In Vivo RNA Interference of a Gonad-Specific Transforming Growth Factor-beta in the Pacific Oyster Crassostrea gigas
We investigated the role of oyster gonadal TGF beta (og-TGF beta) in the reproduction of Crassostrea gigas, using an in vivo RNA interference approach. We designed double-stranded RNA targeting og-TGF beta, which is specifically expressed in the somatic cells surrounding germ cells in the gonad of b...
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ftarchimer:oai:archimer.ifremer.fr:19794 2023-05-15T15:57:39+02:00 In Vivo RNA Interference of a Gonad-Specific Transforming Growth Factor-beta in the Pacific Oyster Crassostrea gigas Huvet, Arnaud Fleury, Elodie Corporeau, Charlotte Quillien, Virgile Daniel, Jean-yves Riviere, Guillaume Boudry, Pierre Fabioux, Caroline 2012-08 application/pdf https://archimer.ifremer.fr/doc/00087/19794/17486.pdf https://doi.org/10.1007/s10126-011-9421-4 https://archimer.ifremer.fr/doc/00087/19794/ eng eng Springer https://archimer.ifremer.fr/doc/00087/19794/17486.pdf doi:10.1007/s10126-011-9421-4 https://archimer.ifremer.fr/doc/00087/19794/ Springer Science+Business Media, LLC 2011 info:eu-repo/semantics/openAccess restricted use Marine Biotechnology (1436-2228) (Springer), 2012-08 , Vol. 14 , N. 4 , P. 402-410 Marine bivalve Crassostrea gigas Reproduction RNA interference Transforming growth factor-beta text Publication info:eu-repo/semantics/article 2012 ftarchimer https://doi.org/10.1007/s10126-011-9421-4 2021-09-23T20:21:00Z We investigated the role of oyster gonadal TGF beta (og-TGF beta) in the reproduction of Crassostrea gigas, using an in vivo RNA interference approach. We designed double-stranded RNA targeting og-TGF beta, which is specifically expressed in the somatic cells surrounding germ cells in the gonad of both male and female oysters. In vivo injection of this og-TGF beta dsRNA into the gonad led to knock-down phenotypes for both sexes, with significant reduction (77.52% relative to controls) of the gonad area, lowered reproductive effort and germ cell under-proliferation. Interestingly, half of the injected females halted their vitellogenesis, since we were only able to observe pre-vitellogenic oocytes. In addition, apoptotic germ cells and haemocytes infiltrated into the gonad, likely as part of the active resorption of degenerating germ cells. Conversely, males showed a normal phenotype at the cellular level, with spermatids and spermatozoids observed in the gonads of control and injected males. As a result, og-TGF beta appears to play an essential role in C. gigas germ cell development by functioning as an activator of germ cell proliferation in both male and female oysters and vitellogenesis in females. Article in Journal/Newspaper Crassostrea gigas Pacific oyster Archimer (Archive Institutionnelle de l'Ifremer - Institut français de recherche pour l'exploitation de la mer) Pacific Marine Biotechnology 14 4 402 410 |
institution |
Open Polar |
collection |
Archimer (Archive Institutionnelle de l'Ifremer - Institut français de recherche pour l'exploitation de la mer) |
op_collection_id |
ftarchimer |
language |
English |
topic |
Marine bivalve Crassostrea gigas Reproduction RNA interference Transforming growth factor-beta |
spellingShingle |
Marine bivalve Crassostrea gigas Reproduction RNA interference Transforming growth factor-beta Huvet, Arnaud Fleury, Elodie Corporeau, Charlotte Quillien, Virgile Daniel, Jean-yves Riviere, Guillaume Boudry, Pierre Fabioux, Caroline In Vivo RNA Interference of a Gonad-Specific Transforming Growth Factor-beta in the Pacific Oyster Crassostrea gigas |
topic_facet |
Marine bivalve Crassostrea gigas Reproduction RNA interference Transforming growth factor-beta |
description |
We investigated the role of oyster gonadal TGF beta (og-TGF beta) in the reproduction of Crassostrea gigas, using an in vivo RNA interference approach. We designed double-stranded RNA targeting og-TGF beta, which is specifically expressed in the somatic cells surrounding germ cells in the gonad of both male and female oysters. In vivo injection of this og-TGF beta dsRNA into the gonad led to knock-down phenotypes for both sexes, with significant reduction (77.52% relative to controls) of the gonad area, lowered reproductive effort and germ cell under-proliferation. Interestingly, half of the injected females halted their vitellogenesis, since we were only able to observe pre-vitellogenic oocytes. In addition, apoptotic germ cells and haemocytes infiltrated into the gonad, likely as part of the active resorption of degenerating germ cells. Conversely, males showed a normal phenotype at the cellular level, with spermatids and spermatozoids observed in the gonads of control and injected males. As a result, og-TGF beta appears to play an essential role in C. gigas germ cell development by functioning as an activator of germ cell proliferation in both male and female oysters and vitellogenesis in females. |
format |
Article in Journal/Newspaper |
author |
Huvet, Arnaud Fleury, Elodie Corporeau, Charlotte Quillien, Virgile Daniel, Jean-yves Riviere, Guillaume Boudry, Pierre Fabioux, Caroline |
author_facet |
Huvet, Arnaud Fleury, Elodie Corporeau, Charlotte Quillien, Virgile Daniel, Jean-yves Riviere, Guillaume Boudry, Pierre Fabioux, Caroline |
author_sort |
Huvet, Arnaud |
title |
In Vivo RNA Interference of a Gonad-Specific Transforming Growth Factor-beta in the Pacific Oyster Crassostrea gigas |
title_short |
In Vivo RNA Interference of a Gonad-Specific Transforming Growth Factor-beta in the Pacific Oyster Crassostrea gigas |
title_full |
In Vivo RNA Interference of a Gonad-Specific Transforming Growth Factor-beta in the Pacific Oyster Crassostrea gigas |
title_fullStr |
In Vivo RNA Interference of a Gonad-Specific Transforming Growth Factor-beta in the Pacific Oyster Crassostrea gigas |
title_full_unstemmed |
In Vivo RNA Interference of a Gonad-Specific Transforming Growth Factor-beta in the Pacific Oyster Crassostrea gigas |
title_sort |
in vivo rna interference of a gonad-specific transforming growth factor-beta in the pacific oyster crassostrea gigas |
publisher |
Springer |
publishDate |
2012 |
url |
https://archimer.ifremer.fr/doc/00087/19794/17486.pdf https://doi.org/10.1007/s10126-011-9421-4 https://archimer.ifremer.fr/doc/00087/19794/ |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
Marine Biotechnology (1436-2228) (Springer), 2012-08 , Vol. 14 , N. 4 , P. 402-410 |
op_relation |
https://archimer.ifremer.fr/doc/00087/19794/17486.pdf doi:10.1007/s10126-011-9421-4 https://archimer.ifremer.fr/doc/00087/19794/ |
op_rights |
Springer Science+Business Media, LLC 2011 info:eu-repo/semantics/openAccess restricted use |
op_doi |
https://doi.org/10.1007/s10126-011-9421-4 |
container_title |
Marine Biotechnology |
container_volume |
14 |
container_issue |
4 |
container_start_page |
402 |
op_container_end_page |
410 |
_version_ |
1766393329381015552 |