Diversity of cultured photosynthetic flagellates in the northeast Pacific and Arctic Oceans in summer
During the MALINA cruise (summer 2009), an extensive effort was undertaken to isolate phytoplankton strains from the northeast (NE) Pacific Ocean, the Bering Strait, the Chukchi Sea, and the Beaufort Sea. In order to characterise the main photosynthetic microorganisms occurring in the Arctic during...
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Copernicus Gesellschaft Mbh
2012
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Online Access: | https://archimer.ifremer.fr/doc/00114/22491/20172.pdf https://archimer.ifremer.fr/doc/00114/22491/20173.pdf https://doi.org/10.5194/bg-9-4553-2012 https://archimer.ifremer.fr/doc/00114/22491/ |
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ftarchimer:oai:archimer.ifremer.fr:22491 2023-05-15T14:46:06+02:00 Diversity of cultured photosynthetic flagellates in the northeast Pacific and Arctic Oceans in summer Balzano, S. Gourvil, P. Siano, Raffaele Chanoine, M. Marie, D. Lessard, S. Sarno, D. Vaulot, Daniel 2012 application/pdf https://archimer.ifremer.fr/doc/00114/22491/20172.pdf https://archimer.ifremer.fr/doc/00114/22491/20173.pdf https://doi.org/10.5194/bg-9-4553-2012 https://archimer.ifremer.fr/doc/00114/22491/ eng eng Copernicus Gesellschaft Mbh https://archimer.ifremer.fr/doc/00114/22491/20172.pdf https://archimer.ifremer.fr/doc/00114/22491/20173.pdf doi:10.5194/bg-9-4553-2012 https://archimer.ifremer.fr/doc/00114/22491/ Author(s) 2012. CC Attribution 3.0 License. info:eu-repo/semantics/openAccess restricted use CC-BY Biogeosciences (1726-4170) (Copernicus Gesellschaft Mbh), 2012 , Vol. 9 , N. 11 , P. 4553-4571 text Publication info:eu-repo/semantics/article 2012 ftarchimer https://doi.org/10.5194/bg-9-4553-2012 2021-09-23T20:22:04Z During the MALINA cruise (summer 2009), an extensive effort was undertaken to isolate phytoplankton strains from the northeast (NE) Pacific Ocean, the Bering Strait, the Chukchi Sea, and the Beaufort Sea. In order to characterise the main photosynthetic microorganisms occurring in the Arctic during the summer season, strains were isolated by flow cytometry sorting (FCS) and single cell pipetting before or after phytoplankton enrichment of seawater samples. Strains were isolated both onboard and back in the laboratory and cultured at 4 °C under light/dark conditions. Overall, we isolated and characterised by light microscopy and 18 S rRNA gene sequencing 104 strains of photosynthetic flagellates which grouped into 21 genotypes (defined by 99.5% 18 S rRNA gene sequence similarity), mainly affiliated to Chlorophyta and Heterokontophyta. The taxon most frequently isolated was an Arctic ecotype of the green algal genus Micromonas (Arctic Micromonas), which was nearly the only phytoplankter recovered within the picoplankton (<2 μm) size range. Strains of Arctic Micromonas as well as other strains from the same class (Mamiellophyceae) were identified in further detail by sequencing the internal transcribed spacer (ITS) region of the rRNA operon. The MALINA Micromonas strains share identical 18 S rRNA and ITS sequences suggesting high genetic homogeneity within Arctic Micromonas. Three other Mamiellophyceae strains likely belong to a new genus. Other green algae from the genera Nephroselmis, Chlamydomonas, and Pyramimonas were also isolated, whereas Heterokontophyta included some unidentified Pelagophyceae, Dictyochophyceae (Pedinellales), and Chrysophyceae (Dinobryon faculiferum). Moreover, we isolated some Cryptophyceae (Rhodomonas sp.) as well as a few Prymnesiophyceae and dinoflagellates.We identified the dinoflagellateWoloszynskia cincta by scanning electron microscopy (SEM) and 28 S rRNA gene sequencing. Our morphological analyses show that this species possess the diagnostic features of the genus Biecheleria, and the 28 S rRNA gene topology corroborates this affiliation. We thus propose the transfer of W. cincta to the genus Biecheleria and its recombination as Biecheleria cincta. Article in Journal/Newspaper Arctic Beaufort Sea Bering Strait Chukchi Chukchi Sea Phytoplankton Archimer (Archive Institutionnelle de l'Ifremer - Institut français de recherche pour l'exploitation de la mer) Arctic Bering Strait Chukchi Sea Pacific Biogeosciences 9 11 4553 4571 |
institution |
Open Polar |
collection |
Archimer (Archive Institutionnelle de l'Ifremer - Institut français de recherche pour l'exploitation de la mer) |
op_collection_id |
ftarchimer |
language |
English |
description |
During the MALINA cruise (summer 2009), an extensive effort was undertaken to isolate phytoplankton strains from the northeast (NE) Pacific Ocean, the Bering Strait, the Chukchi Sea, and the Beaufort Sea. In order to characterise the main photosynthetic microorganisms occurring in the Arctic during the summer season, strains were isolated by flow cytometry sorting (FCS) and single cell pipetting before or after phytoplankton enrichment of seawater samples. Strains were isolated both onboard and back in the laboratory and cultured at 4 °C under light/dark conditions. Overall, we isolated and characterised by light microscopy and 18 S rRNA gene sequencing 104 strains of photosynthetic flagellates which grouped into 21 genotypes (defined by 99.5% 18 S rRNA gene sequence similarity), mainly affiliated to Chlorophyta and Heterokontophyta. The taxon most frequently isolated was an Arctic ecotype of the green algal genus Micromonas (Arctic Micromonas), which was nearly the only phytoplankter recovered within the picoplankton (<2 μm) size range. Strains of Arctic Micromonas as well as other strains from the same class (Mamiellophyceae) were identified in further detail by sequencing the internal transcribed spacer (ITS) region of the rRNA operon. The MALINA Micromonas strains share identical 18 S rRNA and ITS sequences suggesting high genetic homogeneity within Arctic Micromonas. Three other Mamiellophyceae strains likely belong to a new genus. Other green algae from the genera Nephroselmis, Chlamydomonas, and Pyramimonas were also isolated, whereas Heterokontophyta included some unidentified Pelagophyceae, Dictyochophyceae (Pedinellales), and Chrysophyceae (Dinobryon faculiferum). Moreover, we isolated some Cryptophyceae (Rhodomonas sp.) as well as a few Prymnesiophyceae and dinoflagellates.We identified the dinoflagellateWoloszynskia cincta by scanning electron microscopy (SEM) and 28 S rRNA gene sequencing. Our morphological analyses show that this species possess the diagnostic features of the genus Biecheleria, and the 28 S rRNA gene topology corroborates this affiliation. We thus propose the transfer of W. cincta to the genus Biecheleria and its recombination as Biecheleria cincta. |
format |
Article in Journal/Newspaper |
author |
Balzano, S. Gourvil, P. Siano, Raffaele Chanoine, M. Marie, D. Lessard, S. Sarno, D. Vaulot, Daniel |
spellingShingle |
Balzano, S. Gourvil, P. Siano, Raffaele Chanoine, M. Marie, D. Lessard, S. Sarno, D. Vaulot, Daniel Diversity of cultured photosynthetic flagellates in the northeast Pacific and Arctic Oceans in summer |
author_facet |
Balzano, S. Gourvil, P. Siano, Raffaele Chanoine, M. Marie, D. Lessard, S. Sarno, D. Vaulot, Daniel |
author_sort |
Balzano, S. |
title |
Diversity of cultured photosynthetic flagellates in the northeast Pacific and Arctic Oceans in summer |
title_short |
Diversity of cultured photosynthetic flagellates in the northeast Pacific and Arctic Oceans in summer |
title_full |
Diversity of cultured photosynthetic flagellates in the northeast Pacific and Arctic Oceans in summer |
title_fullStr |
Diversity of cultured photosynthetic flagellates in the northeast Pacific and Arctic Oceans in summer |
title_full_unstemmed |
Diversity of cultured photosynthetic flagellates in the northeast Pacific and Arctic Oceans in summer |
title_sort |
diversity of cultured photosynthetic flagellates in the northeast pacific and arctic oceans in summer |
publisher |
Copernicus Gesellschaft Mbh |
publishDate |
2012 |
url |
https://archimer.ifremer.fr/doc/00114/22491/20172.pdf https://archimer.ifremer.fr/doc/00114/22491/20173.pdf https://doi.org/10.5194/bg-9-4553-2012 https://archimer.ifremer.fr/doc/00114/22491/ |
geographic |
Arctic Bering Strait Chukchi Sea Pacific |
geographic_facet |
Arctic Bering Strait Chukchi Sea Pacific |
genre |
Arctic Beaufort Sea Bering Strait Chukchi Chukchi Sea Phytoplankton |
genre_facet |
Arctic Beaufort Sea Bering Strait Chukchi Chukchi Sea Phytoplankton |
op_source |
Biogeosciences (1726-4170) (Copernicus Gesellschaft Mbh), 2012 , Vol. 9 , N. 11 , P. 4553-4571 |
op_relation |
https://archimer.ifremer.fr/doc/00114/22491/20172.pdf https://archimer.ifremer.fr/doc/00114/22491/20173.pdf doi:10.5194/bg-9-4553-2012 https://archimer.ifremer.fr/doc/00114/22491/ |
op_rights |
Author(s) 2012. CC Attribution 3.0 License. info:eu-repo/semantics/openAccess restricted use |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.5194/bg-9-4553-2012 |
container_title |
Biogeosciences |
container_volume |
9 |
container_issue |
11 |
container_start_page |
4553 |
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4571 |
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