Study of the antioxidant capacity in gills of the Pacific oyster Crassostrea gigas in link with its reproductive investment
Energy allocation principle is a core element of life-history theory in which "the cost of reproduction" corresponds to an acceleration of senescence caused by an increase in reproductive investment. In the "theory of aging", senescence is mainly due to the degradation of lipids,...
Published in: | Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology |
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Online Access: | https://archimer.ifremer.fr/doc/00116/22744/21494.pdf https://doi.org/10.1016/j.cbpc.2012.10.004 https://archimer.ifremer.fr/doc/00116/22744/ |
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ftarchimer:oai:archimer.ifremer.fr:22744 2023-05-15T15:57:42+02:00 Study of the antioxidant capacity in gills of the Pacific oyster Crassostrea gigas in link with its reproductive investment Beguel, Jean-philippe Huvet, Arnaud Quillien, Virgile Lambert, Christophe Fabioux, Caroline 2013-01 application/pdf https://archimer.ifremer.fr/doc/00116/22744/21494.pdf https://doi.org/10.1016/j.cbpc.2012.10.004 https://archimer.ifremer.fr/doc/00116/22744/ eng eng Elsevier Science Inc https://archimer.ifremer.fr/doc/00116/22744/21494.pdf doi:10.1016/j.cbpc.2012.10.004 https://archimer.ifremer.fr/doc/00116/22744/ 2012 Elsevier Inc. All rights reserved info:eu-repo/semantics/openAccess restricted use Comparative Biochemistry And Physiology C-toxicology & Pharmacology (1532-0456) (Elsevier Science Inc), 2013-01 , Vol. 157 , N. 1 , P. 63-71 Antioxidant capacity Crassostrea gigas Reproduction RNA interference ROS text Publication info:eu-repo/semantics/article 2013 ftarchimer https://doi.org/10.1016/j.cbpc.2012.10.004 2021-09-23T20:22:11Z Energy allocation principle is a core element of life-history theory in which "the cost of reproduction" corresponds to an acceleration of senescence caused by an increase in reproductive investment. In the "theory of aging", senescence is mainly due to the degradation of lipids, proteins and DNA by reactive oxygen species (ROS), by-products of oxidative metabolism. Some studies have shown that oxidative stress susceptibility could be a cost of reproduction. The present study investigates the effect of reproductive investment on antioxidant capacity in the gills of a species with a very high reproductive investment, the Pacific oyster Crassostrea gigas. We used RNA interference targeting the oyster vasa-like gene (Oyvlg) to produce oysters with contrasted reproductive investment. Antioxidant capacity was studied by measuring the mRNA levels of genes encoding major antioxidant enzymes, and the activity of these enzymes. The highest reproductive investment was associated with the highest transcript levels for glutathione peroxidase and extra-cellular and mitochondrial superoxide dismutase. In contrast, lipid peroxidation did not show any sign of oxidative damage whatever the reproductive investment. Up-regulation of certain genes encoding enzymes involved in the first step of ROS detoxification could therefore be a part of the organism's strategy for managing the pro-oxidant species produced by heavy reproductive investment. (C) 2012 Elsevier Inc. All rights reserved. Article in Journal/Newspaper Crassostrea gigas Pacific oyster Archimer (Archive Institutionnelle de l'Ifremer - Institut français de recherche pour l'exploitation de la mer) Pacific Vasa ENVELOPE(25.177,25.177,67.587,67.587) Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology 157 1 63 71 |
institution |
Open Polar |
collection |
Archimer (Archive Institutionnelle de l'Ifremer - Institut français de recherche pour l'exploitation de la mer) |
op_collection_id |
ftarchimer |
language |
English |
topic |
Antioxidant capacity Crassostrea gigas Reproduction RNA interference ROS |
spellingShingle |
Antioxidant capacity Crassostrea gigas Reproduction RNA interference ROS Beguel, Jean-philippe Huvet, Arnaud Quillien, Virgile Lambert, Christophe Fabioux, Caroline Study of the antioxidant capacity in gills of the Pacific oyster Crassostrea gigas in link with its reproductive investment |
topic_facet |
Antioxidant capacity Crassostrea gigas Reproduction RNA interference ROS |
description |
Energy allocation principle is a core element of life-history theory in which "the cost of reproduction" corresponds to an acceleration of senescence caused by an increase in reproductive investment. In the "theory of aging", senescence is mainly due to the degradation of lipids, proteins and DNA by reactive oxygen species (ROS), by-products of oxidative metabolism. Some studies have shown that oxidative stress susceptibility could be a cost of reproduction. The present study investigates the effect of reproductive investment on antioxidant capacity in the gills of a species with a very high reproductive investment, the Pacific oyster Crassostrea gigas. We used RNA interference targeting the oyster vasa-like gene (Oyvlg) to produce oysters with contrasted reproductive investment. Antioxidant capacity was studied by measuring the mRNA levels of genes encoding major antioxidant enzymes, and the activity of these enzymes. The highest reproductive investment was associated with the highest transcript levels for glutathione peroxidase and extra-cellular and mitochondrial superoxide dismutase. In contrast, lipid peroxidation did not show any sign of oxidative damage whatever the reproductive investment. Up-regulation of certain genes encoding enzymes involved in the first step of ROS detoxification could therefore be a part of the organism's strategy for managing the pro-oxidant species produced by heavy reproductive investment. (C) 2012 Elsevier Inc. All rights reserved. |
format |
Article in Journal/Newspaper |
author |
Beguel, Jean-philippe Huvet, Arnaud Quillien, Virgile Lambert, Christophe Fabioux, Caroline |
author_facet |
Beguel, Jean-philippe Huvet, Arnaud Quillien, Virgile Lambert, Christophe Fabioux, Caroline |
author_sort |
Beguel, Jean-philippe |
title |
Study of the antioxidant capacity in gills of the Pacific oyster Crassostrea gigas in link with its reproductive investment |
title_short |
Study of the antioxidant capacity in gills of the Pacific oyster Crassostrea gigas in link with its reproductive investment |
title_full |
Study of the antioxidant capacity in gills of the Pacific oyster Crassostrea gigas in link with its reproductive investment |
title_fullStr |
Study of the antioxidant capacity in gills of the Pacific oyster Crassostrea gigas in link with its reproductive investment |
title_full_unstemmed |
Study of the antioxidant capacity in gills of the Pacific oyster Crassostrea gigas in link with its reproductive investment |
title_sort |
study of the antioxidant capacity in gills of the pacific oyster crassostrea gigas in link with its reproductive investment |
publisher |
Elsevier Science Inc |
publishDate |
2013 |
url |
https://archimer.ifremer.fr/doc/00116/22744/21494.pdf https://doi.org/10.1016/j.cbpc.2012.10.004 https://archimer.ifremer.fr/doc/00116/22744/ |
long_lat |
ENVELOPE(25.177,25.177,67.587,67.587) |
geographic |
Pacific Vasa |
geographic_facet |
Pacific Vasa |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
Comparative Biochemistry And Physiology C-toxicology & Pharmacology (1532-0456) (Elsevier Science Inc), 2013-01 , Vol. 157 , N. 1 , P. 63-71 |
op_relation |
https://archimer.ifremer.fr/doc/00116/22744/21494.pdf doi:10.1016/j.cbpc.2012.10.004 https://archimer.ifremer.fr/doc/00116/22744/ |
op_rights |
2012 Elsevier Inc. All rights reserved info:eu-repo/semantics/openAccess restricted use |
op_doi |
https://doi.org/10.1016/j.cbpc.2012.10.004 |
container_title |
Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology |
container_volume |
157 |
container_issue |
1 |
container_start_page |
63 |
op_container_end_page |
71 |
_version_ |
1766393379105538048 |