Starch supplementation modulates amylase enzymatic properties and amylase B mRNA level in the digestive gland of the Pacific oyster Crassostrea gigas
In the oyster Crassostrea gigas consumption-related traits, amylase properties and growth were found to be linked through genotypes that differed for polymorphism in the two amylase genes AMYA and AMYB. Modulation of AMYA mRNA level had already been observed in response to food availability, whereas...
Published in: | Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology |
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Online Access: | https://archimer.ifremer.fr/doc/00087/19869/17521.pdf https://doi.org/10.1016/j.cbpb.2012.05.003 https://archimer.ifremer.fr/doc/00087/19869/ |
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ftarchimer:oai:archimer.ifremer.fr:19869 2023-05-15T15:57:39+02:00 Starch supplementation modulates amylase enzymatic properties and amylase B mRNA level in the digestive gland of the Pacific oyster Crassostrea gigas Huvet, Arnaud Jeffroy, Fanny Daniel, Jean-yves Quere, Claudie Le Souchu, Pierrick Van Wormhoudt, A. Boudry, Pierre Moal, Jeanne Samain, Jean-francois 2012-09 application/pdf https://archimer.ifremer.fr/doc/00087/19869/17521.pdf https://doi.org/10.1016/j.cbpb.2012.05.003 https://archimer.ifremer.fr/doc/00087/19869/ eng eng Elsevier Science Inc https://archimer.ifremer.fr/doc/00087/19869/17521.pdf doi:10.1016/j.cbpb.2012.05.003 https://archimer.ifremer.fr/doc/00087/19869/ 2012 Elsevier Inc. All rights reserved. info:eu-repo/semantics/openAccess restricted use Comparative Biochemistry And Physiology B-biochemistry & Molecular Biology (1096-4959) (Elsevier Science Inc), 2012-09 , Vol. 163 , N. 1 , P. 96-100 alpha-amylase Crassostrea gigas digestive enzyme Michaelis-Menten constant regulation text Publication info:eu-repo/semantics/article 2012 ftarchimer https://doi.org/10.1016/j.cbpb.2012.05.003 2021-09-23T20:21:00Z In the oyster Crassostrea gigas consumption-related traits, amylase properties and growth were found to be linked through genotypes that differed for polymorphism in the two amylase genes AMYA and AMYB. Modulation of AMYA mRNA level had already been observed in response to food availability, whereas the functional role of AMYB was still unknown. To improve knowledge about the regulation of amylase expression in C. gigas and the respective roles of the two genes, we made an assay of amylase expression at mRNA and enzymatic levels in the digestive gland of oysters that had received dietary supplements of starch. After 18 days, a significant increase of translatable mRNA for AMYB was observed, with a correlated increase in Michaelis-Menten constant Km values and a decrease in total amylase activity. This modulation is the first evidence of observable functioning of AMYB in digestive processes. Amylase B is suggested to display a higher Km than amylase A, offering a means of adapting to high substrate concentrations. The highest starch supplement level (10 mg L− 1) induced alteration in oyster physiology. The 1 mg L− 1 treatment should be tested as a practical food supplement that could lead to growth benefits for oysters. Article in Journal/Newspaper Crassostrea gigas Pacific oyster Archimer (Archive Institutionnelle de l'Ifremer - Institut français de recherche pour l'exploitation de la mer) Pacific Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology 163 1 96 100 |
institution |
Open Polar |
collection |
Archimer (Archive Institutionnelle de l'Ifremer - Institut français de recherche pour l'exploitation de la mer) |
op_collection_id |
ftarchimer |
language |
English |
topic |
alpha-amylase Crassostrea gigas digestive enzyme Michaelis-Menten constant regulation |
spellingShingle |
alpha-amylase Crassostrea gigas digestive enzyme Michaelis-Menten constant regulation Huvet, Arnaud Jeffroy, Fanny Daniel, Jean-yves Quere, Claudie Le Souchu, Pierrick Van Wormhoudt, A. Boudry, Pierre Moal, Jeanne Samain, Jean-francois Starch supplementation modulates amylase enzymatic properties and amylase B mRNA level in the digestive gland of the Pacific oyster Crassostrea gigas |
topic_facet |
alpha-amylase Crassostrea gigas digestive enzyme Michaelis-Menten constant regulation |
description |
In the oyster Crassostrea gigas consumption-related traits, amylase properties and growth were found to be linked through genotypes that differed for polymorphism in the two amylase genes AMYA and AMYB. Modulation of AMYA mRNA level had already been observed in response to food availability, whereas the functional role of AMYB was still unknown. To improve knowledge about the regulation of amylase expression in C. gigas and the respective roles of the two genes, we made an assay of amylase expression at mRNA and enzymatic levels in the digestive gland of oysters that had received dietary supplements of starch. After 18 days, a significant increase of translatable mRNA for AMYB was observed, with a correlated increase in Michaelis-Menten constant Km values and a decrease in total amylase activity. This modulation is the first evidence of observable functioning of AMYB in digestive processes. Amylase B is suggested to display a higher Km than amylase A, offering a means of adapting to high substrate concentrations. The highest starch supplement level (10 mg L− 1) induced alteration in oyster physiology. The 1 mg L− 1 treatment should be tested as a practical food supplement that could lead to growth benefits for oysters. |
format |
Article in Journal/Newspaper |
author |
Huvet, Arnaud Jeffroy, Fanny Daniel, Jean-yves Quere, Claudie Le Souchu, Pierrick Van Wormhoudt, A. Boudry, Pierre Moal, Jeanne Samain, Jean-francois |
author_facet |
Huvet, Arnaud Jeffroy, Fanny Daniel, Jean-yves Quere, Claudie Le Souchu, Pierrick Van Wormhoudt, A. Boudry, Pierre Moal, Jeanne Samain, Jean-francois |
author_sort |
Huvet, Arnaud |
title |
Starch supplementation modulates amylase enzymatic properties and amylase B mRNA level in the digestive gland of the Pacific oyster Crassostrea gigas |
title_short |
Starch supplementation modulates amylase enzymatic properties and amylase B mRNA level in the digestive gland of the Pacific oyster Crassostrea gigas |
title_full |
Starch supplementation modulates amylase enzymatic properties and amylase B mRNA level in the digestive gland of the Pacific oyster Crassostrea gigas |
title_fullStr |
Starch supplementation modulates amylase enzymatic properties and amylase B mRNA level in the digestive gland of the Pacific oyster Crassostrea gigas |
title_full_unstemmed |
Starch supplementation modulates amylase enzymatic properties and amylase B mRNA level in the digestive gland of the Pacific oyster Crassostrea gigas |
title_sort |
starch supplementation modulates amylase enzymatic properties and amylase b mrna level in the digestive gland of the pacific oyster crassostrea gigas |
publisher |
Elsevier Science Inc |
publishDate |
2012 |
url |
https://archimer.ifremer.fr/doc/00087/19869/17521.pdf https://doi.org/10.1016/j.cbpb.2012.05.003 https://archimer.ifremer.fr/doc/00087/19869/ |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
Comparative Biochemistry And Physiology B-biochemistry & Molecular Biology (1096-4959) (Elsevier Science Inc), 2012-09 , Vol. 163 , N. 1 , P. 96-100 |
op_relation |
https://archimer.ifremer.fr/doc/00087/19869/17521.pdf doi:10.1016/j.cbpb.2012.05.003 https://archimer.ifremer.fr/doc/00087/19869/ |
op_rights |
2012 Elsevier Inc. All rights reserved. info:eu-repo/semantics/openAccess restricted use |
op_doi |
https://doi.org/10.1016/j.cbpb.2012.05.003 |
container_title |
Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology |
container_volume |
163 |
container_issue |
1 |
container_start_page |
96 |
op_container_end_page |
100 |
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1766393329565564928 |