Characterization of GnRH-related peptides from the Pacific oyster Crassostrea gigas
Gonadotropin-releasing hormone (GnRH), a key neuropeptide regulating reproduction in vertebrates has now been characterized in a number of non-vertebrate species. Despite the demonstration of its ancestral origin, the structure and the function of this family of peptides remain poorly known in speci...
Published in: | Peptides |
---|---|
Main Authors: | , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Elsevier Science Inc
2012
|
Subjects: | |
Online Access: | https://archimer.ifremer.fr/doc/00079/19003/17477.pdf https://doi.org/10.1016/j.peptides.2012.01.017 https://archimer.ifremer.fr/doc/00079/19003/ |
id |
ftarchimer:oai:archimer.ifremer.fr:19003 |
---|---|
record_format |
openpolar |
spelling |
ftarchimer:oai:archimer.ifremer.fr:19003 2023-05-15T15:58:01+02:00 Characterization of GnRH-related peptides from the Pacific oyster Crassostrea gigas Bigot, Laetitia Zatylny-gaudin, Celine Rodet, Franck Bernay, Benoit Boudry, Pierre Favrel, Pascal 2012-04 application/pdf https://archimer.ifremer.fr/doc/00079/19003/17477.pdf https://doi.org/10.1016/j.peptides.2012.01.017 https://archimer.ifremer.fr/doc/00079/19003/ eng eng Elsevier Science Inc https://archimer.ifremer.fr/doc/00079/19003/17477.pdf doi:10.1016/j.peptides.2012.01.017 https://archimer.ifremer.fr/doc/00079/19003/ 2012 Elsevier Inc. All rights reserved. info:eu-repo/semantics/openAccess restricted use Peptides (0196-9781) (Elsevier Science Inc), 2012-04 , Vol. 34 , N. 2 , P. 303-310 Mollusk Bivalve Oyster Neuroendocrinology Reproduction GnRH text Publication info:eu-repo/semantics/article 2012 ftarchimer https://doi.org/10.1016/j.peptides.2012.01.017 2021-09-23T20:20:42Z Gonadotropin-releasing hormone (GnRH), a key neuropeptide regulating reproduction in vertebrates has now been characterized in a number of non-vertebrate species. Despite the demonstration of its ancestral origin, the structure and the function of this family of peptides remain poorly known in species as distant as lophotrochozoans. In this study, two GnRH-related peptides (Cg-GnRH-a and CgGnRH-G) were characterized by mass spectrometry from extracts of the visceral ganglia of the Pacific oyster Crassostrea gigas. These peptides showed a high degree of sequence identity with GnRHs of other mollusks and annelids and to a lesser extent with those of vertebrates or with AKH and corazonins of insects. Both the mature peptides and the transcript encoding the precursor protein were exclusively expressed in the visceral ganglia. Significant differences in transcriptional activity of Cg-GnRH encoding gene were recorded in the ganglia along the reproductive cycle and according to trophic conditions with a higher level in fed animals compared to starved animals. This suggests the involvement of Cg-GnRHs as synchronizers of nutritional status with energy requirements during reproduction in oyster. Evidence for a role of Cg-GnRHs as neuroregulators and as neuroendocrine factors in bivalve is discussed. (C) 2012 Elsevier Inc. All rights reserved. Article in Journal/Newspaper Crassostrea gigas Pacific oyster Archimer (Archive Institutionnelle de l'Ifremer - Institut français de recherche pour l'exploitation de la mer) Pacific Peptides 34 2 303 310 |
institution |
Open Polar |
collection |
Archimer (Archive Institutionnelle de l'Ifremer - Institut français de recherche pour l'exploitation de la mer) |
op_collection_id |
ftarchimer |
language |
English |
topic |
Mollusk Bivalve Oyster Neuroendocrinology Reproduction GnRH |
spellingShingle |
Mollusk Bivalve Oyster Neuroendocrinology Reproduction GnRH Bigot, Laetitia Zatylny-gaudin, Celine Rodet, Franck Bernay, Benoit Boudry, Pierre Favrel, Pascal Characterization of GnRH-related peptides from the Pacific oyster Crassostrea gigas |
topic_facet |
Mollusk Bivalve Oyster Neuroendocrinology Reproduction GnRH |
description |
Gonadotropin-releasing hormone (GnRH), a key neuropeptide regulating reproduction in vertebrates has now been characterized in a number of non-vertebrate species. Despite the demonstration of its ancestral origin, the structure and the function of this family of peptides remain poorly known in species as distant as lophotrochozoans. In this study, two GnRH-related peptides (Cg-GnRH-a and CgGnRH-G) were characterized by mass spectrometry from extracts of the visceral ganglia of the Pacific oyster Crassostrea gigas. These peptides showed a high degree of sequence identity with GnRHs of other mollusks and annelids and to a lesser extent with those of vertebrates or with AKH and corazonins of insects. Both the mature peptides and the transcript encoding the precursor protein were exclusively expressed in the visceral ganglia. Significant differences in transcriptional activity of Cg-GnRH encoding gene were recorded in the ganglia along the reproductive cycle and according to trophic conditions with a higher level in fed animals compared to starved animals. This suggests the involvement of Cg-GnRHs as synchronizers of nutritional status with energy requirements during reproduction in oyster. Evidence for a role of Cg-GnRHs as neuroregulators and as neuroendocrine factors in bivalve is discussed. (C) 2012 Elsevier Inc. All rights reserved. |
format |
Article in Journal/Newspaper |
author |
Bigot, Laetitia Zatylny-gaudin, Celine Rodet, Franck Bernay, Benoit Boudry, Pierre Favrel, Pascal |
author_facet |
Bigot, Laetitia Zatylny-gaudin, Celine Rodet, Franck Bernay, Benoit Boudry, Pierre Favrel, Pascal |
author_sort |
Bigot, Laetitia |
title |
Characterization of GnRH-related peptides from the Pacific oyster Crassostrea gigas |
title_short |
Characterization of GnRH-related peptides from the Pacific oyster Crassostrea gigas |
title_full |
Characterization of GnRH-related peptides from the Pacific oyster Crassostrea gigas |
title_fullStr |
Characterization of GnRH-related peptides from the Pacific oyster Crassostrea gigas |
title_full_unstemmed |
Characterization of GnRH-related peptides from the Pacific oyster Crassostrea gigas |
title_sort |
characterization of gnrh-related peptides from the pacific oyster crassostrea gigas |
publisher |
Elsevier Science Inc |
publishDate |
2012 |
url |
https://archimer.ifremer.fr/doc/00079/19003/17477.pdf https://doi.org/10.1016/j.peptides.2012.01.017 https://archimer.ifremer.fr/doc/00079/19003/ |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
Peptides (0196-9781) (Elsevier Science Inc), 2012-04 , Vol. 34 , N. 2 , P. 303-310 |
op_relation |
https://archimer.ifremer.fr/doc/00079/19003/17477.pdf doi:10.1016/j.peptides.2012.01.017 https://archimer.ifremer.fr/doc/00079/19003/ |
op_rights |
2012 Elsevier Inc. All rights reserved. info:eu-repo/semantics/openAccess restricted use |
op_doi |
https://doi.org/10.1016/j.peptides.2012.01.017 |
container_title |
Peptides |
container_volume |
34 |
container_issue |
2 |
container_start_page |
303 |
op_container_end_page |
310 |
_version_ |
1766393737285468160 |