Proteomic identification of quality factors for oocytes in the Pacific oyster Crassostrea gigas
We used a 2-DE proteomic approach to identify abundant proteins linked to oocyte quality in the Pacific oyster Crassostrea gigas, an economically important bivalve. Oocyte quality of 14 females was estimated by recording fertilisation and early developmental success until D-larval stage under contro...
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Online Access: | https://archimer.ifremer.fr/doc/00098/20925/18534.pdf https://doi.org/10.1016/j.jprot.2012.07.040 https://archimer.ifremer.fr/doc/00098/20925/ |
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ftarchimer:oai:archimer.ifremer.fr:20925 2023-05-15T15:57:37+02:00 Proteomic identification of quality factors for oocytes in the Pacific oyster Crassostrea gigas Corporeau, Charlotte Vanderplancke, Gwenaelle Boulais, Myrina Suquet, Marc Quere, Claudie Boudry, Pierre Huvet, Arnaud Madec, Stephanie 2012-10 application/pdf https://archimer.ifremer.fr/doc/00098/20925/18534.pdf https://doi.org/10.1016/j.jprot.2012.07.040 https://archimer.ifremer.fr/doc/00098/20925/ eng eng Elsevier Science Bv info:eu-repo/grantAgreement/EC/FP7/245119/EU//REPROSEED https://archimer.ifremer.fr/doc/00098/20925/18534.pdf doi:10.1016/j.jprot.2012.07.040 https://archimer.ifremer.fr/doc/00098/20925/ 2012 Elsevier B.V. All rights reserved. info:eu-repo/semantics/openAccess restricted use Journal Of Proteomics (1874-3919) (Elsevier Science Bv), 2012-10 , Vol. 75 , N. 18 , P. 5554-5563 Marine bivalves Crassostrea gigas Oocyte quality Proteomics text Publication info:eu-repo/semantics/article 2012 ftarchimer https://doi.org/10.1016/j.jprot.2012.07.040 2021-09-23T20:21:17Z We used a 2-DE proteomic approach to identify abundant proteins linked to oocyte quality in the Pacific oyster Crassostrea gigas, an economically important bivalve. Oocyte quality of 14 females was estimated by recording fertilisation and early developmental success until D-larval stage under controlled conditions. Proteins that were differentially expressed between females showing high or low oocyte quality were identified by nano-liquid chromatography tandem mass spectrometry. Twelve up-accumulated spots associated with low quality oocytes revealed 10 distinct proteins, including vitellogenin — breakdown products and metabolic enzymes. Eight up-accumulated spots from high quality oocytes revealed 6 distinct proteins, including chaperone molecules and cell-cycle control proteins. This is the first proteomic study dedicated to oocytes in C. gigas. Our results improve current knowledge about protein factors associated with oocyte quality in this species, and our understanding of the proteomic processes involved in their developmental competence. Highlights ► 14 Pacific oyster females were fertilised in vitro by a common pool of spermatozoa. ► Individual variability of D-larva yields was due to the intrinsic quality of oocytes. ► Two-dimensional proteomics identified protein factors for oocyte quality. Article in Journal/Newspaper Crassostrea gigas Pacific oyster Archimer (Archive Institutionnelle de l'Ifremer - Institut français de recherche pour l'exploitation de la mer) Pacific Journal of Proteomics 75 18 5554 5563 |
institution |
Open Polar |
collection |
Archimer (Archive Institutionnelle de l'Ifremer - Institut français de recherche pour l'exploitation de la mer) |
op_collection_id |
ftarchimer |
language |
English |
topic |
Marine bivalves Crassostrea gigas Oocyte quality Proteomics |
spellingShingle |
Marine bivalves Crassostrea gigas Oocyte quality Proteomics Corporeau, Charlotte Vanderplancke, Gwenaelle Boulais, Myrina Suquet, Marc Quere, Claudie Boudry, Pierre Huvet, Arnaud Madec, Stephanie Proteomic identification of quality factors for oocytes in the Pacific oyster Crassostrea gigas |
topic_facet |
Marine bivalves Crassostrea gigas Oocyte quality Proteomics |
description |
We used a 2-DE proteomic approach to identify abundant proteins linked to oocyte quality in the Pacific oyster Crassostrea gigas, an economically important bivalve. Oocyte quality of 14 females was estimated by recording fertilisation and early developmental success until D-larval stage under controlled conditions. Proteins that were differentially expressed between females showing high or low oocyte quality were identified by nano-liquid chromatography tandem mass spectrometry. Twelve up-accumulated spots associated with low quality oocytes revealed 10 distinct proteins, including vitellogenin — breakdown products and metabolic enzymes. Eight up-accumulated spots from high quality oocytes revealed 6 distinct proteins, including chaperone molecules and cell-cycle control proteins. This is the first proteomic study dedicated to oocytes in C. gigas. Our results improve current knowledge about protein factors associated with oocyte quality in this species, and our understanding of the proteomic processes involved in their developmental competence. Highlights ► 14 Pacific oyster females were fertilised in vitro by a common pool of spermatozoa. ► Individual variability of D-larva yields was due to the intrinsic quality of oocytes. ► Two-dimensional proteomics identified protein factors for oocyte quality. |
format |
Article in Journal/Newspaper |
author |
Corporeau, Charlotte Vanderplancke, Gwenaelle Boulais, Myrina Suquet, Marc Quere, Claudie Boudry, Pierre Huvet, Arnaud Madec, Stephanie |
author_facet |
Corporeau, Charlotte Vanderplancke, Gwenaelle Boulais, Myrina Suquet, Marc Quere, Claudie Boudry, Pierre Huvet, Arnaud Madec, Stephanie |
author_sort |
Corporeau, Charlotte |
title |
Proteomic identification of quality factors for oocytes in the Pacific oyster Crassostrea gigas |
title_short |
Proteomic identification of quality factors for oocytes in the Pacific oyster Crassostrea gigas |
title_full |
Proteomic identification of quality factors for oocytes in the Pacific oyster Crassostrea gigas |
title_fullStr |
Proteomic identification of quality factors for oocytes in the Pacific oyster Crassostrea gigas |
title_full_unstemmed |
Proteomic identification of quality factors for oocytes in the Pacific oyster Crassostrea gigas |
title_sort |
proteomic identification of quality factors for oocytes in the pacific oyster crassostrea gigas |
publisher |
Elsevier Science Bv |
publishDate |
2012 |
url |
https://archimer.ifremer.fr/doc/00098/20925/18534.pdf https://doi.org/10.1016/j.jprot.2012.07.040 https://archimer.ifremer.fr/doc/00098/20925/ |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
Journal Of Proteomics (1874-3919) (Elsevier Science Bv), 2012-10 , Vol. 75 , N. 18 , P. 5554-5563 |
op_relation |
info:eu-repo/grantAgreement/EC/FP7/245119/EU//REPROSEED https://archimer.ifremer.fr/doc/00098/20925/18534.pdf doi:10.1016/j.jprot.2012.07.040 https://archimer.ifremer.fr/doc/00098/20925/ |
op_rights |
2012 Elsevier B.V. All rights reserved. info:eu-repo/semantics/openAccess restricted use |
op_doi |
https://doi.org/10.1016/j.jprot.2012.07.040 |
container_title |
Journal of Proteomics |
container_volume |
75 |
container_issue |
18 |
container_start_page |
5554 |
op_container_end_page |
5563 |
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1766393283495329792 |