First evidence of the activation of Cg-timp, an immune response component of pacific oysters, through a damage-associated molecular pattern pathway

• Published in: Developmental & Comparative Immunology
• Main Authors: Montagnani, Caroline, Avarre, Jean-christophe, De Lorgeril, Julien, Quiquand, M, Boulo, Viviane, Escoubas, Jean-michel
• Format: Article in Journal/Newspaper
• Language: English
• Published: Elsevier 2007
• Subjects: Damage associated molecular pattern; Tissue inhibitor of metalloproteinase; Metalloproteinase; Mollusk immunity; Crassostrea gigas; Oyster; Pacific
• Online Access: https://archimer.ifremer.fr/doc/2007/publication-2094.pdf; https://doi.org/10.1016/j.dci.2006.04.002; https://archimer.ifremer.fr/doc/00000/2094/

In a previous work, we characterized a Crassostrea gigas cPNA (Cg-timp) encoding a protein which presents all the features of vertebrate tissue inhibitor of metalloproteinase (TIMP). The expression pattern of this gene led us to propose that Cg-timp is an important factor in oyster wound heating and defense mechanisms. Here we describe the analysis of Cg-timp expression in oysters challenged by live or dead bacteria as well as by bacterial secretory/excretory products and metalloproteinase. Surprisingly, bacterial secretory/excretory products activate Cg-timp gene expression whereas heat-inactivated ones do not. To address the question of the signal transduction pathway involved in Cg-timp gene activation, we isolated and sequenced Cg-timp promoter and upstream region. A 1-kb genomic DNA fragment flanking the 5'-end of the gene contains several regulatory elements and notably three NF-kappa B binding sites. The potential involvement of these motifs in Cg-timp gene regulation is discussed. (c) 2006 Elsevier Ltd. All rights reserved.