Investigation of marine bivalve morphology by in vivo MR imaging: first anatomical results of a promising technique
Classically, investigation of the internal morphology and composition of molluscs and especially bivalves relies on destructive method (biometry, biochemistry and histology). These techniques have given essential information, but in contrast are time consuming and lead to the irreversible loss of th...
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ftarchimer:oai:archimer.ifremer.fr:1819 2023-05-15T15:59:06+02:00 Investigation of marine bivalve morphology by in vivo MR imaging: first anatomical results of a promising technique Pouvreau, Stephane Rambeau, Marc Cochard, Jean-claude Robert, Rene 2006-09 application/pdf https://archimer.ifremer.fr/doc/2006/publication-1819.pdf https://doi.org/10.1016/j.aquaculture.2006.05.018 https://archimer.ifremer.fr/doc/00000/1819/ eng eng Elsevier https://archimer.ifremer.fr/doc/2006/publication-1819.pdf doi:10.1016/j.aquaculture.2006.05.018 https://archimer.ifremer.fr/doc/00000/1819/ 2006 Elsevier info:eu-repo/semantics/openAccess restricted use Aquaculture (0044-8486) (Elsevier), 2006-09 , Vol. 259 , N. 1-4 , P. 415-423 In vivo physiology Aquaculture Anatomy MRI Pacific oyster text Publication info:eu-repo/semantics/article 2006 ftarchimer https://doi.org/10.1016/j.aquaculture.2006.05.018 2021-09-23T20:14:13Z Classically, investigation of the internal morphology and composition of molluscs and especially bivalves relies on destructive method (biometry, biochemistry and histology). These techniques have given essential information, but in contrast are time consuming and lead to the irreversible loss of the animal while they don't allow integration of the various levels of molecular-to-organism functioning. The aim of this study is to analyze for the first time the potential of NMR (nuclear magnetic resonance) imaging (MRI) to depict, with sufficient resolution and satisfactory contrast, the anatomy of a bivalve model, the Pacific oyster, Crassostrea gigas, without opening it. MRI experiments were carried out at 19 °C in several non-anaesthetized adult Pacific oysters, analyzed individually in a standard General Electric Signa 1.5T (whole body) instrument with actively shielded gradient coils (23 mT/m). To enhance signal detection, the oyster was centered in the middle of a 12-cm diameter Helmholtz-like radio-frequency coil (medical wrist coil). After several trials, the best MRI acquisition sequence retained was a T1-weighted procedure (spoiled gradient echo sequence) through two orthogonal directions (transversal and sagittal sections). According to direction, MR parameters were as follows: TR = 200¿400 ms, TE = 4¿5 ms, FOV = 120 × 90 mm, matrix = 512 × 256 units, 6 signal averages per echo, spatial resolution = 230 μm, total scan time = 3¿6 min. The MR images obtained have satisfactory contrast-to-noise levels, and depict with a sufficient resolution all the main organs in the soft tissues of the oyster. Comparison with histology-based anatomical information shows that the MR images faithfully represent some detailed anatomical structures of Pacific oysters. Potential applications in shellfish aquaculture are reported, and perspectives are given which constitute starting point from furthers studies Article in Journal/Newspaper Crassostrea gigas Pacific oyster Archimer (Archive Institutionnelle de l'Ifremer - Institut français de recherche pour l'exploitation de la mer) Pacific Aquaculture 259 1-4 415 423 |
institution |
Open Polar |
collection |
Archimer (Archive Institutionnelle de l'Ifremer - Institut français de recherche pour l'exploitation de la mer) |
op_collection_id |
ftarchimer |
language |
English |
topic |
In vivo physiology Aquaculture Anatomy MRI Pacific oyster |
spellingShingle |
In vivo physiology Aquaculture Anatomy MRI Pacific oyster Pouvreau, Stephane Rambeau, Marc Cochard, Jean-claude Robert, Rene Investigation of marine bivalve morphology by in vivo MR imaging: first anatomical results of a promising technique |
topic_facet |
In vivo physiology Aquaculture Anatomy MRI Pacific oyster |
description |
Classically, investigation of the internal morphology and composition of molluscs and especially bivalves relies on destructive method (biometry, biochemistry and histology). These techniques have given essential information, but in contrast are time consuming and lead to the irreversible loss of the animal while they don't allow integration of the various levels of molecular-to-organism functioning. The aim of this study is to analyze for the first time the potential of NMR (nuclear magnetic resonance) imaging (MRI) to depict, with sufficient resolution and satisfactory contrast, the anatomy of a bivalve model, the Pacific oyster, Crassostrea gigas, without opening it. MRI experiments were carried out at 19 °C in several non-anaesthetized adult Pacific oysters, analyzed individually in a standard General Electric Signa 1.5T (whole body) instrument with actively shielded gradient coils (23 mT/m). To enhance signal detection, the oyster was centered in the middle of a 12-cm diameter Helmholtz-like radio-frequency coil (medical wrist coil). After several trials, the best MRI acquisition sequence retained was a T1-weighted procedure (spoiled gradient echo sequence) through two orthogonal directions (transversal and sagittal sections). According to direction, MR parameters were as follows: TR = 200¿400 ms, TE = 4¿5 ms, FOV = 120 × 90 mm, matrix = 512 × 256 units, 6 signal averages per echo, spatial resolution = 230 μm, total scan time = 3¿6 min. The MR images obtained have satisfactory contrast-to-noise levels, and depict with a sufficient resolution all the main organs in the soft tissues of the oyster. Comparison with histology-based anatomical information shows that the MR images faithfully represent some detailed anatomical structures of Pacific oysters. Potential applications in shellfish aquaculture are reported, and perspectives are given which constitute starting point from furthers studies |
format |
Article in Journal/Newspaper |
author |
Pouvreau, Stephane Rambeau, Marc Cochard, Jean-claude Robert, Rene |
author_facet |
Pouvreau, Stephane Rambeau, Marc Cochard, Jean-claude Robert, Rene |
author_sort |
Pouvreau, Stephane |
title |
Investigation of marine bivalve morphology by in vivo MR imaging: first anatomical results of a promising technique |
title_short |
Investigation of marine bivalve morphology by in vivo MR imaging: first anatomical results of a promising technique |
title_full |
Investigation of marine bivalve morphology by in vivo MR imaging: first anatomical results of a promising technique |
title_fullStr |
Investigation of marine bivalve morphology by in vivo MR imaging: first anatomical results of a promising technique |
title_full_unstemmed |
Investigation of marine bivalve morphology by in vivo MR imaging: first anatomical results of a promising technique |
title_sort |
investigation of marine bivalve morphology by in vivo mr imaging: first anatomical results of a promising technique |
publisher |
Elsevier |
publishDate |
2006 |
url |
https://archimer.ifremer.fr/doc/2006/publication-1819.pdf https://doi.org/10.1016/j.aquaculture.2006.05.018 https://archimer.ifremer.fr/doc/00000/1819/ |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
Aquaculture (0044-8486) (Elsevier), 2006-09 , Vol. 259 , N. 1-4 , P. 415-423 |
op_relation |
https://archimer.ifremer.fr/doc/2006/publication-1819.pdf doi:10.1016/j.aquaculture.2006.05.018 https://archimer.ifremer.fr/doc/00000/1819/ |
op_rights |
2006 Elsevier info:eu-repo/semantics/openAccess restricted use |
op_doi |
https://doi.org/10.1016/j.aquaculture.2006.05.018 |
container_title |
Aquaculture |
container_volume |
259 |
container_issue |
1-4 |
container_start_page |
415 |
op_container_end_page |
423 |
_version_ |
1766394882310537216 |