Regulation of a truncated isoform of AMP-activated protein kinase alpha (AMPKalpha) in response to hypoxia in the muscle of Pacific oyster Crassostrea gigas
AMP-activated protein kinase α (AMPKα) is a key regulator of energy balance in many model species during hypoxia. In a marine bivalve, the Pacific oyster Crassostrea gigas, we analyzed the protein content of adductor muscle in response to hypoxia during 6 h. In both smooth and striated muscles, the...
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ftarchimer:oai:archimer.ifremer.fr:22998 2023-05-15T15:57:44+02:00 Regulation of a truncated isoform of AMP-activated protein kinase alpha (AMPKalpha) in response to hypoxia in the muscle of Pacific oyster Crassostrea gigas Guevelou, Eric Huvet, Arnaud Sussarellu, Rossana Milan, Massimo Guo, Ximing Li, Li Zhang, Guofan Quillien, Virgile Daniel, Jean-yves Quere, Claudie Boudry, Pierre Corporeau, Charlotte 2013-07 application/pdf https://archimer.ifremer.fr/doc/00119/22998/21397.pdf https://doi.org/10.1007/s00360-013-0743-6 https://archimer.ifremer.fr/doc/00119/22998/ eng eng Springer Heidelberg https://archimer.ifremer.fr/doc/00119/22998/21397.pdf doi:10.1007/s00360-013-0743-6 https://archimer.ifremer.fr/doc/00119/22998/ 2013, Springer-Verlag Berlin Heidelberg info:eu-repo/semantics/openAccess restricted use Journal Of Comparative Physiology B-biochemical Systemic And Environmental Physiology (0174-1578) (Springer Heidelberg), 2013-07 , Vol. 183 , N. 5 , P. 597-611 Marine bivalve Crassostrea gigas Hypoxia AMP-activated protein kinase Alternative splicing Adductor muscle text Publication info:eu-repo/semantics/article 2013 ftarchimer https://doi.org/10.1007/s00360-013-0743-6 2021-09-23T20:22:15Z AMP-activated protein kinase α (AMPKα) is a key regulator of energy balance in many model species during hypoxia. In a marine bivalve, the Pacific oyster Crassostrea gigas, we analyzed the protein content of adductor muscle in response to hypoxia during 6 h. In both smooth and striated muscles, the amount of full-length AMP-activated protein kinase α (AMPKα) remained unchanged during hypoxia. However, hypoxia induced a rapid and muscle-specific response concerning truncated isoforms of AMPKα. In the smooth muscle, a truncated isoform of AMPKα was increased from 1 to 6 h of hypoxia, and was linked with accumulation of AKT kinase, a key enzyme of the insulin signaling pathway which controls intracellular glucose metabolism. In this muscle, aerobic metabolism was maintained over the 6 h of hypoxia, as mitochondrial citrate synthase activity remained constant. In contrast, in striated muscle, hypoxia did not induce any significant modification of neither truncated AMPKα nor AKT protein content, and citrate synthase activity was altered after 6 h of hypoxia. Together, our results demonstrate that hypoxia response is specific to muscle type in Pacific oyster, and that truncated AMPKα and AKT proteins might be involved in maintaining aerobic metabolism in smooth muscle. Such regulation might occur in vivo during tidal intervals that cause up to 6 h of hypoxia. Article in Journal/Newspaper Crassostrea gigas Pacific oyster Archimer (Archive Institutionnelle de l'Ifremer - Institut français de recherche pour l'exploitation de la mer) Pacific Journal of Comparative Physiology B 183 5 597 611 |
institution |
Open Polar |
collection |
Archimer (Archive Institutionnelle de l'Ifremer - Institut français de recherche pour l'exploitation de la mer) |
op_collection_id |
ftarchimer |
language |
English |
topic |
Marine bivalve Crassostrea gigas Hypoxia AMP-activated protein kinase Alternative splicing Adductor muscle |
spellingShingle |
Marine bivalve Crassostrea gigas Hypoxia AMP-activated protein kinase Alternative splicing Adductor muscle Guevelou, Eric Huvet, Arnaud Sussarellu, Rossana Milan, Massimo Guo, Ximing Li, Li Zhang, Guofan Quillien, Virgile Daniel, Jean-yves Quere, Claudie Boudry, Pierre Corporeau, Charlotte Regulation of a truncated isoform of AMP-activated protein kinase alpha (AMPKalpha) in response to hypoxia in the muscle of Pacific oyster Crassostrea gigas |
topic_facet |
Marine bivalve Crassostrea gigas Hypoxia AMP-activated protein kinase Alternative splicing Adductor muscle |
description |
AMP-activated protein kinase α (AMPKα) is a key regulator of energy balance in many model species during hypoxia. In a marine bivalve, the Pacific oyster Crassostrea gigas, we analyzed the protein content of adductor muscle in response to hypoxia during 6 h. In both smooth and striated muscles, the amount of full-length AMP-activated protein kinase α (AMPKα) remained unchanged during hypoxia. However, hypoxia induced a rapid and muscle-specific response concerning truncated isoforms of AMPKα. In the smooth muscle, a truncated isoform of AMPKα was increased from 1 to 6 h of hypoxia, and was linked with accumulation of AKT kinase, a key enzyme of the insulin signaling pathway which controls intracellular glucose metabolism. In this muscle, aerobic metabolism was maintained over the 6 h of hypoxia, as mitochondrial citrate synthase activity remained constant. In contrast, in striated muscle, hypoxia did not induce any significant modification of neither truncated AMPKα nor AKT protein content, and citrate synthase activity was altered after 6 h of hypoxia. Together, our results demonstrate that hypoxia response is specific to muscle type in Pacific oyster, and that truncated AMPKα and AKT proteins might be involved in maintaining aerobic metabolism in smooth muscle. Such regulation might occur in vivo during tidal intervals that cause up to 6 h of hypoxia. |
format |
Article in Journal/Newspaper |
author |
Guevelou, Eric Huvet, Arnaud Sussarellu, Rossana Milan, Massimo Guo, Ximing Li, Li Zhang, Guofan Quillien, Virgile Daniel, Jean-yves Quere, Claudie Boudry, Pierre Corporeau, Charlotte |
author_facet |
Guevelou, Eric Huvet, Arnaud Sussarellu, Rossana Milan, Massimo Guo, Ximing Li, Li Zhang, Guofan Quillien, Virgile Daniel, Jean-yves Quere, Claudie Boudry, Pierre Corporeau, Charlotte |
author_sort |
Guevelou, Eric |
title |
Regulation of a truncated isoform of AMP-activated protein kinase alpha (AMPKalpha) in response to hypoxia in the muscle of Pacific oyster Crassostrea gigas |
title_short |
Regulation of a truncated isoform of AMP-activated protein kinase alpha (AMPKalpha) in response to hypoxia in the muscle of Pacific oyster Crassostrea gigas |
title_full |
Regulation of a truncated isoform of AMP-activated protein kinase alpha (AMPKalpha) in response to hypoxia in the muscle of Pacific oyster Crassostrea gigas |
title_fullStr |
Regulation of a truncated isoform of AMP-activated protein kinase alpha (AMPKalpha) in response to hypoxia in the muscle of Pacific oyster Crassostrea gigas |
title_full_unstemmed |
Regulation of a truncated isoform of AMP-activated protein kinase alpha (AMPKalpha) in response to hypoxia in the muscle of Pacific oyster Crassostrea gigas |
title_sort |
regulation of a truncated isoform of amp-activated protein kinase alpha (ampkalpha) in response to hypoxia in the muscle of pacific oyster crassostrea gigas |
publisher |
Springer Heidelberg |
publishDate |
2013 |
url |
https://archimer.ifremer.fr/doc/00119/22998/21397.pdf https://doi.org/10.1007/s00360-013-0743-6 https://archimer.ifremer.fr/doc/00119/22998/ |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
Journal Of Comparative Physiology B-biochemical Systemic And Environmental Physiology (0174-1578) (Springer Heidelberg), 2013-07 , Vol. 183 , N. 5 , P. 597-611 |
op_relation |
https://archimer.ifremer.fr/doc/00119/22998/21397.pdf doi:10.1007/s00360-013-0743-6 https://archimer.ifremer.fr/doc/00119/22998/ |
op_rights |
2013, Springer-Verlag Berlin Heidelberg info:eu-repo/semantics/openAccess restricted use |
op_doi |
https://doi.org/10.1007/s00360-013-0743-6 |
container_title |
Journal of Comparative Physiology B |
container_volume |
183 |
container_issue |
5 |
container_start_page |
597 |
op_container_end_page |
611 |
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1766393425996808192 |