Cloning and expression analysis of allograft inflammatory factor type 1 in coelomocytes of antarctic sea urchin (Sterechinus neumayeri)
We have cloned and characterized for the first time an allograft inflammatory factor 1 (Sn-AIF-1) from the Antarctic sea urchin. We report the cloning of Sn-AIF-1 cDNA and the characterization of its expression in coelomocytes after a bacterial challenge. The cDNA Sn-AIF-1 has a size of 608 bp and e...
Published in: | Journal of Shellfish Research |
---|---|
Main Authors: | , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Natl Shellfisheries Assoc
2012
|
Subjects: | |
Online Access: | https://archimer.ifremer.fr/doc/00098/20955/18572.pdf https://doi.org/10.2983/035.031.0336 https://archimer.ifremer.fr/doc/00098/20955/ |
id |
ftarchimer:oai:archimer.ifremer.fr:20955 |
---|---|
record_format |
openpolar |
spelling |
ftarchimer:oai:archimer.ifremer.fr:20955 2023-05-15T13:47:21+02:00 Cloning and expression analysis of allograft inflammatory factor type 1 in coelomocytes of antarctic sea urchin (Sterechinus neumayeri) Ovando, Fernanda Gimpel, Carla Cardenas, Constanza Machado Cunha Da Silva, Jose Roberto De Lorgeril, Julien Gonzalez, Marcelo 2012-08 application/pdf https://archimer.ifremer.fr/doc/00098/20955/18572.pdf https://doi.org/10.2983/035.031.0336 https://archimer.ifremer.fr/doc/00098/20955/ eng eng Natl Shellfisheries Assoc https://archimer.ifremer.fr/doc/00098/20955/18572.pdf doi:10.2983/035.031.0336 https://archimer.ifremer.fr/doc/00098/20955/ 2012 BioOne All rights reserved, National Shellfisheries Association info:eu-repo/semantics/openAccess restricted use Journal Of Shellfish Research (0730-8000) (Natl Shellfisheries Assoc), 2012-08 , Vol. 31 , N. 3 , P. 875-883 Antarctica sea urchin Sterechinus neumayeri coelomocytes gene expression AIF-1 text Publication info:eu-repo/semantics/article 2012 ftarchimer https://doi.org/10.2983/035.031.0336 2021-09-23T20:21:17Z We have cloned and characterized for the first time an allograft inflammatory factor 1 (Sn-AIF-1) from the Antarctic sea urchin. We report the cloning of Sn-AIF-1 cDNA and the characterization of its expression in coelomocytes after a bacterial challenge. The cDNA Sn-AIF-1 has a size of 608 bp and encodes a polypeptide of 151 aa. The deduced amino acid sequence has a putative size of 17.430 Da, an isoelectric point of 4.92, and shows 2 elongation factor handlike motifs that normally bind calcium ions. BLAST analysis revealed close matches with other known AIF-1. The deduced amino acid sequence of Sn-AIF-1 showed high homology with AIF-1 in vertebrates such as fish, mice, and humans; and in the case of invertebrates, the major degree of identity (55%) was with a predicted sequence of the purple sea urchin AIF-1, and 52% corresponded to a sponge. Expression of Sn-AIF-1 mRNA was analyzed by qPCR. Sn-AIF-1 mRNA expression was measured from coelomocytes after a bacterial challenge using RT-PCR and revealed that the gene was upregulated after 24 h. Sn-AIF-1 could participate in the inflammatory response, particularly in the activation of coelomocytes and their survival. Article in Journal/Newspaper Antarc* Antarctic Antarctica Archimer (Archive Institutionnelle de l'Ifremer - Institut français de recherche pour l'exploitation de la mer) Antarctic The Antarctic Journal of Shellfish Research 31 3 875 883 |
institution |
Open Polar |
collection |
Archimer (Archive Institutionnelle de l'Ifremer - Institut français de recherche pour l'exploitation de la mer) |
op_collection_id |
ftarchimer |
language |
English |
topic |
Antarctica sea urchin Sterechinus neumayeri coelomocytes gene expression AIF-1 |
spellingShingle |
Antarctica sea urchin Sterechinus neumayeri coelomocytes gene expression AIF-1 Ovando, Fernanda Gimpel, Carla Cardenas, Constanza Machado Cunha Da Silva, Jose Roberto De Lorgeril, Julien Gonzalez, Marcelo Cloning and expression analysis of allograft inflammatory factor type 1 in coelomocytes of antarctic sea urchin (Sterechinus neumayeri) |
topic_facet |
Antarctica sea urchin Sterechinus neumayeri coelomocytes gene expression AIF-1 |
description |
We have cloned and characterized for the first time an allograft inflammatory factor 1 (Sn-AIF-1) from the Antarctic sea urchin. We report the cloning of Sn-AIF-1 cDNA and the characterization of its expression in coelomocytes after a bacterial challenge. The cDNA Sn-AIF-1 has a size of 608 bp and encodes a polypeptide of 151 aa. The deduced amino acid sequence has a putative size of 17.430 Da, an isoelectric point of 4.92, and shows 2 elongation factor handlike motifs that normally bind calcium ions. BLAST analysis revealed close matches with other known AIF-1. The deduced amino acid sequence of Sn-AIF-1 showed high homology with AIF-1 in vertebrates such as fish, mice, and humans; and in the case of invertebrates, the major degree of identity (55%) was with a predicted sequence of the purple sea urchin AIF-1, and 52% corresponded to a sponge. Expression of Sn-AIF-1 mRNA was analyzed by qPCR. Sn-AIF-1 mRNA expression was measured from coelomocytes after a bacterial challenge using RT-PCR and revealed that the gene was upregulated after 24 h. Sn-AIF-1 could participate in the inflammatory response, particularly in the activation of coelomocytes and their survival. |
format |
Article in Journal/Newspaper |
author |
Ovando, Fernanda Gimpel, Carla Cardenas, Constanza Machado Cunha Da Silva, Jose Roberto De Lorgeril, Julien Gonzalez, Marcelo |
author_facet |
Ovando, Fernanda Gimpel, Carla Cardenas, Constanza Machado Cunha Da Silva, Jose Roberto De Lorgeril, Julien Gonzalez, Marcelo |
author_sort |
Ovando, Fernanda |
title |
Cloning and expression analysis of allograft inflammatory factor type 1 in coelomocytes of antarctic sea urchin (Sterechinus neumayeri) |
title_short |
Cloning and expression analysis of allograft inflammatory factor type 1 in coelomocytes of antarctic sea urchin (Sterechinus neumayeri) |
title_full |
Cloning and expression analysis of allograft inflammatory factor type 1 in coelomocytes of antarctic sea urchin (Sterechinus neumayeri) |
title_fullStr |
Cloning and expression analysis of allograft inflammatory factor type 1 in coelomocytes of antarctic sea urchin (Sterechinus neumayeri) |
title_full_unstemmed |
Cloning and expression analysis of allograft inflammatory factor type 1 in coelomocytes of antarctic sea urchin (Sterechinus neumayeri) |
title_sort |
cloning and expression analysis of allograft inflammatory factor type 1 in coelomocytes of antarctic sea urchin (sterechinus neumayeri) |
publisher |
Natl Shellfisheries Assoc |
publishDate |
2012 |
url |
https://archimer.ifremer.fr/doc/00098/20955/18572.pdf https://doi.org/10.2983/035.031.0336 https://archimer.ifremer.fr/doc/00098/20955/ |
geographic |
Antarctic The Antarctic |
geographic_facet |
Antarctic The Antarctic |
genre |
Antarc* Antarctic Antarctica |
genre_facet |
Antarc* Antarctic Antarctica |
op_source |
Journal Of Shellfish Research (0730-8000) (Natl Shellfisheries Assoc), 2012-08 , Vol. 31 , N. 3 , P. 875-883 |
op_relation |
https://archimer.ifremer.fr/doc/00098/20955/18572.pdf doi:10.2983/035.031.0336 https://archimer.ifremer.fr/doc/00098/20955/ |
op_rights |
2012 BioOne All rights reserved, National Shellfisheries Association info:eu-repo/semantics/openAccess restricted use |
op_doi |
https://doi.org/10.2983/035.031.0336 |
container_title |
Journal of Shellfish Research |
container_volume |
31 |
container_issue |
3 |
container_start_page |
875 |
op_container_end_page |
883 |
_version_ |
1766246963457556480 |