum sensing system |
title_fullStr |
First molecular evidence of cross-species induction of metalloprotease gene expression in Vibrio strains pathogenic for Pacific oyster Crassostrea gigas involving a quorum sensing system |
title_full_unstemmed |
First molecular evidence of cross-species induction of metalloprotease gene expression in Vibrio strains pathogenic for Pacific oyster Crassostrea gigas involving a quorum sensing system |
title_sort |
first molecular evidence of cross-species induction of metalloprotease gene expression in vibrio strains pathogenic for pacific oyster crassostrea gigas involving a quorum sensing system |
publisher |
Elsevier Science Bv |
publishDate |
2013 |
url |
https://archimer.ifremer.fr/doc/00124/23539/21381.pdf https://doi.org/10.1016/j.aquaculture.2013.01.033 https://archimer.ifremer.fr/doc/00124/23539/ |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
Aquaculture (0044-8486) (Elsevier Science Bv), 2013-05 , Vol. 392 , P. 1-7 |
op_relation |
https://archimer.ifremer.fr/doc/00124/23539/21381.pdf doi:10.1016/j.aquaculture.2013.01.033 https://archimer.ifremer.fr/doc/00124/23539/ |
op_rights |
2013 Elsevier B.V. All rights reserved info:eu-repo/semantics/openAccess restricted use |
op_doi |
https://doi.org/10.1016/j.aquaculture.2013.01.033 |
container_title |
Aquaculture |
container_volume |
392-395 |
container_start_page |
1 |
op_container_end_page |
7 |
_version_ |
1766393738830020608 |