A combined microsatellite multiplexing and boiling DNA extraction method for high-throughput parentage analyses in the Pacific oyster (Crassostrea gigas)
The analysis of parentage using microsatellite markers is of increasing importance, notably in aquaculture genetic research where communal rearing of mixed families can be used to reduce unwanted environmental variance. We present here an optimization of parental genotype assessment for larvae or ad...
Published in: | Aquaculture Research |
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Format: | Article in Journal/Newspaper |
Language: | English |
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Blackwell science
2005
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Online Access: | https://archimer.ifremer.fr/doc/2005/publication-1814.pdf https://doi.org/10.1111/j.1365-2109.2004.01208.x https://archimer.ifremer.fr/doc/00000/1814/ |
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ftarchimer:oai:archimer.ifremer.fr:1814 2023-05-15T15:57:41+02:00 A combined microsatellite multiplexing and boiling DNA extraction method for high-throughput parentage analyses in the Pacific oyster (Crassostrea gigas) Taris, Nicolas Baron, S Sharbel, Tim Sauvage, Christopher Boudry, Pierre 2005-03 application/pdf https://archimer.ifremer.fr/doc/2005/publication-1814.pdf https://doi.org/10.1111/j.1365-2109.2004.01208.x https://archimer.ifremer.fr/doc/00000/1814/ eng eng Blackwell science https://archimer.ifremer.fr/doc/2005/publication-1814.pdf doi:10.1111/j.1365-2109.2004.01208.x https://archimer.ifremer.fr/doc/00000/1814/ 2005 Blackwell Publishing, Inc. info:eu-repo/semantics/openAccess restricted use Aquaculture Research (1355-557X) (Blackwell science), 2005-03 , Vol. 36 , N. 5 , P. 516-518 Oysters Aquaculture Parental assignment High throughput Larval DNA extraction Multiplex text Publication info:eu-repo/semantics/article 2005 ftarchimer https://doi.org/10.1111/j.1365-2109.2004.01208.x 2021-09-23T20:14:13Z The analysis of parentage using microsatellite markers is of increasing importance, notably in aquaculture genetic research where communal rearing of mixed families can be used to reduce unwanted environmental variance. We present here an optimization of parental genotype assessment for larvae or adults of the Pacific oyster, Crassostrea gigas, using a multiplex system of three microsatellite loci. In conjunction with a simple DNA extraction protocol, this method enables high throughput analyses of parentage in C. gigas. Using this method, we successfully determined the parentage of 93% (1224 out of 1318) of the progeny in a factorial cross between 3 females and 10 males. The inability to genotype the remaining 7% was due to DNA degradation of larvae rather than assignment uncertainty. Article in Journal/Newspaper Crassostrea gigas Pacific oyster Archimer (Archive Institutionnelle de l'Ifremer - Institut français de recherche pour l'exploitation de la mer) Pacific Aquaculture Research 36 5 516 518 |
institution |
Open Polar |
collection |
Archimer (Archive Institutionnelle de l'Ifremer - Institut français de recherche pour l'exploitation de la mer) |
op_collection_id |
ftarchimer |
language |
English |
topic |
Oysters Aquaculture Parental assignment High throughput Larval DNA extraction Multiplex |
spellingShingle |
Oysters Aquaculture Parental assignment High throughput Larval DNA extraction Multiplex Taris, Nicolas Baron, S Sharbel, Tim Sauvage, Christopher Boudry, Pierre A combined microsatellite multiplexing and boiling DNA extraction method for high-throughput parentage analyses in the Pacific oyster (Crassostrea gigas) |
topic_facet |
Oysters Aquaculture Parental assignment High throughput Larval DNA extraction Multiplex |
description |
The analysis of parentage using microsatellite markers is of increasing importance, notably in aquaculture genetic research where communal rearing of mixed families can be used to reduce unwanted environmental variance. We present here an optimization of parental genotype assessment for larvae or adults of the Pacific oyster, Crassostrea gigas, using a multiplex system of three microsatellite loci. In conjunction with a simple DNA extraction protocol, this method enables high throughput analyses of parentage in C. gigas. Using this method, we successfully determined the parentage of 93% (1224 out of 1318) of the progeny in a factorial cross between 3 females and 10 males. The inability to genotype the remaining 7% was due to DNA degradation of larvae rather than assignment uncertainty. |
format |
Article in Journal/Newspaper |
author |
Taris, Nicolas Baron, S Sharbel, Tim Sauvage, Christopher Boudry, Pierre |
author_facet |
Taris, Nicolas Baron, S Sharbel, Tim Sauvage, Christopher Boudry, Pierre |
author_sort |
Taris, Nicolas |
title |
A combined microsatellite multiplexing and boiling DNA extraction method for high-throughput parentage analyses in the Pacific oyster (Crassostrea gigas) |
title_short |
A combined microsatellite multiplexing and boiling DNA extraction method for high-throughput parentage analyses in the Pacific oyster (Crassostrea gigas) |
title_full |
A combined microsatellite multiplexing and boiling DNA extraction method for high-throughput parentage analyses in the Pacific oyster (Crassostrea gigas) |
title_fullStr |
A combined microsatellite multiplexing and boiling DNA extraction method for high-throughput parentage analyses in the Pacific oyster (Crassostrea gigas) |
title_full_unstemmed |
A combined microsatellite multiplexing and boiling DNA extraction method for high-throughput parentage analyses in the Pacific oyster (Crassostrea gigas) |
title_sort |
combined microsatellite multiplexing and boiling dna extraction method for high-throughput parentage analyses in the pacific oyster (crassostrea gigas) |
publisher |
Blackwell science |
publishDate |
2005 |
url |
https://archimer.ifremer.fr/doc/2005/publication-1814.pdf https://doi.org/10.1111/j.1365-2109.2004.01208.x https://archimer.ifremer.fr/doc/00000/1814/ |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
Aquaculture Research (1355-557X) (Blackwell science), 2005-03 , Vol. 36 , N. 5 , P. 516-518 |
op_relation |
https://archimer.ifremer.fr/doc/2005/publication-1814.pdf doi:10.1111/j.1365-2109.2004.01208.x https://archimer.ifremer.fr/doc/00000/1814/ |
op_rights |
2005 Blackwell Publishing, Inc. info:eu-repo/semantics/openAccess restricted use |
op_doi |
https://doi.org/10.1111/j.1365-2109.2004.01208.x |
container_title |
Aquaculture Research |
container_volume |
36 |
container_issue |
5 |
container_start_page |
516 |
op_container_end_page |
518 |
_version_ |
1766393369925255168 |